Structure of PDB 8wi8 Chain X Binding Site BS01

Receptor Information
>8wi8 Chain X (length=93) Species: 246196 (Mycolicibacterium smegmatis MC2 155) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MKVHKGDTVLVISGKDKGAKGKVLVAYPDRNKVLVEGVNRIKKHTGIVTQ
EAPIHVSNVMVVDSDGKPTRVGYRIDDETGKKVRIAKTNGKDI
Ligand information
>8wi8 Chain A (length=3025) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaacacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaugcaugggaccuucguugguaguagucaagcgaugggg
ugacgcaggaagguagccguaccggucagugguaauaccgggguaagccu
guagggagucagauagguaaauccgucuggcauauauccugagaggugau
gcauagccgagugaggcgaauucggugauccuaugcugccgagaaaagcc
ucuagcgaggacauacacggcccguaccccaaaccaacacagguggucag
guagagaauacuaaggcguacgagugaacuaugguuaaggaacucggcaa
aaugcccccguaacuucgggagaagggggacccacauggcguguaagccu
uuacggcccaagcgugaguggguggcacaaaccagugagaagcgacuguu
uacuaaaaacacagguccgugcgaagucgcaagacgauguauacggacug
acgccugcccggugcuggaagguuaagaggacccguuaacucccuuuggg
ggugaagcggagaauuuaagccccaguaaacggcggugguaacuauaacc
auccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaau
ggcguaacgacuucucaacugucucaaccauagacucggcgaaauugcac
uacgaguaaagaugcucguuacgcgcggcaggacgaaaagaccccgggac
cuucacuacaacuugguauuggugcucgauacgguuuguguaggauaggu
gggagacugugaagcucacacgccaguguggguggagucguuguugaaau
accacucugaucguauugggccucuaaccucggaccguauauccgguuca
gggacagugccugguggguaguuuaacuggggcgguugccuccuaaaaug
uaacggaggcgcccaaagguucccucaaccuggacggcaaucagguguug
aguguaagugcacaagggagcuugacugcgagacggacaugucgagcagg
gacgaaagucgggacuagugauccggcaccucugaguggaaggggugucg
cucaacggauaaaagguaccccggggauaacaggcugaucuuccccaaga
guccauaucgacgggaugguuuggcaccucgaugucggcucgucgcaucc
uggggcuggagcaggucccaaggguugggcuguucgcccauuaaagcggc
acgcgagcuggguuuagaacgucgugagacaguucggucucuauccgccg
cgcgcgucagaagcuugaggaaaccugucccuaguacgagaggaccggga
cggacgaaccucugguauaccaguugucccaccaggggcacggcuggaua
gccacguucggacaggauaaccgcugaaagcaucuaagcgggaaaccucu
uccaagaccaggcuucucacccucuaggagggauaaggccccccgcagac
cacgggauugauagaccagaccuggaagccuaguaauaggugcagggaac
uggcacuaaccggccgaaaacuuac
<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........(((((....
.>>>>>>>>>>......<<<<..<.....<<<<<<<))))).>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....>.
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<.<<......<<<<
....>>>>.......>>.>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<......>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
.....<<<<......>>>>.....>>>>>>>>....>>>>.<<...<<<<
<...<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<
...<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...
>>...<<<......>>>.....>>>>...>>>...>>>>........>>>
>>..>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<
<<<.....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.
....<<<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<.
....>>>....>>>>..>>>>>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.
<<<<<.<<<..<<<<<<<..<<<..<<<....<<<<<.<<<<<<...>>>
>>>..>>>>>....>>>..>>>>>>>>..>>>>><<<<<<.......>>>
>>>........<...........<<<<<<.....>>>>>>.....>....
>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((.
.<<<<<......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<
<<..<<<<<.<<<.<<<<<.<<.<<<<<<<<<<<<..............<
<<..<<<......<<<<<<<<....>>>>>>>>...>>>...........
.>>>.....>>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>
>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.
<<<....>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>
.............>>>>..>>>>>>.......<<<<...<.<<<<...<<
.......>>...>>>>.>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<<...............>>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...
>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>
>>>.......>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.....
..>>>>.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>
>>>..>>>...>>>>....>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8wi8 Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
Resolution2.7 Å
Binding residue
(original residue number in PDB)		K2 H4 G14 K15 P28 I41 K42 K43 H44 T45 H67 S69 N70 M72 P80 R82 Y85 K93 K94 K99
Binding residue
(residue number reindexed from 1)		K2 H4 G14 K15 P28 I41 K42 K43 H44 T45 H55 S57 N58 M60 P68 R70 Y73 K81 K82 K87
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8wi8, PDBe:8wi8, PDBj:8wi8
PDBsum8wi8
PubMed38245551
UniProtA0QSG0|RL24_MYCS2 Large ribosomal subunit protein uL24 (Gene Name=rplX)

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