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I-TASSER results for job id S802700

(Click on S802700_results.tar.bz2 to download the tarball file including all modeling results listed on this page. Click on Annotation of I-TASSER Output to read the instructions for how to interpret the results on this page. Model results are kept on the server for 60 days, there is no way to retrieve the modeling data older than 2 months)

  Submitted Sequence in FASTA format

>protein
MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGGPDSCPVVLHSFTQLDPDLPRL
ESSTQEIGEELVNGVVYSISLRKVQVHHGATKGQRWLGCENESALNLYETCKVRTVKAGT
LEKLVGHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFERYGCILPYAHEDGGPQDQ
LKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHAE
LTQAEPEALKPAPELELAPAPLLAPSPVPAPDLEPVPGPDLEPALAPAPDPEPAPAPAPT
PELELALSQSLELEPASAPEPSWPSPVAAEKGLGEEKPHLLAFPAVLVAEQFTLMDAELF
KKVVPYHCLGSIWSQRDKKGKEHLAPTVRATVTQFNNVANCVITTCLGDRSVTARGRARV
VEHWIQVARECRALKNFSSLYAILSALQSNSIHRLKKTWDEVSRESFRIFQKLSEIFSDE
NNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGVIQGTVPYLGTFLTDLVMLDTAMK
DYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEQFRAWFQAMEWLSETESYNLS
CELEPPSESASNTLRVKKNAAIVKRWSDRQAPSTELSASSSCHSKSCDQLRCSPYLSSGD
IADALSVHSAGSSSSDVEEINVSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSS
SASTTPVATARTHKRSVSGVCSHGSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQ
DKAPAVIRKAMDKHNLDEDEPEDYELVQVISEDRKLKIPDNANVFYAMNSTANYDFVLKK
RTFTKGAKVRHGASSTLPRMKQKGLKIAKGIF

  Predicted Secondary Structure

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860
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MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGGPDSCPVVLHSFTQLDPDLPRLESSTQEIGEELVNGVVYSISLRKVQVHHGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVGHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFERYGCILPYAHEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHAELTQAEPEALKPAPELELAPAPLLAPSPVPAPDLEPVPGPDLEPALAPAPDPEPAPAPAPTPELELALSQSLELEPASAPEPSWPSPVAAEKGLGEEKPHLLAFPAVLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTVRATVTQFNNVANCVITTCLGDRSVTARGRARVVEHWIQVARECRALKNFSSLYAILSALQSNSIHRLKKTWDEVSRESFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGVIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEQFRAWFQAMEWLSETESYNLSCELEPPSESASNTLRVKKNAAIVKRWSDRQAPSTELSASSSCHSKSCDQLRCSPYLSSGDIADALSVHSAGSSSSDVEEINVSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVATARTHKRSVSGVCSHGSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLDEDEPEDYELVQVISEDRKLKIPDNANVFYAMNSTANYDFVLKKRTFTKGAKVRHGASSTLPRMKQKGLKIAKGIF
PredictionCCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCSSSCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSSCCCCCCCSSSSSSSCCCCCCHHHHHHHHHHCCCCCCCHHHCSSSSSCCCCCSSCCCCCCCSSSSSCCCCCCCSSSSCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHCC
Conf.Score91034222048898888777655443011134543357877788676442304776440344554421232378210475244466788886645455787510110454776607644899999982533446666599999986667488799999999986225874220166776532010019999998604441230236889999987664044523677888767653213322110000023432123455311346643222233234221121124344444655554223334543000034334666420011222124333545021478899999999999999868999998666642566667766795399999999999999856304689988999999999999999999986798899999998547620635999997897889999999974384003999999986532455641002414555554303588874264999999998876128765788751599999999999999998525888898666999999972889999999998886279998888877765652000232101477656676777667775445678777777766667777766788764446667688887766567776666789877887877777888988788878776678777777678888875556788701465322577775345567503343629999999974688557767556676699873217888622366447776216984256776667467877653656664130544229
H:Helix; S:Strand; C:Coil

