Structure of PDB 7r4f Chain M Binding Site BS01

Receptor Information

>7r4f Chain M (length=453) Species: 9913 (Bos taurus)

MLKYIIPTIMLMPLTWLSKNNMIWVNSTAHSLLISFTSLLLMNQFGDNSL
NFSLLFFSDSLSTPLLILTMWLLPLMLMASQHHLSKENLTRKKLFITMLI
SLQLFLIMTFTAMELILFYILFEATLVPTLIIITRWGNQGLYFLFYTLAG
SLPLLVALIYIQNTVGSLNFLMLQYWVQPVHNSWSNVFMWLACMMAFMVK
MPLYGLHLWLPKAHVEAPIAGSMVLAAVLLKLGGYGMLRITLILNPMTDF
MAYPFIMLSLWGMIMTSSICLRQTDLKSLIAYSSVSHMALVIVAILIQTP
WSYMGATALMIAHGLTSSMLFCLANSNYERIHSRTMILARGLQTLLPLMA
TWWLLASLTNLALPPTINLIGELFVVMSTFSWSNITIILMGVNMVITALY
SLYMLIMTQRGKYTYHINNISPSFTRENALMSLHILPLLLLTLNPKIILG
PLY

Ligand information

>7r4f Chain Y (length=20) Species: 9913 (Bos taurus)

AALVKMGQLEGWQVFAEPKV

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r4f Structural basis of mammalian respiratory complex I inhibition by medicinal biguanides.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): W190 S191 F194
• Binding residue (residue number reindexed from 1): W184 S185 F188

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0048039 ubiquinone binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:0009060 aerobic respiration
• GO:0015990 electron transport coupled proton transport
• GO:0032981 mitochondrial respiratory chain complex I assembly
• GO:0042773 ATP synthesis coupled electron transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:7r4f, PDBe:7r4f, PDBj:7r4f
• PDBsum: 7r4f
• PubMed: 36701435
• UniProt: P03910|NU4M_BOVIN NADH-ubiquinone oxidoreductase chain 4 (Gene Name=MT-ND4)