Structure of PDB 3jce Chain 5

Receptor sequence

>3jce5 (length=234) Species: 83333 (Escherichia coli K-12)

MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGI
DARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDL
ADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVA
EAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAK
PTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN

3D structure

• PDB: 3jce EF4 disengages the peptidyl-tRNA CCA end and facilitates back-translocation on the 70S ribosome
• Chain: 5
• Resolution: 3.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	5	M1 K3 L4 T5 K6 R7 M8 I11 K14 T35 K37 F38 A45 R71 I104 K105 K106 G107 E108 M109 N110 F111 D112 L127 Q129 V130 P133 R134 G135 L136 N139 P140 D166 K167 N168 I170 H172 T174 S213 S215 T216 T217 M218 G221	M1 K3 L4 T5 K6 R7 M8 I11 K14 T35 K37 F38 A45 R71 I104 K105 K106 G107 E108 M109 N110 F111 D112 L127 Q129 V130 P133 R134 G135 L136 N139 P140 D166 K167 N168 I170 H172 T174 S213 S215 T216 T217 M218 G221
BS02	rna	5	R53 K54 Q129 K141	R53 K54 Q129 K141

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0045947 negative regulation of translational initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3jce, PDBe:3jce, PDBj:3jce
• PDBsum: 3jce
• PubMed: 26809121
• UniProt: P0A7L0|RL1_ECOLI Large ribosomal subunit protein uL1 (Gene Name=rplA)