Structure of PDB 5akm Chain F

Receptor sequence

>5akmF (length=174) [Search protein sequence]

NVSGISAYLLGLIISDGGLYKLKYKGNRSEYRVVITQKSENLIKQHIAPL
MQFLIDELNVKSKIQIVKGDTRYELRVSSKKLYYYFANMLERIRLFNMRE
QIAFIKGLYVAEGDKTLKRLRIWNKNKALLEIVSRWLNNLGVRNTIHLDD
HRHGVYVLNISLRDRIKFVHTILS

3D structure

PDB

5akm Engineering a Nickase on the Homing Endonuclease I-Dmoi Scaffold.

Chain

Resolution

2.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	D21 T41 Q42 K43 T76 R77 R124 T150 H152 D154 H156 R157 N164 S166 L167	D16 T36 Q37 K38 T71 R72 R119 T145 H147 D149 H151 R152 N159 S161 L162
BS02	dna	F	S20 G22 Y25 Y29 K30 R33 R77	S15 G17 Y20 Y24 K25 R28 R72
BS03	dna	F	N32 R33 S34 E35 Y36 R37 S67 K68 Q70 R81 S83 K85 E117 W128 N129 K130 H158 V160	N27 R28 S29 E30 Y31 R32 S62 K63 Q65 R76 S78 K80 E112 W123 N124 K125 H153 V155
BS04	dna	F	A116 D119 K120 R126 W128	A111 D114 K115 R121 W123
BS05	dna	F	K86 Y89 N93 R97	K81 Y84 N88 R92
BS06	MG	F	D21 A116	D16 A111
BS07	MG	F	S20 E117	S15 E112

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

	Biological Process
GO:0006314	intron homing
GO:0016539	intein-mediated protein splicing

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Molecular Function

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Biological Process

External links

PDB	RCSB:5akm, PDBe:5akm, PDBj:5akm
PDBsum	5akm
PubMed	26045557
UniProt	P21505\|DMO1_DESMO Homing endonuclease I-DmoI

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