  Predicted Solvent Accessibility

Sequence                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860
                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |            
MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGGPDSCPVVLHSFTQLDPDLPRLESSTQEIGEELVNGVVYSISLRKVQVHHGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVGHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFERYGCILPYAHEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHAELTQAEPEALKPAPELELAPAPLLAPSPVPAPDLEPVPGPDLEPALAPAPDPEPAPAPAPTPELELALSQSLELEPASAPEPSWPSPVAAEKGLGEEKPHLLAFPAVLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTVRATVTQFNNVANCVITTCLGDRSVTARGRARVVEHWIQVARECRALKNFSSLYAILSALQSNSIHRLKKTWDEVSRESFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGVIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEQFRAWFQAMEWLSETESYNLSCELEPPSESASNTLRVKKNAAIVKRWSDRQAPSTELSASSSCHSKSCDQLRCSPYLSSGDIADALSVHSAGSSSSDVEEINVSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVATARTHKRSVSGVCSHGSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLDEDEPEDYELVQVISEDRKLKIPDNANVFYAMNSTANYDFVLKKRTFTKGAKVRHGASSTLPRMKQKGLKIAKGIF
Prediction63432244443342434322433333443244143425344322020343331345264234214403413454231201034243331344333233243443333243243220000003200220032343323200100000010001034003100311211132332233324323210120031003202441122111100220131112101222033212210221121122211122222122122121211112112213123233332332223333333333332233232323222223223112120212012444144331300312021001000100130023030310022003342364343202102000310020010001000337514233003002200300320240000000000000000000200330054026603510430260034641023003203533234233244334434433344514000000000101001000300233166310002002200300320330061134050432440240034042032630041026100344536634533564422441443334343343444333333432414333233434333433333243343443434333433443444334333322143332323332331333333132334343333232323321113354534412003030436423201001023443012002300320314473143010011036433020064000000001212000000214367424364443331330343114004225
Values range from 0 (buried residue) to 9 (highly exposed residue)

   Predicted normalized B-factor

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. Click here to read more about predicted normalized B-factor)


  Top 10 threading templates used by I-TASSER

(I-TASSER modeling starts from the structure templates identified by LOMETS from the PDB library. LOMETS is a meta-server threading approach containing multiple threading programs, where each threading program can generate tens of thousands of template alignments. I-TASSER only uses the templates of the highest significance in the threading alignments, the significance of which are measured by the Z-score, i.e. the difference between the raw and average scores in the unit of standard deviation. The templates in this section are the 10 best templates selected from the LOMETS threading programs. Usually, one template of the highest Z-score is selected from each threading program, where the threading programs are sorted by the average performance in the large-scale benchmark test experiments.)

