Structure of PDB 2ge5 Chain A

Receptor sequence
>2ge5A (length=219) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYE
3D structure
PDB2ge5 Positively Charged C-Terminal Subdomains of EcoRV Endonuclease: Contributions to DNA Binding, Bending, and Cleavage.
ChainA
Resolution2.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T37 N70 D90 K92 T93 T94 Y95 G109 T111 S112 K119 G182 S183 T186 T36 N69 D89 K91 T92 T93 Y94 G108 T110 S111 K118 G181 S182 T185 PDBbind-CN: Kd=381nM
BS02 dna A Q69 N70 L180 S183 G184 N185 Q68 N69 L179 S182 G183 N184 PDBbind-CN: Kd=381nM
BS03 CA A D74 D90 D73 D89
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity

View graph for
Molecular Function
External links
PDB RCSB:2ge5, PDBe:2ge5, PDBj:2ge5
PDBsum2ge5
PubMed16981705
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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