Structure of PDB 8y0u Chain LZ Binding Site BS01

Receptor Information

>8y0u Chain LZ (length=135) Species: 4932 (Saccharomyces cerevisiae)

AKFLKAGKVAVVVRGRYAGKKVVIVKPHDEGSKSHPFGHALVAGIERYPL
KVTKKHGAKKVAKRTKIKPFIKVVNYNHLLPTRYTLDVEAFKSVVSTETF
EQPSQREEAKKVVKKAFEERHQAGKNQWFFSKLRF

Ligand information

>8y0u Chain C1 (length=3221)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacgccagcaucaguuuugguggcaggauaaauccauaggaauguag
cuugccucgguaaguauuauagccugugggaauacugccagcugggacug
aggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgccc
gucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugggug
uaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaaga
ggugcacaaucgaccgauccugaugucuucggauggauuugaguaagagc
auagcuguugggacccgaaagauggugaacuaugccugaauagggugaag
ccagaggaaacucugguggaggcucguagcgguucugacgugcaaaucga
ucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuaguagc
ugguuccugccgaaguuucccucaggauagcagaagcucguaucaguuuu
augagguaaagcgaaugauuagagguuccggggucgaaaugaccuugacc
uauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaacgu
ggacauuugaaugaagagcuuuuagugggccauuuuugguaagcagaacu
ggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacacgcu
caucagacaccacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaaaauggauggcgcucaagcguguuaccuauacucuac
cgucaggguugauaugaugcccugacgaguaggcaggcguggaggucagu
gacgaagccuagaccguaaggucgggucgaacggccucuagugcagaucu
uggugguaguagcaaauauucaaaugagaacuuugaagacugaagugggg
aaagguuccacgucaacagcaguuggacguggguuagucgauccuaagag
auggggaagcuccguuucaaaggccugauuuuaugcaggccaccaucgaa
agggaauccgguuaagauuccggaaccuggauauggauucuucacgguaa
cguaacugaauguggagacgucggcgcgagcccugggaggaguuaucuuu
ucuucuuaacagcuuaucaccccggaauugguuuauccggagaugggguc
uuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugugac
ggcccgugaaaauccacaggaaggaauaguuuucaugccaggucguacug
auaaccgcagcaggucuccaaggugaacagccucuaguugauagaauaau
guagauaagggaagucggcaaaauagauccguaacuucgggauaaggauu
ggcucuaagggucggguacgaucaacuuagaacugguacggacaagggga
aucugacugucuaauuaaaacauagcauugcgauggucagaaagugaugu
ugacgcaaugugauuucugcccagugcucugaaugucaaagugaagaaau
ucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaaggua
gccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaacgag
auucccacugucccuaucuacuaucuagcgaaaccacagccaagggaacg
ggcuuggcagaaucagcggggaaagaagacccuguugagcuugacucuag
uuugacauugugaagagacauagaggguguagaauaagugggagcuucgg
cgccagugaaauaccacuaccuuuauaguuucuuuacuuauucaaugaag
cggagcuggaauucauuuuccacguucuagcauucaaggucccauucggg
gcugauccggguugaagacauugucagguggggaguuuggcuggggcggc
acaucuguuaaacgauaacgcagauguccuaaggggggcucauggagaac
agaaaucuccaguagaacaaaaggguaaaagcccccuugauuuugauuuu
cagugugaauacaaaccaugaaaguguggccuaucgauccuuuagucccu
cggaauuugaggcuagaggugccagaaaaguuaccacagggauaacuggc
uuguggcagucaagcguucauagcgacauugcuuuuugauucuucgaugu
cggcucuuccuaucauaccgaagcagaauucgguaagcguuggauuguuc
acccacuaauagggaacgugagcuggguuuagaccgucgugagacagguu
aguuuuacccuacugaugaauguuaccgcaauaguaauugaacuuaguac
gagaggaacaguucauucggauaauugguuuuugcggcugucugaucagg
cauugccgcgaagcuaccauccgcuggauuauggcugaacgccucuaagu
cagaauccaugcuagaacgcggugauuucuuugcuccacacaauauagau
ggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggcuagc
aacggugcacuuggcggaaaggccuugggugcuugcuggcgaauugcaau
gucauuuugcguggggauaaaucauuuguauacgacuuagauguacaacg
ggguauuguaagcaguagaguagccuuguuguuacgaucugcugagauua
agccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>...{.....<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]....}..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<<................
......<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<<......<
