Structure of PDB 6ha1 Chain N Binding Site BS01

Receptor Information

>6ha1 Chain N (length=119) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

SYRKLGRTSAQRKAMLRDLTTDLIINERIETTETRAKELRSVVEKMITLG
KRGDLHARRQAAAYIRNEVANEENNQDALQKLFSDIATRYEERQGGYTRI
MKLGPRRGDGAPMAIIELV

Ligand information

>6ha1 Chain A (length=2887)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucgaacgagguagaugaagaggucuggaaaggcccgccauagg
agguaacagcccuguagucaaaacuucguucgaguggauccugaguacgg
cggaacacgugaaauuccgucggaauccgggaggaccaucucccaaggcu
aaauacucccuagugaccgauagugaaccaguaccgugagggaaagguga
aaagcaccccggaaggggagugaaagagauccugaaaccgugugccuaca
aguagucagagcccguuaacgggugauggcgugccuuuuguagaaugaac
cggcgaguuacgaucucgugcaagguuaagcagaagaugcggagccgcag
cgaaagcgagucugaauagggcgcaugaguacguggucguagacccgaaa
ccaggugaucuacccauguccagggugaaguucagguaacacugaaugga
ggcccgaacccacgcacguugaaaagugcggggaugagguguggguaggg
gugaaaugccaaucgaaccuggagauagcugguucucuccgaaauagcuu
uagggcuagccucaagguaagagucuuggagguagagcacugauuggacu
aggggccccuaccggguuaccgaauucagucaaacuccgaaugccaauga
cuuauccuugggagucagacugcgagugauaagauccguagucgaaaggg
aaacagcccagaccgccagcuaaggucccaaaguauacguuaaguggaaa
aggauguggaguugcuuagacaaccaggauguuggcuuagaagcagccac
cauuuaaagagugcguaauagcucacuggucgagugacucugcgccgaaa
auguaccggggcuaaacguaucaccgaagcugcggacuguucgaacagug
guaggagagcguucuaagggcugugaagccagaccggaaggacuggugga
gcgcuuagaagugagaaugccgguaugaguagcgaaagaggggugagaau
ccccuccaccgaaugccuaagguuuccugaggaaggcucguccgcucagg
guuagucgggaccuaagccgaggccgaaaggcguaggcgauggacaacag
guugauauuccuguaccaccuccucaccauuugagcaauggggggacgca
ggaggauaggguaagcgcgguauuggauauccgcguccaagcaguuaggc
ugggaaauaggcaaauccguuucccaaaggcugagcugugauggcgagcg
cgaaguuccugauuccacacugccaagaaaagccucuagcgaggugagag
gugcccguaccgcaaaccgacacagguaggcgaggagagaauccuaaggu
gaucgagagaacucucguuaaggaacucggcaaaaugaccccguaacuuc
gggagaaggggugcucuguuagggugcaagcccgagagagccgcagugaa
uaggcccaggcgacuguuuagcaaaaacacaggucucugcgaagccguaa
ggcgaaguauaggggcugacgccugcccggugcuggaagguuaagaggag
cgcuuagcguaagcgaaggugcgaauugaagccccaguaaacggcggccg
uaacuauaacgguccuaagguagcgaaauuccuugucggguaaguuccga
cccgcacgaaaggcgcaacgaucugggcacugucucaacgagagacucgg
ugaaauuauaguaccugugaagaugcagguuacccgcgacaggacggaaa
gaccccguggagcuuuacugcagccugauauugaauguugguacagcuug
uacaggauagguaggagccuuggaaaccggagcgccagcuucgguggagg
caucggugggauacuacccuggcuguauugaccuucuaacccgccgcccu
uaucgggcggggagacagugucaggugggcaguuugacuggggcggucgc
cuccuaaaagguaacggaggcgcccaaagguucccucagaaugguuggaa
aucauucgcagaguguaaaggcacaagggagcuugacugcgagaccuaca
agucgagcagggacgaaagucgggcuuagugauccggugguuccgcaugg
aagggccaucgcucaacggauaaaagcuaccccggggauaacaggcuuau
cucccccaagaguccacaucgacggggagguuuggcaccucgaugucggc
ucaucgcauccuggggcuguagucggucccaaggguugggcuguucgccc
auuaaagcgguacgcgagcuggguucagaacgucgugagacaguucgguc
ccuauccgucgcgggcgcaggaaauuugagaggagcuguccuuaguacga
gaggaccgggauggacgcaccgcugguguaccaguuguucugccaagggc
aucgcuggguagcuaugugcggacgggauaagugcugaaagcaucuaagc
augaagccccccucaagaugagauuucccauuccgcaaggaaguaagauc
ccugaaagaugaucagguugauaggucugagguggaaguguggcgacaca
uggagcugacagauacuaaucgaucgaggacuuaacc