Rank PDB
Hit		Iden1Iden2CovNorm.
Z-score		Download
Align.		                   20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860
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Sec.Str
Seq		CCCHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCSSSSSCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCSSSCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCSSSSSSSCCCCCCCSSSSSSSCCCCCCHHHHHHHHHHCCCCCCCHHHCSSSSSCCCCCSSCCCCCCCSSSSSCCCCCCCSSSSCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHCC
MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGGPDSCPVVLHSFTQLDPDLPRLESSTQEIGEELVNGVVYSISLRKVQVHHGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVGHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFERYGCILPYAHEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHAELTQAEPEALKPAPELELAPAPLLAPSPVPAPDLEPVPGPDLEPALAPAPDPEPAPAPAPTPELELALSQSLELEPASAPEPSWPSPVAAEKGLGEEKPHLLAFPAVLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTVRATVTQFNNVANCVITTCLGDRSVTARGRARVVEHWIQVARECRALKNFSSLYAILSALQSNSIHRLKKTWDEVSRESFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGVIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEQFRAWFQAMEWLSETESYNLSCELEPPSESASNTLRVKKNAAIVKRWSDRQAPSTELSASSSCHSKSCDQLRCSPYLSSGDIADALSVHSAGSSSSDVEEINVSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVATARTHKRSVSGVCSHGSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLDEDEPEDYELVQVISEDRKLKIPDNANVFYAMNSTANYDFVLKKRTFTKGAKVRHGASSTLPRMKQKGLKIAKGIF
13ksyA 0.19 0.17 0.63 1.87Download HGQPRLPGASNAEYRLKFMRKVQINDKDDTNEYKHEIILKDENSVIFSAKSAKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSADVYRFAEPDSEENIIFEPKAGIPIIKAGTVIKLIERLTYH-MYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGD---QPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDAYLLQRMEEFIGTVRGKAMKKWVESITKIIQ-------------------------RKKIARDNHNITFQSSPP-----------------------TVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEIN---SPNLLKMIRHTTNLTLWFEKCIVETEN--LEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELS--EDHYKKYLAKLRS------------------------INPPCVPFFGIYLTNILKTEEGNPEVLKKELINFSKRRKVAEITGEIQQYQNQ--PYCLRVESDIKRFFENLNPMGTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNP------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
27e2cJ 0.09 0.17 0.73 0.85Download --------------------------------------------------------------------------------------------------------------------------------------------------RSQQKSLGRQMKILGNFQLLAGRYVDALNSFVDA-----------------ITTLYKVRDYLLDGISICFLLLSYRNSISIAAMQSPRNSIMSLSAPALNIDVENINLPLLIKCISDKVLYYYDLSLMHNSEVYCEFLLKTLTFMTSCYKSSESKDVLDNIVKNQHRALSDIPNSPMFPRFEVYFYSNKLFELQLKEMQVEAQIKIYSTMAEVYRLLGLRLLMVALATPNKIAWHPDYRTLIDTIIEL------------LNINSTWLILQKKILQLCIKVSRKIN-------------------------DFEYVAKFSSILITKYTHLLNQSEQFKEYIQPSITNESITINRILDSDPTSNEIPAEFTCRVPFKFDFTI---------NDIQLDEEISFCEI-------------------DRKAVSYSGP---------YNVKAESIRSITLPLIIKKPTYKKIYEISKISILKLPLQKFDIINDSRRSNPVEEEAE-KMTRNSWMMLDGTKTDFHITVRNKSLSCAINHIKIIPMNNIEQMLKPDYWKKMPPDDLYIMEKQLSKSCVRIIKLITFDLELDNTAVPFNFTGFDLLIEYGMSATDESCIYLKKLSIPY-EVTLRRTIEVPSSQVENVDWIEYVMSKIRAESNLHSLLLDFRNSWIDGIKQFEDFTSNYHVEASSRIIVPIKKIDYKKTPIPRIYPGRQFIGLNEEQTIEMRQKF
34jgw 0.42 0.20 0.44 3.76Download ----------------------------------------------------------EEEASVSVWD-EEEDGATFTVTSR--QYRPLDPLAP------------LPPPRSSRRLRAGTLEALVRHLLDARAGADMMFTPALLATHRAFTSTPALFGLVADRLEALESYP---PGELERTTGVAISVLSTWLASHPEDFGSEV-KGQLDRLESFLLRTGYSADLIR-------NLRARD-------------------------------------------------------S-------------------------------------PADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLW----------LCPSVRATVTQFNKVAGAVVSSVLGATPLRPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFS--------RE----------------------------LLTGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKECRGYDLRPNSDIQQWLQGLQPLTEAQSHRVSCEVEP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
45ovd 0.22 0.15 0.51 2.60Download ------------------------------------------------------------------------------GAMAEEQMRLPSADVYRFAEPDSEENI-IFEE-GIPIIKAGTVIKLIERLTYHM-YADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADEYIQPVQLRVLNVCRHWVEHHFYDFERDA--YLLQRMEEFIGTVRGK-AMK-KWVESITKIIQRKKIAR---------------------------TF------------Q----S--SPPTVE---------------------------WHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKE---INSPNLLKMIRHTTNLTLWFEKCIVETEN--LEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHEL--SEDHYKKYLAKLR------------------------SINPPCVPFFGIYLTNILKTEEGNPEVLKKELINFSKRRKVAEITGEIQQYQNQ--PYCLRVESDIKRFFENLNPMMTDYLFNKSLEIEPRNPKPLPRFPKKYSY---PLKSPGVRPS------N----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
