D-I-TASSER Q9BT04

D-I-TASSER results for Q9BT04

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q9BT04
Protein Name: Protein fuzzy homolog
Gene Name: FUZ

Input Sequence in FASTA format

>Q9BT04 (418 residues)
MGEEGTGGTVHLLCLAASSGVPLFCRSSRGGAPARQQLPFSVIGSLNGVHMFGQNLEVQL
SSARTENTTVVWKSFHDSITLIVLSSEVGISELRLERLLQMVFGAMVLLVGLEELTNIRN
VERLKKDLRASYCLIDSFLGDSELIGDLTQCVDCVIPPEGSLLQEALSGFAEAAGTTFVS
LVVSGRVVAATEGWWRLGTPEAVLLPWLVGSLPPQTARDYPVYLPHGSPTVPHRLLTLTL
LPSLELCLLCGPSPPLSQLYPQLLERWWQPLLDPLRACLPLGPRALPSGFPLHTDILGLL
LLHLELKRCLFTVEPLGDKEPSPEQRRRLLRNFYTLVTSTHFPPEPGPPEKTEDEVYQAQ
LPRACYLVLGTEEPGTGVRLVALQLGLRRLLLLLSPQSPTHGLRSLATHTLHALTPLL

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q9BT04-D1	1-153	153		MGEEGTGGTVHLLCLAASSGVPLFCRSSRGGAPARQQLPFSVIGSLNGVHMFGQNLEVQLSSARTENTTVVWKSFHDSITLIVLSSEVGISELRLERLLQMVFGAMVLLVGLEELTNIRNVERLKKDLRASYCLIDSFLGDSELIGDLTQCVD
2	Q9BT04-D2	154-284	131		CVIPPEGSLLQEALSGFAEAAGTTFVSLVVSGRVVAATEGWWRLGTPEAVLLPWLVGSLPPQTARDYPVYLPHGSPTVPHRLLTLTLLPSLELCLLCGPSPPLSQLYPQLLERWWQPLLDPLRACLPLGPR
3	Q9BT04-D3	285-418	134		ALPSGFPLHTDILGLLLLHLELKRCLFTVEPLGDKEPSPEQRRRLLRNFYTLVTSTHFPPEPGPPEKTEDEVYQAQLPRACYLVLGTEEPGTGVRLVALQLGLRRLLLLLSPQSPTHGLRSLATHTLHALTPLL

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.69

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3spaA	0.41	6.67	0.040	0.651	model1_3spaA.pdb.gz
2	6r9tA	0.41	6.96	0.051	0.665	model1_6r9tA.pdb.gz
3	2vdcA	0.41	6.80	0.032	0.658	model1_2vdcA.pdb.gz
4	1bxrA	0.40	7.33	0.019	0.687	model1_1bxrA.pdb.gz
5	1pj6A	0.40	6.62	0.050	0.639	model1_1pj6A.pdb.gz
6	6npsA	0.40	6.65	0.049	0.629	model1_6npsA.pdb.gz
7	2hwgA	0.40	6.74	0.072	0.634	model1_2hwgA.pdb.gz
8	5h3zA	0.39	7.49	0.046	0.689	model1_5h3zA.pdb.gz
9	5gslA	0.39	6.84	0.072	0.636	model1_5gslA.pdb.gz
10	6lt0C	0.39	5.68	0.084	0.560	model1_6lt0C.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:1901363	0.70	heterocyclic compound binding
GO:0097159	0.70	organic cyclic compound binding
GO:0003676	0.65	nucleic acid binding
GO:0003723	0.63	RNA binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	1.00	cellular process
GO:0044699	0.95	single-organism process
GO:0044763	0.94	single-organism cellular process
GO:0016043	0.87	cellular component organization
GO:0070925	0.86	organelle assembly
GO:0032502	0.86	developmental process
GO:0065007	0.85	biological regulation
GO:0044767	0.85	single-organism developmental process
GO:0009653	0.85	anatomical structure morphogenesis
GO:0050789	0.84	regulation of biological process
GO:0001736	0.84	establishment of planar polarity
GO:0050794	0.83	regulation of cellular process
GO:0009966	0.78	regulation of signal transduction
GO:0048704	0.77	embryonic skeletal system morphogenesis
GO:0042384	0.77	cilium assembly
GO:0010172	0.77	embryonic body morphogenesis
GO:0008589	0.77	regulation of smoothened signaling pathway
GO:0001843	0.77	neural tube closure
GO:0048518	0.69	positive regulation of biological process
GO:0048522	0.68	positive regulation of cellular process
GO:0051239	0.67	regulation of multicellular organismal process
GO:0048856	0.67	anatomical structure development
GO:0048523	0.67	negative regulation of cellular process
GO:0044707	0.67	single-multicellular organism process
GO:2000026	0.66	regulation of multicellular organismal development
GO:0045595	0.66	regulation of cell differentiation
GO:0032879	0.66	regulation of localization
GO:0023057	0.66	negative regulation of signaling
GO:0022603	0.66	regulation of anatomical structure morphogenesis
GO:0010648	0.66	negative regulation of cell communication
GO:2000314	0.65	negative regulation of fibroblast growth factor receptor signaling pathway involved in neural plate anterior/posterior pattern formation
GO:0090301	0.65	negative regulation of neural crest formation
GO:0090090	0.65	negative regulation of canonical Wnt signaling pathway
GO:0045724	0.65	positive regulation of cilium assembly
GO:0035058	0.65	nonmotile primary cilium assembly
GO:0030336	0.65	negative regulation of cell migration
GO:0008285	0.65	negative regulation of cell proliferation
GO:0001942	0.65	hair follicle development

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0043226	1.00	organelle
GO:0043227	0.88	membrane-bounded organelle
GO:0044421	0.85	extracellular region part
GO:0031988	0.85	membrane-bounded vesicle
GO:0070062	0.84	extracellular exosome
GO:0044464	0.79	cell part
GO:0044424	0.62	intracellular part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3l4uA	0.365	7.35	0.033	0.629	3.2.1.20 3.2.1.3	NA
2	0.060	1aroP	0.364	7.13	0.036	0.605	2.7.7.6	NA
3	0.060	1kc7A	0.363	7.25	0.053	0.605	2.7.9.1	38,101
4	0.060	3gh4A	0.364	6.88	0.044	0.579	3.2.1.52	NA
5	0.060	1qbaA	0.363	6.93	0.057	0.601	3.2.1.52	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.01	1a9x2	0.355	6.49	0.049	0.548	0.43	III	complex1.pdb.gz	26,39,43,44,47
	2	0.01	1a9x1	0.356	6.47	0.049	0.548	0.46	III	complex2.pdb.gz	44,46,47,70,71

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.