Structure of PDB 5x6c Chain A

Receptor sequence

>5x6cA (length=539) Species: 243232 (Methanocaldococcus jannaschii DSM 2661)

MRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVM
ETIERLRQAYLRMGFEEMINPVIVDEMEIYKQFGPEAMAVLDRCFYLAGL
PRPDVGLGNEKVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFE
IAKALNVSNEMGLKVLETAFPEFKDLKPESTTLTLRSHMTSGWFITLSSL
IKKRKLPLKLFSIDRCFRREQREDRSHLMSYHSASCVVVGEDVSVDDGKV
VAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPKLGEWIEVATF
GVYSPIALAKYNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRL
SDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESPCSVEVKREFN
FNGERRVIKVEIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQ
YIPILKKAKEEGVSTNIRYIDGIIYKLVAKIEEALVSNVDEFKFRVPIVR
SLSDINLKIDELALKQIMGENKVIDVRGPVFLNAKVEIK

3D structure

• PDB: 5x6c Structural basis for tRNA-dependent cysteine biosynthesis
• Chain: A
• Resolution: 3.101 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.27: O-phosphoserine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	D43 D372 K373 V374 I376 V496 S497 K518	D33 D362 K363 V364 I366 V486 S487 K508
BS02	peptide	A	I376 D378 E379 F411 N412 E414 R416 V496 N498 D500	I366 D368 E369 F401 N402 E404 R406 V486 N488 D490
BS03	ATP	A	R228 E230 H237 L238 Y241 K281 E306 G333 R336	R218 E220 H227 L228 Y231 K271 E296 G323 R326

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004826 phenylalanine-tRNA ligase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0043816 phosphoserine-tRNA(Cys) ligase activity

Biological Process

• GO:0006412 translation
• GO:0006432 phenylalanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5x6c, PDBe:5x6c, PDBj:5x6c
• PDBsum: 5x6c
• PubMed: 29142195
• UniProt: Q59054|SEPS_METJA O-phosphoserine--tRNA(Cys) ligase (Gene Name=sepS)