Contacts and Distance Restrictions Option

[mpetgrav]

Hi,
I was wondering, for ITASSER residue/atom distance restrictions and contacts option (when submitting your sequence), why is it that not all of my generated models had these restrictions in place? For instance, I asked for a disulfide bond between two SG atoms, and contacts between these two residues and only one model had this disulfide bond

Thank you!

[xiaogenz]

I think it is possible that the I-TASSER restraints has a stronger weight than your provided restraints. So the simulation forced the model folding according to the I-TASSER force filed.