●I-TASSER ●I-TASSER-MTD ●C-I-TASSER ●CR-I-TASSER ●QUARK ●C-QUARK ●LOMETS ●MUSTER ●CEthreader ●SEGMER ●DeepFold ●DeepFoldRNA ●FoldDesign ●COFACTOR ●COACH ●MetaGO ●TripletGO ●IonCom ●FG-MD ●ModRefiner ●REMO ●DEMO ●DEMO-EM ●SPRING ●COTH ●Threpp ●PEPPI ●BSpred ●ANGLOR ●EDock ●BSP-SLIM ●SAXSTER ●FUpred ●ThreaDom ●ThreaDomEx ●EvoDesign ●BindProf ●BindProfX ●SSIPe ●GPCR-I-TASSER ●MAGELLAN ●ResQ ●STRUM ●DAMpred

●TM-score ●TM-align ●US-align ●MM-align ●RNA-align ●NW-align ●LS-align ●EDTSurf ●MVP ●MVP-Fit ●SPICKER ●HAAD ●PSSpred ●3DRobot ●MR-REX ●I-TASSER-MR ●SVMSEQ ●NeBcon ●ResPRE ●TripletRes ●DeepPotential ●WDL-RF ●ATPbind ●DockRMSD ●DeepMSA ●FASPR ●EM-Refiner ●GPU-I-TASSER

●BioLiP ●E. coli ●GLASS ●GPCR-HGmod ●GPCR-RD ●GPCR-EXP ●Tara-3D ●TM-fold ●DECOYS ●POTENTIAL ●RW/RWplus ●EvoEF ●HPSF ●THE-DB ●ADDRESS ●Alpaca-Antibody ●CASP7 ●CASP8 ●CASP9 ●CASP10 ●CASP11 ●CASP12 ●CASP13 ●CASP14

SPRING is a template-base algorithm for protein-protein complex structure prediction. The pipeline first threads one chain of the protein complex through the PDB library with the binding parters retrieved from the original oligomer entries. The complex models for the query are then deduced from the template binding partner associations through a pre-calculated look-up table. The model of the best orientation is finally selected by the SPRING-score that is a combination of threading Z-score, interface contacts, and TM-align match between monomer-to-dimer templates.

The output of the SPRING server include (An example of the SPRING output):

• Top ten complex template alignments.
• SPRING recombination models of tertiary template with quaternary framework.

SPRING On-Line Server

Cut and paste your first sequence in FASTA format here:


Or upload the sequence from your local computer:

Cut and paste your second sequence in FASTA format here:


Or upload the sequence from your local computer:

Email: (mandatory, where results will be sent to)

ID: (optional, your given name of the protein)

SPRING Download

• Click here to download the standalone package of SPRING progams and the template libarary.
• Click ecoli_spring.zip to download structural models of all protein-protein interactions predicted by spring in E. coli genome.

Reference:

Aysam Guerler, Brandon Govindarajoo, Yang Zhang. Mapping Monomeric Threading to Protein-Protein Structure Prediction. Journal of Chemical Information and Modeling, 53, 717-725 (2013) (Download PDF)