D-I-TASSER Q9Y6W6

D-I-TASSER results for Q9Y6W6

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q9Y6W6
Protein Name: Dual specificity protein phosphatase 10
Gene Name: DUSP10

Input Sequence in FASTA format

>Q9Y6W6 (482 residues)
MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTY
MPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNN
NENTGSLSPSSGVGSPVSGTPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPF
MEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENT
NEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGA
SAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPL
YHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVI
AYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVET
VV

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q9Y6W6-D1	1-139	139		MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARSLNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSG
2	Q9Y6W6-D2	140-315	176		TPKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKITVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNHENLCDNSLQLQECREVGGGASAASSLLPQPIPTTP
3	Q9Y6W6-D3	316-482	167		DIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGYVINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVIAYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.54

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6f0kB	0.41	6.76	0.025	0.643	model1_6f0kB.pdb.gz
2	6x99A	0.41	7.45	0.048	0.683	model1_6x99A.pdb.gz
3	6sytA	0.40	7.77	0.032	0.685	model1_6sytA.pdb.gz
4	3s4wA	0.40	7.14	0.062	0.647	model1_3s4wA.pdb.gz
5	7ay1A	0.40	7.37	0.038	0.662	model1_7ay1A.pdb.gz
6	3hazA	0.39	7.58	0.066	0.666	model1_3hazA.pdb.gz
7	5vchA	0.39	7.51	0.074	0.662	model1_5vchA.pdb.gz
8	5mz61	0.39	7.22	0.059	0.625	model1_5mz61.pdb.gz
9	2zxqA1	0.39	7.40	0.041	0.647	model1_2zxqA1.pdb.gz
10	1aorA	0.27	7.39	0.046	0.436	model1_1aorA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.88	catalytic activity
GO:0016791	0.85	phosphatase activity
GO:0004721	0.68	phosphoprotein phosphatase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.83	biological regulation
GO:0050789	0.82	regulation of biological process
GO:0050794	0.81	regulation of cellular process
GO:0009987	0.66	cellular process
GO:0044699	0.60	single-organism process
GO:0031323	0.59	regulation of cellular metabolic process
GO:0080090	0.58	regulation of primary metabolic process
GO:0060255	0.57	regulation of macromolecule metabolic process
GO:0042325	0.54	regulation of phosphorylation
GO:0031399	0.54	regulation of protein modification process
GO:0048583	0.53	regulation of response to stimulus
GO:0009966	0.52	regulation of signal transduction
GO:0001932	0.52	regulation of protein phosphorylation
GO:1902531	0.51	regulation of intracellular signal transduction
GO:0043408	0.50	regulation of MAPK cascade

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0043226	0.68	organelle
GO:0043229	0.67	intracellular organelle
GO:0005737	0.67	cytoplasm
GO:0043227	0.65	membrane-bounded organelle
GO:0043231	0.64	intracellular membrane-bounded organelle
GO:0044444	0.54	cytoplasmic part
GO:0005634	0.53	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1ofdA	0.316	7.45	0.030	0.521	1.4.7.1	NA
2	0.060	1r9mB	0.345	7.17	0.038	0.560	3.4.14.5	NA
3	0.060	1yguB	0.373	6.35	0.069	0.552	3.1.3.48	390,392
4	0.060	3cmmC	0.352	7.71	0.066	0.600	6.3.2.19	353,397
5	0.060	1hn0A	0.357	7.54	0.031	0.598	4.2.2.20	350

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1a9xG	0.385	7.06	0.053	0.616	0.24	ADP	complex1.pdb.gz	324,350,397,398,399,400
2	0.01	1ce8A	0.362	7.78	0.036	0.631	0.28	IMP	complex2.pdb.gz	387,413,415,417,418
3	0.01	1c3oA	0.383	7.47	0.036	0.635	0.16	ORN	complex3.pdb.gz	407,412,441
4	0.01	1a9xA	0.378	7.00	0.061	0.602	0.19	PO4	complex4.pdb.gz	331,332,409,439
5	0.01	1ea0B	0.376	7.47	0.043	0.627	0.17	FMN	complex5.pdb.gz	409,410,411,415,416
6	0.01	1jdbB	0.364	7.78	0.039	0.633	0.28	GLN	complex6.pdb.gz	348,393,394
7	0.01	2x2iA	0.383	7.36	0.041	0.631	0.23	QPS	complex7.pdb.gz	323,352,353
8	0.01	1ce8A	0.362	7.78	0.036	0.631	0.23	ADP	complex8.pdb.gz	324,331,332,339
9	0.01	2x2iC	0.374	7.69	0.033	0.649	0.25	QPS	complex9.pdb.gz	332,333,357,358
10	0.01	1a9xE	0.367	7.87	0.032	0.641	0.13	ORN	complex10.pdb.gz	321,331,443

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.