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Receptor Information

>8b9b Chain 2 (length=568) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]

LREELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSV
YGARIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAM
EATELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTR
RTGVFPQLKYVKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDV
SKPGEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRSWTEEEERE
FRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSI
RGDINVLLLGDPGTAKSQILKYVEKTAHRAVFAALVLADKGVCLIDEFDQ
DRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNSTLPLAQ
NVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPELQRQRK
KEEEISPIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYADLRRESIST
GSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVVVDSFVDAQK
VSVRRQLRRSFAIYTLGH

Ligand ID

ANP

InChI

InChI=1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1

InChIKey

PVKSNHVPLWYQGJ-KQYNXXCUSA-N

SMILES

Software	SMILES
OpenEye OEToolkits 1.7.0	c1nc(c2c(n1)n(cn2)C3C(C(C(O3)COP(=O)(O)OP(=O)(NP(=O)(O)O)O)O)O)N
CACTVS 3.370	Nc1ncnc2n(cnc12)[CH]3O[CH](CO[P](O)(=O)O[P](O)(=O)N[P](O)(O)=O)[CH](O)[CH]3O
CACTVS 3.370	Nc1ncnc2n(cnc12)[C@@H]3O[C@H](CO[P](O)(=O)O[P](O)(=O)N[P](O)(O)=O)[C@@H](O)[C@H]3O
ACDLabs 12.01	O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O
OpenEye OEToolkits 1.7.0	c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O)(O)O[P@@](=O)(NP(=O)(O)O)O)O)O)N

Formula

C10 H17 N6 O12 P3

Name

PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER

PDB chain

8b9b Chain 2 Residue 1500 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]

Receptor-Ligand Complex Structure

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Structure summary

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PDB	8b9b How Pol alpha-primase is targeted to replisomes to prime eukaryotic DNA replication.
Resolution	3.5 Å
Binding residue (original residue number in PDB)	G546 A548 K549 S550 Q551 L695
Binding residue (residue number reindexed from 1)	G313 A315 K316 S317 Q318 L430
Annotation score	3

Enzyme Commision number

3.6.4.12: DNA helicase.

	Molecular Function
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003688	DNA replication origin binding
GO:0003697	single-stranded DNA binding
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008270	zinc ion binding
GO:0016787	hydrolase activity
GO:0016887	ATP hydrolysis activity
GO:0017116	single-stranded DNA helicase activity
GO:0043138	3'-5' DNA helicase activity
GO:0046872	metal ion binding

	Biological Process
GO:0000727	double-strand break repair via break-induced replication
GO:0006260	DNA replication
GO:0006267	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268	DNA unwinding involved in DNA replication
GO:0006270	DNA replication initiation
GO:0006271	DNA strand elongation involved in DNA replication
GO:0006279	premeiotic DNA replication
GO:0006974	DNA damage response
GO:0032508	DNA duplex unwinding
GO:1902975	mitotic DNA replication initiation
GO:1905775	negative regulation of DNA helicase activity

	Cellular Component
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005656	nuclear pre-replicative complex
GO:0005737	cytoplasm
GO:0031261	DNA replication preinitiation complex
GO:0031298	replication fork protection complex
GO:0042555	MCM complex
GO:0043596	nuclear replication fork
GO:0071162	CMG complex

PDB	RCSB:8b9b, PDBe:8b9b, PDBj:8b9b
PDBsum	8b9b
PubMed	37506699
UniProt	P29469\|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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Structure of PDB 8b9b Chain 2 Binding Site BS01