Help with CRISPR Base Editing Efficiency in Cell Cultures

[JacquelineJCastle]

Hi all,
The efficiency of CRISPR base editing has presented challenges that researchers face in achieving desired mutations in cell cultures with remarkable success rates.poppy playtime chapter 3

[Scott324]

To improve CRISPR base editing efficiency, consider optimizing your sgRNA design and testing different transfection reagents or methods. Timing is crucial, so experiment with cell cycle phases and post-transfection analysis times. Additionally, using selection markers and co-delivering factors like repair templates can enhance success rates. Good luck!cookie clicker

[pigkang]

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