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COFACTOR result for job id 76428

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>76428 (358 residues)
MTKHEIINELLEKNNADAILLYSPENRYWFSKFHSSLGYLIITKTQSHLFLDGRYITAAR
NNKNINKDIELHHFSKNLKQDLIDILNQNNVKTLAFESDWTYFEQYQAYKNHWFKDFDLI
GINCSKIRMIKDDWEIANIKKACDITDQVFQAALDFIKPGITEKQLQRFIDDKFLEFGAD
KISFDTIIASGVNGSMPHAVPSDKVINNNELITIDMGCFYNGYCSDQTRTIALGDVDPKL
VEIYNIVYEAQSLGISLVKEGVIAGDIHKQVYDFIDKKGYGKYFDHGLGHGIGVEIHEEP
SVGSTGSEVLKENMTITIEPGIYIPDLGGVRIEDDVLVTKTGCKLLTSSPRILLKLQK

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	5cnxA	0.98	0.77	0.324	0.992	Download
2	3q6dA	0.97	0.99	0.382	0.986	Download
3	4fkcA	0.93	1.78	0.306	0.986	Download
4	1wy2A	0.93	1.41	0.377	0.964	Download
5	4r60A	0.92	2.03	0.247	0.989	Download
6	1wn1A	0.92	1.71	0.342	0.972	Download
7	4egeA	0.91	2.27	0.237	0.989	Download
8	1chmA	0.91	2.24	0.187	0.986	Download
9	1kp0A	0.91	2.22	0.184	0.986	Download
10	2zsgA	0.90	2.22	0.326	0.978	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	1.00	catalytic activity
GO:0016787	0.99	hydrolase activity
GO:0008233	0.97	peptidase activity
GO:0043167	0.96	ion binding
GO:0046872	0.95	metal ion binding
GO:0008238	0.95	exopeptidase activity
GO:0008237	0.87	metallopeptidase activity
GO:0004177	0.85	aminopeptidase activity
GO:0016805	0.76	dipeptidase activity
GO:0102009	0.75	proline dipeptidase activity

Download full result of the above consensus prediction.

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(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0071704	0.99	organic substance metabolic process
GO:0044238	0.99	primary metabolic process
GO:0043170	0.98	macromolecule metabolic process
GO:0019538	0.97	protein metabolic process
GO:0006508	0.94	proteolysis
GO:0009987	0.90	cellular process
GO:0044237	0.82	cellular metabolic process
GO:0006807	0.69	nitrogen compound metabolic process
GO:0034641	0.61	cellular nitrogen compound metabolic process
GO:1901564	0.53	organonitrogen compound metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.90	cytoplasmic part
GO:0043226	0.84	organelle
GO:0043227	0.82	membrane-bounded organelle
GO:0005737	0.71	cytoplasm
GO:0005829	0.65	cytosol
GO:0043229	0.63	intracellular organelle
GO:0043231	0.60	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.553	1wn1A	0.922	1.71	0.342	0.972	3.4.-.-	129,132,223,314
2	0.542	2iw2A	0.870	2.83	0.234	0.980	3.4.13.9	297,319
3	0.412	2dfiA	0.540	2.06	0.234	0.581	3.4.11.18	301,319
4	0.232	2zsgA	0.901	2.22	0.326	0.978	3.4.11.9	297,319
5	0.220	1pv9A	0.898	1.63	0.384	0.939	3.4.13.9	297,319

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.74	1wy2A	0.930	1.41	0.377	0.964	1.40	ZN	Download	290,319,333
2	0.72	3q6dA	0.967	0.99	0.382	0.986	0.94	CA	Download	215,226,228,333
3	0.46	1n51A	0.901	2.22	0.266	0.975	1.11	III	Download	184,198,215,226,286,290,296,297,319,331,333
4	0.46	2v3yA	0.898	2.25	0.264	0.975	1.08	III	Download	198,215,286,287,290,306,319,331
5	0.25	2gz5A	0.613	2.67	0.203	0.670	0.81	OVA	Download	184,198,286,287,289,296,319,321
6	0.08	1pv90	0.898	1.63	0.384	0.939	1.24	III	Download	54,96,98,100,164,165,168,171,172,175,176,180,181,185,196,197,198,285,305

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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