D-I-TASSER Q9Y5A9

D-I-TASSER results for Q9Y5A9

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q9Y5A9
Protein Name: YTH domain-containing family protein 2
Gene Name: YTHDF2

Input Sequence in FASTA format

>Q9Y5A9 (579 residues)
MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYY
SPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQH
GFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAP
GMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWA
DIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPT
QGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSG
FGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSE
DDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYN
TCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQV
LKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q9Y5A9-D1	1-579	579		MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5859

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	7cpxA	0.30	7.76	0.046	0.494	model1_7cpxA.pdb.gz
2	6u5vB	0.30	7.87	0.037	0.496	model1_6u5vB.pdb.gz
3	2pffB	0.30	8.56	0.044	0.525	model1_2pffB.pdb.gz
4	7k66A	0.28	7.27	0.056	0.435	model1_7k66A.pdb.gz
5	7bspA	0.28	7.83	0.045	0.458	model1_7bspA.pdb.gz
6	5xjcA	0.28	7.55	0.029	0.444	model1_5xjcA.pdb.gz
7	5nrlA	0.27	7.81	0.029	0.444	model1_5nrlA.pdb.gz
8	6v85A	0.27	7.75	0.050	0.435	model1_6v85A.pdb.gz
9	4bxsV	0.27	7.94	0.043	0.449	model1_4bxsV.pdb.gz
10	4bujB	0.27	7.58	0.041	0.428	model1_4bujB.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:1901363	0.98	heterocyclic compound binding
GO:0097159	0.98	organic cyclic compound binding
GO:0003723	0.97	RNA binding
GO:1990247	0.78	N6-methyladenosine-containing RNA binding
GO:0044822	0.67	poly(A) RNA binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.89	biological regulation
GO:0050794	0.88	regulation of cellular process
GO:0060255	0.80	regulation of macromolecule metabolic process
GO:0031323	0.80	regulation of cellular metabolic process
GO:0080090	0.79	regulation of primary metabolic process
GO:0010468	0.79	regulation of gene expression
GO:0051171	0.78	regulation of nitrogen compound metabolic process
GO:0048518	0.77	positive regulation of biological process
GO:0048522	0.76	positive regulation of cellular process
GO:2000112	0.74	regulation of cellular macromolecule biosynthetic process
GO:0010608	0.74	posttranscriptional regulation of gene expression
GO:0032268	0.73	regulation of cellular protein metabolic process
GO:0031325	0.73	positive regulation of cellular metabolic process
GO:0010604	0.73	positive regulation of macromolecule metabolic process
GO:0051173	0.72	positive regulation of nitrogen compound metabolic process
GO:0031328	0.72	positive regulation of cellular biosynthetic process
GO:0010628	0.72	positive regulation of gene expression
GO:0010557	0.72	positive regulation of macromolecule biosynthetic process
GO:0006417	0.72	regulation of translation
GO:0032270	0.71	positive regulation of cellular protein metabolic process
GO:0006446	0.71	regulation of translational initiation
GO:0045948	0.70	positive regulation of translational initiation
GO:0009987	0.60	cellular process
GO:0065008	0.57	regulation of biological quality
GO:0043488	0.55	regulation of mRNA stability
GO:1903679	0.53	positive regulation of cap-independent translational initiation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0043226	0.83	organelle
GO:0043229	0.82	intracellular organelle
GO:0043227	0.82	membrane-bounded organelle
GO:0043231	0.81	intracellular membrane-bounded organelle
GO:0005634	0.79	nucleus
GO:0044444	0.69	cytoplasmic part
GO:0032991	0.61	macromolecular complex
GO:0005737	0.61	cytoplasm
GO:0005829	0.60	cytosol
GO:0030529	0.53	intracellular ribonucleoprotein complex
GO:0043232	0.51	intracellular non-membrane-bounded organelle
GO:0000932	0.50	cytoplasmic mRNA processing body

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	9cgtA	0.215	7.87	0.023	0.354	2.4.1.19	117,152,154
2	0.060	3l4uA	0.233	7.56	0.037	0.380	3.2.1.20 3.2.1.3	116,133
3	0.060	1c4aA	0.173	8.50	0.021	0.302	1.12.7.2	NA
4	0.060	1ciuA	0.230	8.11	0.063	0.385	2.4.1.19	NA
5	0.060	1ofdA	0.207	7.12	0.025	0.309	1.4.7.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.01	3jv2A	0.216	7.69	0.056	0.349	0.40	III	complex1.pdb.gz	122,133,134,135

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.