[back]

COFACTOR result for job id 76533

[Download result.tar.bz2 for all prediction results]

User Input

>76533 (226 residues)
MNLIDQIKNTLIVSCQAVDDEPLNDSYVLSKMCYALVLGGAKVLRLSQVEHIKKIKEVVN
VPIIGLIKKHYDNSEVFITPTIKEVDQLVDLKVDIIALDATLRKRPDQDLTNLIKTIKTK
YPNQLLMADCSNINDAINAQNLGFDLISTTLRGYTKDTLNHNNIENDYQFLKDLKKVITK
PIIAEGGIWTPQQAKEILNLGIHSVVVGSAITRLHLIVKYWNDNLK

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5zknA	0.97	0.52	0.518	0.982	Download
2	1yxyA	0.96	0.92	0.422	0.987	Download
3	4utuA	0.95	1.10	0.457	0.978	Download
4	5zjbA	0.91	1.52	0.320	0.969	Download
5	1y0eA	0.91	1.56	0.465	0.951	Download
6	3q58A	0.89	1.59	0.353	0.951	Download
7	3igsB	0.89	1.72	0.391	0.951	Download
8	1ox4B	0.71	3.45	0.128	0.876	Download
9	1hg3A	0.70	3.08	0.120	0.841	Download
10	4xbkA	0.70	3.29	0.126	0.836	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	1.00	catalytic activity
GO:0016853	0.89	isomerase activity
GO:0016857	0.87	racemase and epimerase activity, acting on carbohydrates and derivatives
GO:0047465	0.83	N-acylglucosamine-6-phosphate 2-epimerase activity
GO:0016829	0.55	lyase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0008152	0.99	metabolic process
GO:0071704	0.98	organic substance metabolic process
GO:0044237	0.96	cellular metabolic process
GO:0044281	0.95	small molecule metabolic process
GO:0044763	0.94	single-organism cellular process
GO:0006082	0.91	organic acid metabolic process
GO:1901135	0.90	carbohydrate derivative metabolic process
GO:0006040	0.89	amino sugar metabolic process
GO:1901575	0.88	organic substance catabolic process
GO:0019752	0.87	carboxylic acid metabolic process
GO:0044282	0.85	small molecule catabolic process
GO:0046395	0.84	carboxylic acid catabolic process
GO:0006054	0.84	N-acetylneuraminate metabolic process
GO:0019262	0.83	N-acetylneuraminate catabolic process
GO:0006051	0.83	N-acetylmannosamine metabolic process
GO:0006807	0.74	nitrogen compound metabolic process
GO:1901564	0.72	organonitrogen compound metabolic process
GO:0044249	0.72	cellular biosynthetic process
GO:1901576	0.70	organic substance biosynthetic process
GO:1901566	0.67	organonitrogen compound biosynthetic process
GO:0034641	0.67	cellular nitrogen compound metabolic process
GO:1901360	0.66	organic cyclic compound metabolic process
GO:0046483	0.66	heterocycle metabolic process
GO:0044711	0.66	single-organism biosynthetic process
GO:0006725	0.66	cellular aromatic compound metabolic process
GO:0044271	0.63	cellular nitrogen compound biosynthetic process
GO:0044238	0.63	primary metabolic process
GO:1901362	0.62	organic cyclic compound biosynthetic process
GO:0018130	0.62	heterocycle biosynthetic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.92	cytoplasmic part
GO:0005737	0.81	cytoplasm
GO:0005829	0.79	cytosol
GO:0043229	0.59	intracellular organelle
GO:0043227	0.59	membrane-bounded organelle
GO:0043231	0.58	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.500	3igsB	0.886	1.72	0.391	0.951	5.1.3.9	12,16,78,131,150,153,183,185,194,207,209,211,213
2	0.322	1xi3A	0.669	2.57	0.191	0.765	2.5.1.3	45
3	0.254	2czfA	0.515	3.81	0.156	0.677	4.1.1.23	98
4	0.193	1vlwB	0.687	2.40	0.160	0.783	4.1.3.16	NA
5	0.169	3ng3A	0.695	3.30	0.104	0.832	4.1.2.4	149

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.32	1y0eB	0.942	1.57	0.459	0.982	1.32	PO4	Download	186,187,207,208,209
2	0.14	1jcmP	0.721	2.79	0.130	0.841	0.72	137	Download	14,16,154,185,186,187,206,207,208,209
3	0.14	3igsA	0.905	1.65	0.397	0.969	0.67	16G	Download	68,77,154,209
4	0.03	1piiA	0.726	3.01	0.135	0.858	0.71	PO4	Download	186,189,209

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

[back]