Structure of PDB 8wa7 Chain A

Receptor sequence

>8wa7A (length=663) Species: 562 (Escherichia coli)

MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQ
NPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGH
PEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTY
AFMGDGCMMEGISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDD
TAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSLLMCKTIIGFG
SPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAG
QAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMPSDFDAKAKEFIAKLQA
NPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESA
VAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQP
ELIFIATGSEVELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESV
LPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAPAELLFEEFGF
TVDNVVAKAKELL

3D structure

• PDB: 8wa7 Multifaceted Role of the Substrate Phosphate Group in Transketolase Catalysis
• Chain: A
• Resolution: 1.63 Å

Enzymatic activity

• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	THD	A	H66 H100 L116 D155 G156 N185 I187 I189 H261	H66 H100 L116 D155 G156 N185 I187 I189 H261
BS02	MG	A	D155 N185 I187	D155 N185 I187
BS03	THD	A	D381 E411 F437 Y440 H473	D381 E411 F437 Y440 H473

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0016740 transferase activity
• GO:0030145 manganese ion binding
• GO:0030976 thiamine pyrophosphate binding
• GO:0042803 protein homodimerization activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt
• GO:0009052 pentose-phosphate shunt, non-oxidative branch

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8wa7, PDBe:8wa7, PDBj:8wa7
• PDBsum: 8wa7
• UniProt: P27302|TKT1_ECOLI Transketolase 1 (Gene Name=tktA)