Zhanglab forum

One possible reason is that the weight of your provided distance constraint is less than that of the I-TASSER potential, since you just provided one residue pair. Thanks for your quick reply xiaogenz, I have just posted the first restraint (/line) to show the formatting, but I submitted 23 ...

Thanks for your quick reply xiaogenz, I have just posted the first restraint (/line) to show the formatting, but I submitted 23 restraints all with the same distance and all between alpha carbons like this: DIST 216 CA 304 CA 9.8 DIST 79 CA 154 CA 9.8 DIST 118 CA 196 CA 9.8 DIST 59 CA 240 CA 9.8 ...

I have been trying to use the tool to predict the structure of a protein with distance restraints from in-vitro crosslinking data. I have tried to follow the instructions in https://zhanglab.dcmb.med.umich.edu/I-TASSER/restraint.html for preparing the restraints .txt file and submitted several ...