Zhanglab forum

I submitted my job on the I-TASSER server (ID ) and get the results. According to the server help page, a higher c-score (i.e., more positive) is better. Is it true? accordingly between 5 top predicted models for my structure (with C-score -2.72, -2.32, -1.20, -3.39, and -2.94) I choose the model 3 with c-score -1.20. However, TM-score and RMSD are only provided for model 1 (C-score -2.72). In this regard, I validate the structures of model1 and model3 by saves v6.0 and proSA servers and get the better results for model 3.
How could we obtain these indexes for other models? Moreover, I compared the 3d of model3 with 10-top ranked model presented in I-TASSER results page with Swiss model structural assessment tool. Is this work to calculate TM-score and RMSD OK?
I will be very thankful if you guide me as rapid as possible.
Sincerely yours

Yes, your understanding of the C-score is correct. The C-score provided by the I-TASSER server reflects the confidence in the predicted model, and a higher (more positive) C-score generally indicates a better model. So, choosing model 3 with a C-scoregeometry dash of -1.20 over model 1 with a lower C-score (-2.72) is a reasonable decision, especially since you've obtained better validation results for model 3 on SAVES v6.0 and ProSA.