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Regarding Modelling of Pentamer Nanobody
Posted: Thu Oct 26, 2023 12:30 pm
by Ahbab Ali
Good evening sir\ma'am
I hope this message finds you well. Currently, I am working on a homology modeling of a homo-pentameric nanobody project which is part of my dissertation. My query is, can I make a homo-pentamer of a Nanobody, for which templates are not available on PDB by the I-TASSER web server? if yes! then, please share the protocol or steps it would be helpful for me.
Thank you
Ahbab Ali
M.S.(Pharm.) Biotechnology
National Institute of Pharmaceutical Education and Research
Re: Regarding Modelling of Pentamer Nanobody
Posted: Thu Oct 26, 2023 11:45 pm
by jlspzw
Dear user,
You can try our most cutting-edge method
https://zhanggroup.org/D-I-TASSER/
Best
IT Team
Re: Regarding Modelling of Pentamer Nanobody
Posted: Wed Jul 02, 2025 4:16 pm
by peoplebreast
Ahbab Ali wrote: ↑Thu Oct 26, 2023 12:30 pm
Good evening sir\ma'am
I hope this message finds you well. Currently, I am working on a homology modeling of a homo-pentameric nanobody project which is part of my dissertation. My query is, can I make a homo-pentamer of a Nanobody, for which templates are not available on PDB by the I-TASSER web server? if yes! then, please share the protocol or steps it would be helpful for me.
Thank you
Ahbab Ali
M.S.(Pharm.) Biotechnology
National Institute of Pharmaceutical Education and Research
Yes, I-TASSER models only monomeric structures. For a homo-pentamer nanobody, first use I-TASSER to model the monomer, then use tools like SymmDock, ClusPro, or Rosetta SymDock to assemble the pentamer based on symmetry.
Re: Regarding Modelling of Pentamer Nanobody
Posted: Thu Jul 24, 2025 4:21 pm
by Enochflyn
peoplebreast wrote: ↑Wed Jul 02, 2025 4:16 pm
Ahbab Ali wrote: ↑Thu Oct 26, 2023 12:30 pm
Good evening sir\ma'am
I hope this message finds you well. Currently, I am working on a homology modeling of a homo-pentameric nanobody project which is part of my dissertation.
papa's scooperia My query is, can I make a homo-pentamer of a Nanobody, for which templates are not available on PDB by the I-TASSER web server? if yes! then, please share the protocol or steps it would be helpful for me.
Thank you
Ahbab Ali
M.S.(Pharm.) Biotechnology
National Institute of Pharmaceutical Education and Research
Yes, I-TASSER models only monomeric structures. For a homo-pentamer nanobody, first use I-TASSER to model the monomer, then use tools like SymmDock, ClusPro, or Rosetta SymDock to assemble the pentamer based on symmetry.
I agree with you