Structure of PDB 4zcf Chain C

Receptor sequence

>4zcfC (length=627) Species: 562 (Escherichia coli)

TLEKNLPHQKAGVDAVMNVFVSATPHLTDNVAVRLLANPELKLSEQQYYN
NIKNVQAFNGIAHSKDNHNAKSNIIDVSMETGTGKTYTYIKTIFDLNKSF
GINKFIIIVPTLSIKAGTVNFLKSDALKEHFRDDYKRELRTYVVESQKMP
QAIHDFVEASNFKKYIHVLVINSGMINSKSLTDTYDTGLLDNQFNTPVDA
LRAVKPFIIIDEPHRFPTGKKTWENIEKFNAQYIIRYGATFSEGYKNLVY
RLTAVDAFNDDLVKGIDAYIEDNANLKFVKDGKEATFFKLAKSLSKTHSA
IHDLTLDALNTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKL
EKELLTQPRIKPLTLFFIDDLKTKFEEYVLAEANELLYKNYLEKTVTNIS
SVHGGYIEQEINEILHDKELLLSLDNPRRFIFSKWTLREGWDNPNVFQIC
KLRSSKLQEVGRGLRLPVNEYMCRVKNFTLKYYVDFTEKDFVDSLVKEVN
ESSPSKFTQELKEQIDNFKDSDAYSRLKSELKELWDLINQKAVIEYKINS
ESEFLSIFKSFMLEETERSYREFLDNLSQTIFVKHGTLHKVFCDIKDTIL
NIQTIRKIKSGFSKYLLNNSFSLGYNL

3D structure

• PDB: 4zcf Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I.
• Chain: C
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.5: type III site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	C	N192 R230 K235 K236 T237	N177 R215 K220 K221 T222
BS02	dna	C	T116 L117 S151 N187 G189 M190 S193 K194 G352 I353 E354	T111 L112 S146 N172 G174 M175 S178 K179 G317 I318 E319
BS03	AMP	C	T91 I119 G122 F126 D226 R537	T86 I114 G117 F121 D211 R465

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004386 helicase activity
• GO:0004519 endonuclease activity
• GO:0005524 ATP binding
• GO:0015668 type III site-specific deoxyribonuclease activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0009307 DNA restriction-modification system

External links

• PDB: RCSB:4zcf, PDBe:4zcf, PDBj:4zcf
• PDBsum: 4zcf
• PubMed: 26067164
• UniProt: Q5ZND2|T3RE_ECOLX Type III restriction-modification enzyme EcoP15I Res subunit (Gene Name=res)