BioLiP

Structure of PDB 2nr0 Chain C

Receptor sequence

>2nr0C (length=264) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

PPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPITVFCA
GRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKTVPDD
FHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLL
GENDFTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRN
IVGSLMEVGAHNQPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRY
DLPKPPMGPLFLAD

3D structure

PDB

2nr0 How U38, 39, and 40 of Many tRNAs Become the Targets for Pseudouridylation by TruA.

Chain

Resolution

3.9 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.4.99.12: tRNA pseudouridine(38-40) synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	C	Y24 R28 Q29 N30 R58 D60 A61 R110 R126 Y140 V167 S171 R172 T173 H202 A238 T239	Y18 R22 Q23 N24 R52 D54 A55 R104 R120 Y134 V161 S165 R166 T167 H196 A232 T233	PDBbind-CN: Kd=1.05uM
BS02	rna	C	V46 N48 R78 A82 L85 A89 N90	V40 N42 R72 A76 L79 A83 N84	PDBbind-CN: Kd=1.05uM

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity
GO:0016853	isomerase activity
GO:0042803	protein homodimerization activity
GO:0106029	tRNA pseudouridine synthase activity
GO:0140098	catalytic activity, acting on RNA
GO:0160147	tRNA pseudouridine(38-40) synthase activity

	Biological Process
GO:0001522	pseudouridine synthesis
GO:0006396	RNA processing
GO:0008033	tRNA processing
GO:0009451	RNA modification
GO:0031119	tRNA pseudouridine synthesis

View graph for
Molecular Function

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Biological Process

PDB	RCSB:2nr0, PDBe:2nr0, PDBj:2nr0
PDBsum	2nr0
PubMed	17466622
UniProt	P07649\|TRUA_ECOLI tRNA pseudouridine synthase A (Gene Name=truA)