Structure of PDB 1z2p Chain X

Receptor sequence

>1z2pX (length=311) Species: 294381 (Entamoeba histolytica HM-1:IMSS)

QTVSLFIWLPESKQKTLFISTKNHTQFELNNIIFDVTLSTELPDKEPNAI
ITKRTHPVGKMADEMRKYEKDHPKVLFLESSAIHDMMSSREEINALLIKN
NIPIPNSFSVKSKEEVIQLLQSKQLILPFIVKPENAQGTFNAHQMKIVLE
QEGIDDIHFPCLCQHYINHNNKIVKVFCIGNTLKWQTRTSLPNVHRCGIK
SVDFNNQHLEDILSWPEGVIDKQDIIENSANRFGSKILEDPILLNLTSEA
EMRDLAYKVRCALGVQLCGIDFIKENEQGNPLVVDVNVFPSYGGKVDFDW
FVEKVALCYTE

3D structure

• PDB: 1z2p Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase.
• Chain: X
• Resolution: 1.22 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.134: inositol-tetrakisphosphate 1-kinase.
  2.7.1.159: inositol-1,3,4-trisphosphate 5/6-kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	X	D275 D289	D271 D285
BS02	MG	X	D289 N291	D285 N287
BS03	ACP	X	R94 K136 H147 M149 Q168 Y170 I171 H173 S194 L195 N210 D275 V288 D289 N291	R90 K132 H143 M145 Q164 Y166 I167 H169 S190 L191 N206 D271 V284 D285 N287
BS04	I3S	X	K17 K57 Q141 G142 H147 K179 N291 S295	K13 K53 Q137 G138 H143 K175 N287 S291

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding
• GO:0047325 inositol-3,4,5,6-tetrakisphosphate 1-kinase activity
• GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
• GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity

Biological Process

• GO:0016310 phosphorylation
• GO:0032957 inositol trisphosphate metabolic process

External links

• PDB: RCSB:1z2p, PDBe:1z2p, PDBj:1z2p
• PDBsum: 1z2p
• PubMed: 15837423
• UniProt: Q9XYQ1|ITPK1_ENTH1 Inositol-tetrakisphosphate 1-kinase (Gene Name=ITPK1)