<<<<<<<......<<<<<<<<<<<.....[[.(((<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<.<<<<<<<<<.<<<.....<<<<......>>>>.((......
<<<.....))>>>...>>>...>>>>>>>>>.>>>>..>>>>.....<<<
<<<<<.........>>>>>>>>......<<<<<..<<<<>>>>>..))).
.....]].>>>>>>>.)))..............<<....>>....]]...
......<<<<....>>>>..................>>>>>>>>>>>...
....<<<<..<<<<......>>>>..>>>>...<.<........>.>.<<
<<<<....<<<<<<<.<<<<<..<<<...<<<.....>>>...>>>...<
<....>>..<<<<.....>>>>......>>>>>.>>>>>>>..<<.<...
<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>>>>...
..>>>...<<<.<<<<<........>>>>>>>>....>>>>>>>>>...<
<<.....>>>......>>>>>>......>.>>..<<<<<<....<<<<<.
...>>>>><<<<<<<<<...<<<<<<<<<.....<<<...<<<<<<<<..
.<<.<<<<.....>>>>..>>...>>>>>>>>..>>.>.....>>>>.>>
>>>..>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<<<<<<......<.<<<<<.<<...<<<<.......>>>>..
.>>.........<<......>>...>>>>>.>..>>>>>>>>>.>>....
.......))).....>>>>>>.>>>...>>>>>>><<<<<<..>>>>>>.
...>>>>>>..<<<<<<<.<<......<<<<<.<<....>>.>>>>>...
>>.>>>>>>>........>>>>>>.......<<....<<<<<<.......
>>>>>><<<<....<<<<<.<<<..<<<<<<...<<.....>>.>>>>>>
....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<
<........>>>>>))<<<<<.<<<<<<......<<...<<<<....<<<
<..<<<..<<<.<<<<<<<<...(((....>>>>>><<..<<<<<..<<<
<<<<<<<<.<<.....>>>>>>>>>...>>>>..>>>>>...>><..<..
>...>>>>>>...>>>...>>>>.........>>>>..>>..>>>>>>>>
>>>>>>>.>>>.....<<......>>>>>>>.<<<<.....>>>>..>>>
>..<<<<....<<<<<<<<.<<...............<<<<<....<...
.>.....>>>>><<<<<....<<<<....>>>>...>>>>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....
>>>.......>>>>>>>..<<<<.<<<..<<<<<<<..<<<...<<...<
<<<<<.<<....>>..>>>>>>...>>...>>>>>>>>..>>>>><<<<<
<.......>>>>>>........<...........<<<<<.......>>>>
>.....>....>>>>...>>.>>>>>>>.....>>.>>>>>>>>.>>>>>
>...........<<<<<........>>>>>..<<<<<<<<.(((.[[...
...<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<<<<<<<<.....
..........<<<<..<<<......<<<<<<<<....>>>>>>>>...>>
>............>>>>......>>>>>>>>>.>>>....<<.<<<<<<.
....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>><<
<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<......
.>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<.....
.>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>>>
..))>>>>>>>.............>>>>..]]>>>>.......<<<<...
<<<<<<...<<.......>>...>>>>>>....>>>.>.......<<<<<
.<.<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>
>.....>>>>...>...>>>.>>....<<<<<<<....>>>.>>>>....
]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<........
.......>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.)))>>>>>
>..>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>
>>....>>>.>>>>.......>>>>>>>..<<<<....>>>>....<<<<
<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>
>...>>>..>>>>>.>>>...>>>>....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ha1 Structural basis for antibiotic resistance mediated by theBacillus subtilisABCF ATPase VmlR.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): S2 Y3 R4 K5 G7 R8 S10 Q12 R13 A15 R18 L20 D23 I30 E31 T32 T33 T35 R36 K38 E39 R41 S42 E45 K46 T49 R53 L56 H57 R59 R60 Q61 A64 R67 N68 E69 Q81 Q95 G96 G97 K103 R107 R108 G109 D110
• Binding residue (residue number reindexed from 1): S1 Y2 R3 K4 G6 R7 S9 Q11 R12 A14 R17 L19 D22 I29 E30 T31 T32 T34 R35 K37 E38 R40 S41 E44 K45 T48 R52 L55 H56 R58 R59 Q60 A63 R66 N67 E68 Q80 Q94 G95 G96 K102 R106 R107 G108 D109

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ha1, PDBe:6ha1, PDBj:6ha1
• PDBsum: 6ha1
• PubMed: 30126986
• UniProt: P20277|RL17_BACSU Large ribosomal subunit protein bL17 (Gene Name=rplQ)