<<<<<....>>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>>>>
........<<<....>>>.....>>>>>>>....>>>>>>.>>>>>..<<
<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<.
....>>>>...>>>...<<....>>.......<<<<<..<<<<<....>>
>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....>>
>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<
<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>>>
.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>..>
.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<<<<
<<<.....<<<....<<<..<.<<<<<..<<<<<<.....>>>>>>>>>>
>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.
>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>
>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.
....<<..........<<<.<<<<<<<<<<.......<<<<<.<<<...<
<.........>>...>>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>>>
<<<<<<<<<.........>>>>>>>>>....>>>>>><<<<<<<<<<.<<
......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.>>.....>>
>>>><<<....<<.....<<<<<<.......>>>>>><<<<.....<<<<
<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<.
...<<<....>>>........<<<<<<<.....>.>>>>>>....>>...
.>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<....
>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{..>
>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>>.
....>>>>>....><<<<<<<......>>>>>>>>>>>...>>>>>>>.>
>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>>.
....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....
<<<<<<<.<<.........<.....<<<<<....<....>.....>>>>>
<<.<<<<<<<<<<<....>>>>>..>>>>>>.>>............<<<<
<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>>>..
....>>>>>>>...<<..<<<..<<<<<<.....<<....<<<<<....>
>>>.>...>>..>>>>..>>>>><<<<<<.......>>>>>>........
<...........<<<<<.......>>>>>.....>.....>>....>>.>
>>>>>>..>..>>.>>>>>>>..>>>>>>...>>>....<<<<<......
..>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<
<<<<<<<..<<<<<<<<<<<<<<<<<..........<<.<..........
..............>.>>.>>>>>>>.>>>>>>>>>>...<<<<<.....
<<<<..<<<<<......>>>>>...............<<<<<<....>>>
>>>..>>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>
..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<..
.....>>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.
......>>>>.>>>>.<<<....>>>.>>))).......((((((..(((
(.>>>>>>))))))))))..........]]]]..}}]]]].......<<.
<.<.<<<<<<...<<.......>>...>>>>>>...>>..>>.......<
<<<<.<<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.......
..>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>
....}}..}}}.]]]]]....<<<<<<<<..<<<<.<.<<<<<<......
.........>>>>>>.><<<<<...<<<<...<<<<<<<<<<<}}}.>>>
>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<...........>>>
>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<....
<<..........<<<<<<.....>>>>>>......>>.......<<<<<<
...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>..........
...>>.>>>.>>>>>>..<<<<<.<.............>.....<<<<<<
<<<.<<<....<<<<....<<<<<......>>>>>...>>>>...>>>..
..>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8y0u dormant ribosome with STM1
• Resolution: 3.59 Å
• Binding residue (original residue number in PDB): G16 R17 H36 F38 T54 K55 G58 K60 K64 K67 K69 K73 N76 H79 R107 K133 R135 F136
• Binding residue (residue number reindexed from 1): G15 R16 H35 F37 T53 K54 G57 K59 K63 K66 K68 K72 N75 H78 R106 K132 R134 F135

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8y0u, PDBe:8y0u, PDBj:8y0u
• PDBsum: 8y0u
• PubMed: 38698775
• UniProt: P0C2H6|RL27A_YEAST Large ribosomal subunit protein eL27A (Gene Name=RPL27A)