Target: visualization of the input sequence with the predicted domain segments shown in different colors;
Domain Conservation Score: deduced from multiple threading alignments.
It allows the user to move the horizontal line to change the cutoff;
or to move the vertical line to refine the boundary; or add new boundary by right-click;
Predicted Secondary Structure: the predicted secondary structure by
PSSpred which can be used as reference to refine the boundaries;
Predcited Solvent Accessibilty: the predicted solvent accessibility which can be
used together with predicted secondary structure to refine the boundaries;
Predicted domain: the domain segment predicted by the Domain Conversation Score with the default cutoff;
Optimization and DCD detection: boundaries optimization or discontinuous protein domains detection
by the boundary cluster methods based on the predicted boundaries;
Boundaries by user: change or refinement of the predicted boundaries by the users. The change can be saved,
rollbacked or initiated. Furthermore, it also allows the user to re-detect the discontinuous domains according
to user’s editing by clicking the DCD Dectect button.
DCD Detection by user: the server will re-detect the discontinuous domains if there are not less than 3 segments after the user's edit. The DCD detection by the boundary clustering method is very fast. If the boundary clustering method does not detect discontinuous domains, DomEX will be used to further detect. It will take about 2-5 hours to detect the putative discontinuous domains with DomEx, which need search a library with 5,308,138 single domain sequences for each putative domain by Psi-blast.