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COFACTOR result for job id 76472

[Download result.tar.bz2 for all prediction results]

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>76472 (317 residues)
MENKIKIVFCGTPKIGADVLKALIEMNQVEVVLVISQPDKPIGRKKQIVYTPVKKLALEN
NLKVVQPNKIGEIYDDLAKLEFDFLITCAFGQFIPTKILKLAKIDSINFHGSLLPKLRGG
APIQYAIKNGDKKTGITIMQMVKQMDAGDYYVQESIDILDSDDSGSLFEKMGQLAYSMCK
KYLVDIYNHKFELIKQNEDEVTFCKNISSEEEKINWNNTSLDIFNLIRSLSPSPISYTTI
NNQRYKIKSSKVIDLDNQNKNVMPGTIIDINKQGIVVKTLDKALLILEIQKEGKKMILAS
NYYLNKLSDLKINDKFD

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5uaiA	0.96	0.59	0.286	0.972	Download
2	2fmtA	0.95	1.30	0.356	0.984	Download
3	3q0iA	0.90	1.19	0.332	0.931	Download
4	3rfoA	0.90	2.03	0.423	0.962	Download
5	3tqqA	0.88	1.57	0.308	0.931	Download
6	1z7eD	0.87	1.85	0.259	0.927	Download
7	2cfiA	0.82	2.62	0.257	0.921	Download
8	5uijA	0.76	2.83	0.188	0.861	Download
9	4xczA	0.76	2.98	0.170	0.861	Download
10	4lxqA	0.72	2.95	0.226	0.830	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	1.00	catalytic activity
GO:0016740	0.96	transferase activity
GO:0016742	0.95	hydroxymethyl-, formyl- and related transferase activity
GO:0004479	0.94	methionyl-tRNA formyltransferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

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[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	1.00	cellular process
GO:0071704	0.99	organic substance metabolic process
GO:0044237	0.99	cellular metabolic process
GO:0034641	0.98	cellular nitrogen compound metabolic process
GO:1901360	0.97	organic cyclic compound metabolic process
GO:0046483	0.97	heterocycle metabolic process
GO:0044238	0.97	primary metabolic process
GO:0006725	0.97	cellular aromatic compound metabolic process
GO:0043170	0.95	macromolecule metabolic process
GO:0006139	0.95	nucleobase-containing compound metabolic process
GO:0090304	0.93	nucleic acid metabolic process
GO:0044260	0.93	cellular macromolecule metabolic process
GO:0034660	0.92	ncRNA metabolic process
GO:0006399	0.91	tRNA metabolic process
GO:0019538	0.90	protein metabolic process
GO:0044267	0.89	cellular protein metabolic process
GO:0043412	0.89	macromolecule modification
GO:0006400	0.88	tRNA modification
GO:0019988	0.87	charged-tRNA amino acid modification
GO:0071951	0.86	conversion of methionyl-tRNA to N-formyl-methionyl-tRNA
GO:0006413	0.86	translational initiation
GO:1901564	0.81	organonitrogen compound metabolic process
GO:0044699	0.72	single-organism process
GO:0044763	0.70	single-organism cellular process
GO:0044281	0.70	small molecule metabolic process
GO:0006082	0.59	organic acid metabolic process
GO:0044249	0.53	cellular biosynthetic process
GO:0019752	0.53	carboxylic acid metabolic process
GO:1901576	0.51	organic substance biosynthetic process
GO:0006520	0.51	cellular amino acid metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005829	1.00	cytosol
GO:0043227	0.95	membrane-bounded organelle
GO:0043231	0.93	intracellular membrane-bounded organelle
GO:0005739	0.91	mitochondrion

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.430	1meoA	0.506	2.45	0.166	0.561	2.1.2.2	108,110
2	0.195	2cfiA	0.822	2.62	0.257	0.921	1.5.1.6	NA
3	0.081	1c2tA	0.532	2.01	0.158	0.571	2.1.2.2	NA
4	0.069	3da8A	0.521	2.59	0.185	0.584	2.1.2.2	NA
5	0.066	1cddA	0.455	2.09	0.117	0.495	2.1.2.2	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.61	2fmtB	0.952	1.31	0.356	0.984	1.43	FME	Download	15,89,90,108,110,111,119,120
2	0.17	1rbzA	0.523	2.11	0.191	0.565	1.08	KT5	Download	14,17,38,87,89,91,92,93,94,108,110,111,119,120,141,142,143,145,146,172,175
3	0.17	1c2tA	0.532	2.01	0.158	0.571	1.16	GAR	Download	17,18,89,90,91,109,111,175
4	0.13	1jkxB	0.529	1.94	0.169	0.568	0.98	138	Download	14,17,18,19,38,89,90,91,92,93,94,99,109,110,111,141,175
5	0.04	3nrbC	0.504	2.89	0.158	0.580	0.89	IMD	Download	12,36,37,91,92

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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