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COFACTOR result for job id 76548

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>76548 (915 residues)
MSNKYKDTLLIGQTDFDMRANLNQKEPKFEQFWQENQIYNKKLKLNENKKVFVLHDGPPY
ANGDLHIGHALNKTLKDFIIRFKNATGYYAPFIMGWDTHGLPIESAVTKMGVDRKSMSSV
AFRELCYKYALEQVSNQANQFNRLGMFTDYDTKYVTLTHDFEMSELRLFEKMYQKGLIYK
DLKPIYWSPSSESALADSEIIYKDITSPSIYVGCDVVNSDEFKNTQLIIWTTTPWTLPSN
QLIAVGSKIKYSLIQVENLDKQFILASDLINQVSQNIGWENVKVIKEIDANKLVGLNYAH
PLYDTKISKVVLGHHVTSESGSGLVHIASGFGEDDFLIAKQNNIKPFAPIDNQGKFTTEI
SDLDPQLVGMFYDDTNKIITKKLEENKKLLKLKFLTHSYPHDWRTKKPVIYRCTLQWFVN
LAPVKNEILKNVDQINTHPKWAKKRLYQVLDERTDWTISRQRLWGVPIIAFYDQNNNLVL
NEQILNYAINKIEQVGTNAWFSLDADEFLPDEYKNKSLKKEKDILDVWFDSGSSAIALSQ
KYPNLKLPYDMYLEGNDQYRGWFNASMINSTIYSNTSPYKQLVSHGMTTDEKGNKMSKSL
GNGVDPIAFANDLGADILRLWVASTDFTDDQKIGKEIIKQISESYRKIRNTMRFILANLN
DFNAKTDYQTKLSEVDMYSLFNLTSFKNKVIQAYEELNFSLVYTLVMNYVTKNLSAFYLD
FIKDILYINSKNDLRRRQVQTVLYEQLYCLIDVLRPILVHTIEEVYQNLNDNNKVESVHL
LDNKEQNFVYDKEFISKWDQVMILRDDVNKALEISRENKIINKGFEAVVNIKLDKEYDDL
KNIKDLSQIFIVNSINFVDNIDNSFIKTNISSIKVEQKQGLKCQRCWQIFDNLIDDEICN
HCNHVVKSLLETKCE

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1ffyA	0.98	0.41	0.464	0.983	Download
2	1ivsA	0.73	4.57	0.196	0.838	Download
3	1jzsA	0.73	5.06	0.242	0.847	Download
4	1wz2A	0.64	4.93	0.163	0.739	Download
5	6q89A	0.54	5.20	0.162	0.633	Download
6	4aq7A	0.54	5.05	0.144	0.627	Download
7	2v0gA	0.54	5.05	0.164	0.626	Download
8	5ah5A	0.53	4.96	0.163	0.612	Download
9	3ziuB	0.51	3.45	0.214	0.554	Download
10	5gl7A	0.51	3.75	0.120	0.558	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0004812	1.00	aminoacyl-tRNA ligase activity
GO:0004822	0.69	isoleucine-tRNA ligase activity
GO:0016787	0.56	hydrolase activity
GO:1901363	0.52	heterocyclic compound binding
GO:0097159	0.52	organic cyclic compound binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

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[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0090304	1.00	nucleic acid metabolic process
GO:0044699	1.00	single-organism process
GO:0006418	0.99	tRNA aminoacylation for protein translation
GO:0006428	0.68	isoleucyl-tRNA aminoacylation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044444	1.00	cytoplasmic part
GO:0005737	1.00	cytoplasm
GO:0005829	0.99	cytosol

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.300	1ffyA	0.981	0.41	0.464	0.983	6.1.1.5	NA
2	0.126	1gaxA	0.733	4.59	0.196	0.838	6.1.1.9	NA
3	0.071	1a8hA	0.470	3.15	0.196	0.506	6.1.1.10	NA
4	0.060	1irxB	0.293	4.73	0.065	0.342	6.1.1.6	NA
5	0.060	1j09A	0.276	3.83	0.062	0.306	6.1.1.17	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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