D-I-TASSER Q9NRA1

D-I-TASSER results for Q9NRA1

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q9NRA1
Protein Name: Platelet-derived growth factor C
Gene Name: PDGFC

Input Sequence in FASTA format

>Q9NRA1 (345 residues)
MSLFGLLLLTSALAGQRQGTQAESNLSSKFQFSSNKEQNGVQDPQHERIITVSTNGSIHS
PRFPHTYPRNTVLVWRLVAVEENVWIQLTFDERFGLEDPEDDICKYDFVEVEEPSDGTIL
GRWCGSGTVPGKQISKGNQIRIRFVSDEYFPSEPGFCIHYNIVMPQFTEAVSPSVLPPSA
LPLDLLNNAITAFSTLEDLIRYLEPERWQLDLEDLYRPTWQLLGKAFVFGRKSRVVDLNL
LTEEVRLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPSK
VTKKYHEVLQLRPKTGVRGLHKSLTDVALEHHEECDCVCRGSTGG

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q9NRA1-D1	1-163	163		MSLFGLLLLTSALAGQRQGTQAESNLSSKFQFSSNKEQNGVQDPQHERIITVSTNGSIHSPRFPHTYPRNTVLVWRLVAVEENVWIQLTFDERFGLEDPEDDICKYDFVEVEEPSDGTILGRWCGSGTVPGKQISKGNQIRIRFVSDEYFPSEPGFCIHYNIV
2	Q9NRA1-D2	164-345	182		MPQFTEAVSPSVLPPSALPLDLLNNAITAFSTLEDLIRYLEPERWQLDLEDLYRPTWQLLGKAFVFGRKSRVVDLNLLTEEVRLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPSKVTKKYHEVLQLRPKTGVRGLHKSLTDVALEHHEECDCVCRGSTGG

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.57

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	2pffA	0.41	6.62	0.048	0.696	model1_2pffA.pdb.gz
2	3hmjA	0.41	6.67	0.033	0.699	model1_3hmjA.pdb.gz
3	7m0rE	0.41	4.96	0.162	0.533	model1_7m0rE.pdb.gz
4	5dkxA	0.41	6.75	0.064	0.681	model1_5dkxA.pdb.gz
5	5wbiA	0.40	6.47	0.043	0.652	model1_5wbiA.pdb.gz
6	5m59A	0.40	6.53	0.047	0.678	model1_5m59A.pdb.gz
7	4zhjA	0.40	6.11	0.049	0.632	model1_4zhjA.pdb.gz
8	5f0eA	0.40	6.66	0.078	0.681	model1_5f0eA.pdb.gz
9	5kzwA	0.40	6.62	0.058	0.658	model1_5kzwA.pdb.gz
10	4zm6A	0.40	6.50	0.047	0.658	model1_4zm6A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005102	0.80	receptor binding
GO:0070851	0.72	growth factor receptor binding
GO:0005161	0.69	platelet-derived growth factor receptor binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.96	biological regulation
GO:0050789	0.95	regulation of biological process
GO:0044699	0.94	single-organism process
GO:0050794	0.92	regulation of cellular process
GO:0048583	0.87	regulation of response to stimulus
GO:0048518	0.84	positive regulation of biological process
GO:0009966	0.82	regulation of signal transduction
GO:0048522	0.80	positive regulation of cellular process
GO:0060255	0.79	regulation of macromolecule metabolic process
GO:0031323	0.79	regulation of cellular metabolic process
GO:0080090	0.78	regulation of primary metabolic process
GO:0048584	0.77	positive regulation of response to stimulus
GO:0031325	0.76	positive regulation of cellular metabolic process
GO:0007165	0.76	signal transduction
GO:0032268	0.75	regulation of cellular protein metabolic process
GO:0019220	0.75	regulation of phosphate metabolic process
GO:0010604	0.75	positive regulation of macromolecule metabolic process
GO:0007166	0.75	cell surface receptor signaling pathway
GO:0007167	0.74	enzyme linked receptor protein signaling pathway
GO:0001934	0.74	positive regulation of protein phosphorylation
GO:1902531	0.73	regulation of intracellular signal transduction
GO:0009967	0.73	positive regulation of signal transduction
GO:0043408	0.72	regulation of MAPK cascade
GO:0032501	0.71	multicellular organismal process
GO:0044707	0.70	single-multicellular organism process
GO:0007169	0.69	transmembrane receptor protein tyrosine kinase signaling pathway
GO:0051270	0.68	regulation of cellular component movement
GO:0040012	0.68	regulation of locomotion
GO:0008284	0.68	positive regulation of cell proliferation
GO:0070374	0.67	positive regulation of ERK1 and ERK2 cascade
GO:0065008	0.67	regulation of biological quality
GO:0030335	0.67	positive regulation of cell migration
GO:0050790	0.66	regulation of catalytic activity
GO:0044093	0.66	positive regulation of molecular function
GO:0031954	0.66	positive regulation of protein autophosphorylation
GO:0050878	0.65	regulation of body fluid levels
GO:0048008	0.65	platelet-derived growth factor receptor signaling pathway
GO:0043406	0.65	positive regulation of MAP kinase activity
GO:0014068	0.65	positive regulation of phosphatidylinositol 3-kinase signaling
GO:0007596	0.64	blood coagulation

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044421	0.88	extracellular region part
GO:0005615	0.85	extracellular space
GO:0044464	0.64	cell part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2x58A	0.364	6.78	0.060	0.629	5.3.3.8 4.2.1.17 1.1.1.35	NA
2	0.060	1apqA	0.102	4.40	0.038	0.133	3.4.21.41 3.4.21.42	144,146
3	0.060	2g25A	0.387	6.93	0.031	0.664	1.2.4.1	NA
4	0.060	1yq2A	0.352	7.00	0.039	0.623	3.2.1.23	197,213
5	0.060	1l8aA	0.300	6.45	0.031	0.496	1.2.4.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.01	3kq4B	0.382	4.50	0.115	0.475	0.52	CA	complex1.pdb.gz	114,120,121

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.