Structure of PDB 1af2 Chain A

Receptor sequence
>1af2A (length=294) Species: 562 (Escherichia coli) [Search protein sequence]
MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSATGLDED
ALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQT
VHAEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLP
GREAHALRDYLPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAAN
RSHMPYSKSPSGVALECKDGRIFSGSYAENAAFNPTLPPLQGALILLNLK
GYDYPDIQRAVLAEKADAPLIQWDATSATLKALGCHSIDRVLLA
3D structure
PDB1af2 The structure of the cytidine deaminase-product complex provides evidence for efficient proton transfer and ground-state destabilization.
ChainA
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H102 E104 C129 C132
Catalytic site (residue number reindexed from 1) H102 E104 C129 C132
Enzyme Commision number 3.5.4.5: cytidine deaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A H102 C129 C132 H102 C129 C132
BS02 U A N89 E91 H102 E104 P128 C129 N89 E91 H102 E104 P128 C129 PDBbind-CN: -logKd/Ki=3.10,Ki=0.8mM
Gene Ontology
Molecular Function
GO:0001884 pyrimidine nucleoside binding
GO:0003824 catalytic activity
GO:0004126 cytidine deaminase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006217 deoxycytidine catabolic process
GO:0009972 cytidine deamination
GO:0015949 nucleobase-containing small molecule interconversion
GO:0055086 nucleobase-containing small molecule metabolic process
GO:0072527 pyrimidine-containing compound metabolic process
GO:1901135 carbohydrate derivative metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:1af2, PDBe:1af2, PDBj:1af2
PDBsum1af2
PubMed9125497
UniProtP0ABF6|CDD_ECOLI Cytidine deaminase (Gene Name=cdd)

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