53ksyA 0.20 0.15 0.53 2.43Download -------------------------------------------------------------------------------------MRLPSADVYRFAEPDSEENIIFEENAGIPIIKAGTVIKLIERLTYH-MYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGDQP---LSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDAYLLQRMEEFIGTV------------------------------------------------RGKAMKKWVESITKIIQRKKIARDNHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKED---KEINSPNLLKMIRHTTNLTLWFEKCIVETE--NLEERVAVVSRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHEL--SEDHYKKYLAKLRS------------------------INPPCVPFFGIYLTNILKTEEGNPEVLKRHLINFSKRRKVAEITGEIQQYQN--QPYCLRVESDIKRFFENLNPMGTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNP------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
64jgw 0.42 0.20 0.44 4.15Download -----------------------------------------------------------EEASVSVWD-EEEDGATFTVTSR--QYRPLDPLAPLP------------PPRSSRRLRAGTLEALVRHLLDARAGADMMFTPALLATHRAFTSTPALFGLVADRLEALESYP---PGELERTTGVAISVLSTWLASHPEDFGSEV-KGQLDRLESFLLRTGYSADLIR-------NLRARD--------------------------------------------------------------------------------S------------PADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLW----------LCPSVRATVTQFNKVAGAVVSSVLGATPLRPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFS--------RELL----------------------------TGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKECRGYDLRPNSDIQQWLQGLQPLTEAQSHRVSCEVEP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
74f7zA 0.17 0.17 0.63 4.40Download LLSQMGPDAHMRMILRKPPGQRTVDDLEIIYDELLHIKALSHLS--------------------TTVKRELAGVLIFESHAKGNDAPRAAEDGNRILRDVEANTVRLKEHDKVYTVMSGTPEKILEHFLETIRLEPDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGQERMDYALNNKRRVIRLVLQWAAMYGDLLQEDVAMAFLEEFYVSVSDDARMMAAFKEQLPELEKIVKQISEDAKAPQKKHKRAQKRVPVAASVKEVISAVADKLGSGEGLIIVKMNSKSNDVSVFTTLTINGRLFACPREQFDSLPLPEQEGPTT--------GTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFG---RHNFKKTTANLDLFLRRFNEIQFWVVTEVCLCSQ--LSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAK------------------------LEPPLIPFMPLLIKDMTFTHEGNKTFI-DNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQKQDVRSYVRQLNVINQRTLSQMSHRLEPR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
87nzzA 0.23 0.14 0.47 2.19Download -------------------------------------------------------------------------------------------DVPWYLEGDDEYELLLDVKGN---IKGGSKEALVSHLTHH-LSLDSNFNAVFLLMFSSMMSLGELISLLIARFNIEPPEGLSYEEYNLWVSKKRNPIMKLLLEKNWSMSYYNEPVLRRWLTFAHSDQVQTYSLGNLLVNYLERLL-------------------------------------------------------------------------RGERIRDPVIPNTKPPAPLTKGSSLSKKPRVMDIDYVELARQLTLREFKLYCKITKFACLAKVWGKKSGLS--ESIDSITQFIKASNQLTNFVGYMILRKAD--PKKRVQIIRYFIQVADKCRQYNNFSSMTAIISALYSSPIHRLKKTWEYMNADALSNLKNMNKLMNSSRNFNEYRDVL-----------------------KFIGSEPCVPFFGVYLSDLTFVYHGNPDYLRTRQVNFAKRAKTSEIVSGIDRFKTT--GYNFQEVPEIQKFLDAWKCPTIDEQYQISLNLEPRE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
95cm8A 0.41 0.21 0.47 6.71Download ---------------------------------------------------------------SVSVWDEEEDGATFTVTSRQYRPLDPLAPLP--------------PPRSSRRLRAGTLEALVRHLLARTAGADMMFTPALLATHRAFTSTPALFGLVADRLEALESYP---PGELERTTGVAISVLSTWLASHPED-FGSEVKGQLDRLESFLLRTGYAGSADLIRNLR---------------------------------------------------------------------------------------------------ARPADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGHSHLCPSVRATVTQFNKVAGAVVSSVLGVRPLRPPQRARLLEKWIRVAEECRLLRNFSSVYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSRELLTRS---------------------GFRGGGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKECRGYDLRPNSDIQQWLQGLQPLTEAQSHRVSCEVEPPG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
107scwB 0.70 0.07 0.10 2.94Download -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTCIIRISVEDNNGNMYKSIMLTSQDKTPAVIQRAMLKHNLDSDPAEEYELVQVISEDKELVIPDSANVFYAMNSQVNFDFILRKK-------------------------------
(a)All the residues are colored in black; however, those residues in template which are identical to the residue in the query sequence are highlighted in color. Coloring scheme is based on the property of amino acids, where polar are brightly coloured while non-polar residues are colored in dark shade. (more about the colors used)
(b)Rank of templates represents the top ten threading templates used by I-TASSER.
(c)Ident1 is the percentage sequence identity of the templates in the threading aligned region with the query sequence.
(d)Ident2 is the percentage sequence identity of the whole template chains with query sequence.
(e)Cov represents the coverage of the threading alignment and is equal to the number of aligned residues divided by the length of query protein.
(f)Norm. Z-score is the normalized Z-score of the threading alignments. Alignment with a Normalized Z-score >1 mean a good alignment and vice versa.
(g)Download Align. provides the 3D structure of the aligned regions of the threading templates.
(h)The top 10 alignments reported above (in order of their ranking) are from the following threading programs:
       1: MUSTER   2: SPARKS-X   3: HHSEARCH2   4: HHSEARCH I   5: Neff-PPAS   6: HHSEARCH   7: pGenTHREADER   8: PROSPECT2   9: SP3   10: FFAS03   

   Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of a higher value signifies a model with a higher confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated; this is usually an indication that the models have a good quality because of the converged simulations.)
    (By right-click on the images, you can export image file or change the configurations, e.g. modifying the background color or stopping the spin of your models)
  • Download Model 1
  • C-score=-2.84 (Read more about C-score)
  • Estimated TM-score = 0.39±0.13
  • Estimated RMSD = 15.9±3.2Å

  • Download Model 2
  • C-score = -3.55

  • Download Model 3
  • C-score = -3.79

  • Download Model 4
  • C-score = -3.78

  • Download Model 5
  • C-score = -3.95


  Proteins structurally close to the target in the PDB (as identified by TM-align)

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)


Top 10 Identified stuctural analogs in PDB

Click
to view		RankPDB HitTM-scoreRMSDaIDENaCovAlignment
17e2cJ0.659 3.080.0750.700Download
27wjiA0.384 8.060.0470.576Download
37sx3E0.382 8.470.0510.592Download
47r03A0.378 8.470.0440.584Download
55u1sA0.376 7.810.0550.548Download
66lk8A0.376 8.460.0460.579Download
77wkkA0.367 8.170.0470.558Download
85nvrA0.366 8.460.0410.567Download
97lheA0.365 7.920.0350.545Download
105hb4B0.356 7.920.0400.526Download

(a)Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.


  Predicted function using COFACTOR and COACH

(This section reports biological annotations of the target protein by COFACTOR and COACH based on the I-TASSER structure prediction. While COFACTOR deduces protein functions (ligand-binding sites, EC and GO) using structure comparison and protein-protein networks, COACH is a meta-server approach that combines multiple function annotation results (on ligand-binding sites) from the COFACTOR, TM-SITE and S-SITE programs.)

  Ligand binding sites


Click
to view		RankC-scoreCluster
size		 PDB
Hit		 Lig
Name		 Download
Complex		 Ligand Binding Site Residues
10.08 3 1jpwC PEPTIDE Rep, Mult 373,374,380,430,433
20.03 1 N/A N/A N/A 119
30.03 1 N/A N/A N/A 534,542,592,593,595
40.03 1 N/A N/A N/A 445
50.03 1 N/A N/A N/A 459,460,493


Download the residue-specific ligand binding probability, which is estimated by SVM.
Download the all possible binding ligands and detailed prediction summary.
Download the templates clustering results.
(a)C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)Cluster size is the total number of templates in a cluster.
(c)Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column.
Mult is the complex structures with all potential binding ligands in the cluster.

  Enzyme Commission (EC) numbers and active sites


Click
to view		RankCscoreECPDB
Hit		TM-scoreRMSDaIDENaCovEC NumberActive Site Residues
10.0633h09B0.291 8.580.0360.454 3.4.21.72  154,197,210
20.0632pziA0.274 7.590.0500.403 2.7.11.1  NA
30.0631ej6A0.278 9.220.0560.467 2.7.7.50  NA
40.0633ecqB0.273 8.090.0410.415 3.2.1.97  NA
50.0632uv8G0.282 9.150.0450.463 2.3.1.86  NA

 Click on the radio buttons to visualize predicted active site residues.
(a)CscoreEC is the confidence score for the EC number prediction. CscoreEC values range in between [0-1];
where a higher score indicates a more reliable EC number prediction.
(b)TM-score is a measure of global structural similarity between query and template protein.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided
by length of the query protein.

