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COFACTOR result for job id 76374

[Download result.tar.bz2 for all prediction results]

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>76374 (185 residues)
MHIISGKYKKMKLQTLDSSITRPTLTRIKEDMFNIISNYFIFENKTSLDLFGGSGSLSIE
GLSRGIKFAIINDLNKDANKIISFNLKKIPTSDYVLYQKDYLELLNLLKIQHQKVDLVYL
DPPFKEIDYYYVVFDFLINNNLLNDWAIIISETNQKLDLTKIKDLNLLKFKDYNKKYLYI
FRLEK

Download query sequence and structure

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3p9nA	0.94	0.90	0.213	0.962	Download
2	2fhpA	0.92	0.97	0.320	0.946	Download
3	2fpoC	0.85	1.98	0.257	0.946	Download
4	1ws6A	0.85	1.50	0.185	0.908	Download
5	2iftA	0.84	1.74	0.276	0.919	Download
6	3axsA	0.81	2.29	0.145	0.930	Download
7	3vseA	0.80	2.59	0.159	0.946	Download
8	4oa8A	0.80	2.31	0.099	0.919	Download
9	3c3yA	0.80	2.71	0.109	0.935	Download
10	3cbgA	0.80	2.44	0.129	0.913	Download

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0008757	1.00	S-adenosylmethionine-dependent methyltransferase activity
GO:0008170	1.00	N-methyltransferase activity
GO:1901363	0.89	heterocyclic compound binding
GO:0097159	0.89	organic cyclic compound binding
GO:0003676	0.88	nucleic acid binding
GO:0008173	0.75	RNA methyltransferase activity
GO:0008649	0.74	rRNA methyltransferase activity
GO:0052913	0.73	16S rRNA (guanine(966)-N(2))-methyltransferase activity
GO:0008276	0.65	protein methyltransferase activity
GO:0042054	0.54	histone methyltransferase activity
GO:0035242	0.54	protein-arginine omega-N asymmetric methyltransferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

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[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.97	cellular process
GO:0071704	0.95	organic substance metabolic process
GO:0044238	0.95	primary metabolic process
GO:0044237	0.95	cellular metabolic process
GO:0043170	0.93	macromolecule metabolic process
GO:0044260	0.90	cellular macromolecule metabolic process
GO:0043412	0.89	macromolecule modification
GO:0043414	0.87	macromolecule methylation
GO:0006807	0.82	nitrogen compound metabolic process
GO:0034641	0.80	cellular nitrogen compound metabolic process
GO:1901360	0.78	organic cyclic compound metabolic process
GO:0046483	0.78	heterocycle metabolic process
GO:0006725	0.78	cellular aromatic compound metabolic process
GO:0006139	0.77	nucleobase-containing compound metabolic process
GO:0090304	0.74	nucleic acid metabolic process
GO:0016070	0.70	RNA metabolic process
GO:0034660	0.67	ncRNA metabolic process
GO:0006396	0.66	RNA processing
GO:0065007	0.65	biological regulation
GO:0050789	0.65	regulation of biological process
GO:0034470	0.65	ncRNA processing
GO:0050794	0.64	regulation of cellular process
GO:0009451	0.64	RNA modification
GO:0080090	0.63	regulation of primary metabolic process
GO:0031323	0.63	regulation of cellular metabolic process
GO:0006364	0.62	rRNA processing
GO:0001510	0.61	RNA methylation
GO:0000154	0.61	rRNA modification
GO:0031167	0.60	rRNA methylation
GO:0070475	0.59	rRNA base methylation
GO:0060255	0.57	regulation of macromolecule metabolic process
GO:0051171	0.57	regulation of nitrogen compound metabolic process
GO:2000112	0.56	regulation of cellular macromolecule biosynthetic process
GO:0019219	0.56	regulation of nucleobase-containing compound metabolic process
GO:0010468	0.56	regulation of gene expression
GO:0006355	0.55	regulation of transcription, DNA-templated
GO:0044267	0.50	cellular protein metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.94	cytoplasmic part
GO:0005737	0.84	cytoplasm
GO:0043229	0.79	intracellular organelle
GO:0043231	0.78	intracellular membrane-bounded organelle
GO:0005829	0.69	cytosol

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.445	2iftA	0.837	1.74	0.276	0.919	2.1.1.52	54,56,60,62,64,121
2	0.393	3g07A	0.654	2.90	0.136	0.784	2.1.1.-	73
3	0.148	2cl5A	0.796	2.93	0.128	0.946	2.1.1.6	NA
4	0.143	3bwmA	0.795	2.92	0.123	0.946	2.1.1.6	NA
5	0.137	1dusA	0.772	2.67	0.189	0.913	2.1.1.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.72	2cwwA	0.778	2.42	0.162	0.903	0.87	SAH	Download	51,52,53,57,73,74,100,101,120,122

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Download result.tar.bz2 for all prediction results]

Reference:

Chengxin Zhang, Peter L Freddolino, and Yang Zhang. COFACTOR: Improved protein function prediction by combining structure, sequence, and protein-protein interaction information. Nucleic Acids Research, 45: W291-299 (2017)
Ambrish Roy, Jianyi Yang, and Yang Zhang. COFACTOR: An accurate comparative algorithm for structure-based protein function annotation. Nucleic Acids Research, 40:W471-W477 (2012).

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