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Structure of PDB 1e22 Chain A

Receptor sequence
>1e22A (length=485) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
AIDFNDELRNRREKLAALRQQGVAFPNDFRRDHTSDQLHEEFDAKDNQEL
ESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDSLPEGVYND
QFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDQEVRYR
QRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGIS
VRHNPEFTMMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVF
DFGKPFEKLTMREAIKKYRPETDMADLDNFDAAKALAESIGITVEKSWGL
GRIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFI
GGREIGNGFSELNDAEDQAERFQEQVNAKAAGDDEAMFYDEDYVTALEYG
LPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRP
3D structure
PDB1e22 Active Site of Lysyl-tRNA Synthetase: Structural Studies of the Adenylation Reaction
ChainA
Resolution2.43 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R262 E264 R269 H270 E421 N424 R480
Catalytic site (residue number reindexed from 1) R245 E247 R252 H253 E404 N407 R463
Enzyme Commision number 6.1.1.6: lysine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 LYS A G216 E240 M276 E278 Y280 N424 F426 E428 G473 G475 G199 E223 M259 E261 Y263 N407 F409 E411 G456 G458
BS02 ACP A R262 R269 H270 N271 F274 E421 I422 G477 R480 R245 R252 H253 N254 F257 E404 I405 G460 R463
BS03 MG A E414 E421 E397 E404
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004824 lysine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006430 lysyl-tRNA aminoacylation
GO:0034605 cellular response to heat
GO:0036260 RNA capping
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane

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Cellular Component
External links
PDB RCSB:1e22, PDBe:1e22, PDBj:1e22
PDBsum1e22
PubMed10913247
UniProtP0A8N5|SYK2_ECOLI Lysine--tRNA ligase, heat inducible (Gene Name=lysU)

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