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COFACTOR result for job id 76410

[Download result.tar.bz2 for all prediction results]

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>76410 (205 residues)
MSKKYYAIKKGLKPGIYTTWDEAKKQVENYSNAVYKSFSTLKEAEDFLNDSNKQSDNLNS
DKNSCIAYTDGSYNTLDNTFSYGVVVFWKNREFHLSQRFDNQNISSLRNVAGEVLAVKQT
IMFCVANKIKKVLICHDYQGVSKWALDQWKANLDFTKEYKEFFNKYKNQVEVEFKWIKSH
TNNKYNDLADKLAKNASLEFVLKEV

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1goaA	0.59	2.42	0.190	0.668	Download
2	2e4lA	0.59	2.40	0.191	0.663	Download
3	2qkkA	0.59	2.29	0.152	0.663	Download
4	2ehgA	0.58	2.48	0.191	0.663	Download
5	4ibnA	0.58	2.61	0.161	0.668	Download
6	3h08B	0.58	2.02	0.177	0.634	Download
7	1rilA	0.57	2.47	0.157	0.649	Download
8	3hstB	0.56	2.37	0.149	0.634	Download
9	4e19A	0.56	2.27	0.147	0.629	Download
10	2lsnA	0.55	3.27	0.203	0.673	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0016787	1.00	hydrolase activity
GO:0004523	0.99	RNA-DNA hybrid ribonuclease activity
GO:0003676	0.84	nucleic acid binding

Download full result of the above consensus prediction.

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(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

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[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

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[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044238	1.00	primary metabolic process
GO:0043170	1.00	macromolecule metabolic process
GO:0034641	1.00	cellular nitrogen compound metabolic process
GO:0006259	0.99	DNA metabolic process
GO:0016070	0.98	RNA metabolic process
GO:0006401	0.97	RNA catabolic process
GO:0043137	0.96	DNA replication, removal of RNA primer

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005737	1.00	cytoplasm
GO:0044444	0.83	cytoplasmic part
GO:0043229	0.82	intracellular organelle
GO:0043231	0.81	intracellular membrane-bounded organelle
GO:0005634	0.79	nucleus
GO:0005739	0.65	mitochondrion
GO:0044446	0.63	intracellular organelle part
GO:0044427	0.61	chromosomal part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.396	3hyfA	0.537	2.67	0.164	0.620	3.1.26.4	180
2	0.221	3h08B	0.576	2.02	0.177	0.634	3.1.26.4	109,113,137
3	0.081	1jl2A	0.588	2.29	0.193	0.658	3.1.26.4	180
4	0.078	1mu2A	0.528	2.85	0.144	0.624	3.4.23.47	180
5	0.077	2qkkA	0.587	2.29	0.152	0.663	3.1.26.4	180

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.19	1wsfD	0.590	2.56	0.197	0.668	1.61	MN	Download	70,71,113,137
2	0.11	3lp1A	0.535	2.82	0.137	0.629	1.25	LP8	Download	70,113,137,179,180,190
3	0.07	2qkkI	0.586	2.04	0.158	0.649	0.99	RQA	Download	70,71,72,73,74,75,76,109,113,137,138,139,175,178,179,194
4	0.03	2qkbB	0.587	2.18	0.148	0.658	0.84	RQA	Download	70,71,72,73,74,76,113,138,140,176

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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