Structure of PDB 1gcz Chain B Binding Site BS01

PDB	1gcz Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography.
Resolution	1.9 Å
Binding residue (original residue number in PDB)	P1 M2 K32 H62 S63 I64 V106 F113
Binding residue (residue number reindexed from 1)	P1 M2 K32 H62 S63 I64 V106 F113
Annotation score	1
Binding affinity	MOAD: Ki=7.4uM BindingDB: Ki=7400nM

Enzymatic activity

Enzyme Commision number

5.3.2.1: phenylpyruvate tautomerase.
5.3.3.12: L-dopachrome isomerase.

Gene Ontology

	Molecular Function
GO:0002020	protease binding
GO:0004167	dopachrome isomerase activity
GO:0005125	cytokine activity
GO:0005126	cytokine receptor binding
GO:0005515	protein binding
GO:0016853	isomerase activity
GO:0042056	chemoattractant activity
GO:0042802	identical protein binding
GO:0050178	phenylpyruvate tautomerase activity

	Biological Process
GO:0001516	prostaglandin biosynthetic process
GO:0001819	positive regulation of cytokine production
GO:0001934	positive regulation of protein phosphorylation
GO:0002906	negative regulation of mature B cell apoptotic process
GO:0006954	inflammatory response
GO:0007166	cell surface receptor signaling pathway
GO:0008284	positive regulation of cell population proliferation
GO:0010629	negative regulation of gene expression
GO:0010760	negative regulation of macrophage chemotaxis
GO:0019752	carboxylic acid metabolic process
GO:0030330	DNA damage response, signal transduction by p53 class mediator
GO:0030336	negative regulation of cell migration
GO:0030890	positive regulation of B cell proliferation
GO:0031666	positive regulation of lipopolysaccharide-mediated signaling pathway
GO:0032760	positive regulation of tumor necrosis factor production
GO:0033033	negative regulation of myeloid cell apoptotic process
GO:0042127	regulation of cell population proliferation
GO:0042327	positive regulation of phosphorylation
GO:0043030	regulation of macrophage activation
GO:0043066	negative regulation of apoptotic process
GO:0043406	positive regulation of MAP kinase activity
GO:0043518	negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045087	innate immune response
GO:0048146	positive regulation of fibroblast proliferation
GO:0050918	positive chemotaxis
GO:0051248	negative regulation of protein metabolic process
GO:0061078	positive regulation of prostaglandin secretion involved in immune response
GO:0061081	positive regulation of myeloid leukocyte cytokine production involved in immune response
GO:0070207	protein homotrimerization
GO:0070374	positive regulation of ERK1 and ERK2 cascade
GO:0090238	positive regulation of arachidonate secretion
GO:0090398	cellular senescence
GO:0141163	positive regulation of cAMP/PKA signal transduction
GO:1902166	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:2000343	positive regulation of chemokine (C-X-C motif) ligand 2 production
GO:2000773	negative regulation of cellular senescence

	Cellular Component
GO:0005576	extracellular region
GO:0005615	extracellular space
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0009986	cell surface
GO:0031982	vesicle
GO:0034774	secretory granule lumen
GO:0070062	extracellular exosome
GO:1904813	ficolin-1-rich granule lumen

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1gcz, PDBe:1gcz, PDBj:1gcz
PDBsum	1gcz
PubMed	11170644
UniProt	P14174\|MIF_HUMAN Macrophage migration inhibitory factor (Gene Name=MIF)

[Back to BioLiP]