Structure of PDB 2ge5 Chain A

Receptor sequence

>2ge5A (length=219) Species: 562 (Escherichia coli) [Search protein sequence]

SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYE

3D structure

PDB

2ge5 Positively Charged C-Terminal Subdomains of EcoRV Endonuclease: Contributions to DNA Binding, Bending, and Cleavage.

Chain

Resolution

2.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.21.4: type II site-specific deoxyribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T37 N70 D90 K92 T93 T94 Y95 G109 T111 S112 K119 G182 S183 T186	T36 N69 D89 K91 T92 T93 Y94 G108 T110 S111 K118 G181 S182 T185	PDBbind-CN: Kd=381nM
BS02	dna	A	Q69 N70 L180 S183 G184 N185	Q68 N69 L179 S182 G183 N184	PDBbind-CN: Kd=381nM
BS03	CA	A	D74 D90	D73 D89

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0004519	endonuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:2ge5, PDBe:2ge5, PDBj:2ge5
PDBsum	2ge5
PubMed	16981705
UniProt	P04390\|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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