BioLiP

Structure of PDB 3efs Chain B

Receptor sequence

>3efsB (length=232) Species: 63363 (Aquifex aeolicus) [Search protein sequence]

FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGRLGRKWLSQEGG
LYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQE
KKVSGVLRELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRK
EVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITG
KLVGLSEKGGALILTEEGIKEILSGEFSLRRS

3D structure

PDB

3efs Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.

Chain

Resolution

2.3 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R40 K103 R231
Catalytic site (residue number reindexed from 1)	R39 K102 R230
Enzyme Commision number	6.3.4.15: biotin--[biotin carboxyl-carrier protein] ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	B	K192 S233	K191 S232	MOAD: Kd=7.2uM
BS02	ATP	B	R40 R43 W45 L46 K103 N123 S229	R39 R42 W44 L45 K102 N122 S228	MOAD: Kd=7.2uM
BS03	BTN	B	S13 T14 Q15 Q34 G37 R38 R40 W45 Y53 F54 G106 G119 I120 G121	S12 T13 Q14 Q33 G36 R37 R39 W44 Y52 F53 G105 G118 I119 G120	MOAD: Kd=3.5uM

Gene Ontology

	Molecular Function
GO:0004077	biotin--[biotin carboxyl-carrier protein] ligase activity
GO:0005524	ATP binding
GO:0016874	ligase activity

	Biological Process
GO:0036211	protein modification process

	Cellular Component
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

PDB	RCSB:3efs, PDBe:3efs, PDBj:3efs
PDBsum	3efs
PubMed	19385043
UniProt	O66837