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COFACTOR result for job id 76463

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>76463 (433 residues)
MKFVDFADLIIKAGKGGDGAVSFLHALFVPNGGPNGGDGGDGGSVYFLGDEGKHSLLDLK
LQKKYSAQDGFKGDIKNMHGAKGEDKIIKVPVGTILYDKKTNTILADINENNKLVLIAKG
GKGGKGNARFANSRNKAPTIFEAGELGQEFEIRAELKVLADVGFVGLPNAGKSTLLRAIS
NSKPVVADYPFTTITPQLGVARTKNNDTFIVADLPGLIQGASLGKGLGHQFLKHIERCLV
ICHIIDASGNFGSEDIIKNYELIRDELKAYNLNLEKRPEIIVLNKMDLDEAQLNLLDEKI
INYFKDKKVVRISGLKKENIDQLLFMIYEELKVAKKQPLWELDKNNDQDEIAIYKFEEQK
EDIQVYNKGNNRWEIAGETIFKIYQKFPIWTEDNLLMFNEKLKETGVYETLVKKGIKKGD
FVKVFDYELEWTD

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1udxA	0.94	0.69	0.371	0.947	Download
2	1lnzA	0.40	4.11	0.279	0.471	Download
3	4csu9	0.40	5.22	0.250	0.533	Download
4	5ca8A	0.40	5.61	0.099	0.547	Download
5	6ez8A	0.39	6.99	0.066	0.635	Download
6	2e87A	0.39	4.70	0.140	0.485	Download
7	5gnrA	0.39	5.83	0.068	0.547	Download
8	6ar6A	0.38	7.00	0.046	0.619	Download
9	2hjgA	0.37	4.97	0.121	0.473	Download
10	5kcaA	0.37	5.88	0.038	0.522	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.88	catalytic activity
GO:1901363	0.82	heterocyclic compound binding
GO:0097159	0.82	organic cyclic compound binding
GO:0016787	0.81	hydrolase activity
GO:0017111	0.78	nucleoside-triphosphatase activity
GO:0036094	0.75	small molecule binding
GO:0035639	0.74	purine ribonucleoside triphosphate binding
GO:0032555	0.74	purine ribonucleotide binding
GO:0032550	0.73	purine ribonucleoside binding
GO:0003924	0.73	GTPase activity
GO:0005525	0.72	GTP binding
GO:0043167	0.52	ion binding

Download full result of the above consensus prediction.

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(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.93	cellular process
GO:0071704	0.75	organic substance metabolic process
GO:0071840	0.74	cellular component organization or biogenesis
GO:0044237	0.74	cellular metabolic process
GO:0044238	0.72	primary metabolic process
GO:0006807	0.69	nitrogen compound metabolic process
GO:0034641	0.66	cellular nitrogen compound metabolic process
GO:0043170	0.62	macromolecule metabolic process
GO:0016043	0.53	cellular component organization
GO:0044260	0.51	cellular macromolecule metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.89	cytoplasmic part
GO:0005737	0.84	cytoplasm
GO:0043226	0.68	organelle
GO:0043229	0.67	intracellular organelle
GO:0044446	0.61	intracellular organelle part
GO:0032991	0.56	macromolecular complex
GO:0043227	0.52	membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1mo7A	0.184	5.92	0.045	0.270	3.6.3.9	NA
2	0.060	1d2eA	0.328	3.76	0.107	0.386	3.6.5.3	NA
3	0.060	2gksB	0.321	6.53	0.099	0.487	2.7.7.4 2.7.1.25	NA
4	0.060	2iufA	0.284	7.22	0.047	0.483	1.11.1.6	NA
5	0.060	1xzpA	0.321	5.06	0.117	0.423	3.6.5.-	74

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.06	1lnzA	0.411	4.18	0.277	0.483	0.66	MG	Download	18,19,38,39,40
2	0.01	3aruA	0.354	6.31	0.053	0.533	0.42	PNX	Download	40,41,42,68,69
3	0.01	1mneA	0.350	6.84	0.049	0.566	0.49	POP	Download	43,69,70,85,120,121
4	0.01	3as0A	0.351	6.29	0.056	0.529	0.62	SAU	Download	42,43,44,67

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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