  Gene Ontology (GO) terms
Top 10 homologous GO templates in PDB 
RankCscoreGOTM-scoreRMSDaIDENaCovPDB HitAssociated GO Terms
1 0.080.3550 8.56 0.05 0.553p8cA GO:0051015 GO:0030032 GO:0003779 GO:0048675 GO:0045202 GO:0031529 GO:0030027 GO:0005515 GO:0042995 GO:0005845 GO:0001726 GO:0005737 GO:0019717 GO:0048471 GO:0048365 GO:0030154 GO:0008360 GO:0007275 GO:0030054 GO:0007399
2 0.070.3483 8.09 0.05 0.523p8cB GO:0005515 GO:0007417 GO:0016021 GO:0042995 GO:0016020 GO:0031258 GO:0006915 GO:0005886
3 0.070.3195 8.09 0.05 0.473m62A GO:0005737 GO:0006950 GO:0016567 GO:0030433 GO:0005515 GO:0006511 GO:0034450 GO:0005634 GO:0016874 GO:0000151 GO:0004842
4 0.070.2927 8.04 0.06 0.453k8pD GO:0016020 GO:0005789 GO:0005783 GO:0016192 GO:0070939 GO:0005515 GO:0005635 GO:0006810 GO:0032581 GO:0015031 GO:0005777 GO:0006890 GO:0003674
5 0.060.2908 8.58 0.04 0.453h09B GO:0004252 GO:0006508
6 0.060.2983 7.20 0.05 0.422rfoA GO:0005643 GO:0006810
7 0.060.2950 7.45 0.04 0.433icqT GO:0003723 GO:0006810 GO:0006605 GO:0005525 GO:0005737 GO:0005643 GO:0000049 GO:0005829 GO:0005635 GO:0008033 GO:0005634 GO:0005488
8 0.060.2838 7.63 0.03 0.413a6pA GO:0005515 GO:0015031 GO:0005730 GO:0005829 GO:0003723 GO:0000049 GO:0031047 GO:0005634 GO:0006810 GO:0006886 GO:0005654 GO:0008565 GO:0005488 GO:0005737 GO:0006611
9 0.060.2845 7.78 0.05 0.423opbA GO:0005515 GO:0017022 GO:0008298 GO:0030036 GO:0005737 GO:0007533 GO:0005488
10 0.060.2837 7.20 0.04 0.402z6gA GO:0005488 GO:0005515 GO:2000008 GO:0016020 GO:0046332 GO:0048471 GO:0005886 GO:0034747 GO:0043065 GO:0071363 GO:0005915 GO:0070412 GO:0008022 GO:0045893 GO:0005938 GO:0005912 GO:0045768 GO:0005813 GO:0032993 GO:0061154 GO:0071681 GO:0005924 GO:0005102 GO:0035257 GO:0090279 GO:0014704 GO:0008285 GO:0010909 GO:0048660 GO:0060070 GO:0016342 GO:0005902 GO:0034333 GO:0044325 GO:0044336 GO:0044334 GO:0045294 GO:0070369 GO:0030057 GO:0045296 GO:0016337 GO:0042493 GO:0048262 GO:0000578 GO:0005634 GO:0005913 GO:0030054 GO:0070602 GO:0019900 GO:0005667 GO:0019899 GO:0007155 GO:0019903 GO:0034394 GO:0008134 GO:0030331 GO:0003713 GO:0030997 GO:0016055 GO:0005911 GO:0034742 GO:0070411 GO:0005737 GO:0016328 GO:0032355


Consensus prediction of GO terms		 
Molecular Function GO:0048365 GO:0051015 GO:0034450 GO:0004252
GO-Score 0.08 0.08 0.07 0.06
Biological Process GO:0048675 GO:0030032 GO:0008360 GO:0031529 GO:0007417 GO:0006915 GO:0016567 GO:0030433 GO:0006950 GO:0006890
GO-Score 0.08 0.08 0.08 0.08 0.07 0.07 0.07 0.07 0.07 0.07
Cellular Component GO:0001726 GO:0030054 GO:0005845 GO:0019717 GO:0048471 GO:0045202 GO:0016021 GO:0031258 GO:0000151 GO:0005789
GO-Score 0.08 0.08 0.08 0.08 0.08 0.08 0.07 0.07 0.07 0.07

(a)CscoreGO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)TM-score is a measure of global structural similarity between query and template protein.
(c)RMSDa is the RMSD between residues that are structurally aligned by TM-align.
(d)IDENa is the percentage sequence identity in the structurally aligned region.
(e)Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on CscoreGO of the template.


[Click on S802700_results.tar.bz2 to download the tarball file including all modeling results listed on this page]


Please cite the following articles when you use the I-TASSER server:
  • Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. Cell Reports Methods, 1: 100014 (2021).
  • Chengxin Zhang, Peter L. Freddolino, and Yang Zhang. COFACTOR: improved protein function prediction by combining structure, sequence and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017).
  • Jianyi Yang, Yang Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.