Structure of PDB 3efs Chain B

Receptor sequence
>3efsB (length=232) Species: 63363 (Aquifex aeolicus) [Search protein sequence]
FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGRLGRKWLSQEGG
LYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQE
KKVSGVLRELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRK
EVLLKVLKRISENLKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITG
KLVGLSEKGGALILTEEGIKEILSGEFSLRRS
3D structure
PDB3efs Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.
ChainB
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R40 K103 R231
Catalytic site (residue number reindexed from 1) R39 K102 R230
Enzyme Commision number 6.3.4.15: biotin--[biotin carboxyl-carrier protein] ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP B K192 S233 K191 S232 MOAD: Kd=7.2uM
BS02 ATP B R40 R43 W45 L46 K103 N123 S229 R39 R42 W44 L45 K102 N122 S228 MOAD: Kd=7.2uM
BS03 BTN B S13 T14 Q15 Q34 G37 R38 R40 W45 Y53 F54 G106 G119 I120 G121 S12 T13 Q14 Q33 G36 R37 R39 W44 Y52 F53 G105 G118 I119 G120 MOAD: Kd=3.5uM
Gene Ontology
Molecular Function
GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
Biological Process
GO:0036211 protein modification process
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:3efs, PDBe:3efs, PDBj:3efs
PDBsum3efs
PubMed19385043
UniProtO66837

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