D-I-TASSER Q99988

D-I-TASSER results for Q99988

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q99988
Protein Name: Growth/differentiation factor 15
Gene Name: GDF15

Input Sequence in FASTA format

>Q99988 (308 residues)
MPGQELRTVNGSQMLLVLLVLSWLPHGGALSLAEASRASFPGPSELHSEDSRFRELRKRY
EDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRL
HRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQL
ELHLRPQAARGRRRARARNGDHCPLGPGRCCRLHTVRASLEDLGWADWVLSPREVQVTMC
IGACPSQFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYNPMVLIQKTDTGVSLQTYDDL
LAKDCHCI

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q99988-D1	1-194	194		MPGQELRTVNGSQMLLVLLVLSWLPHGGALSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRR
2	Q99988-D2	195-308	114		ARARNGDHCPLGPGRCCRLHTVRASLEDLGWADWVLSPREVQVTMCIGACPSQFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYNPMVLIQKTDTGVSLQTYDDLLAKDCHCI

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.63

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4a01A	0.43	6.69	0.039	0.753	model1_4a01A.pdb.gz
2	6rc9A1	0.41	6.59	0.035	0.711	model1_6rc9A1.pdb.gz
3	4av3A	0.39	6.34	0.026	0.630	model1_4av3A.pdb.gz
4	6qq5A	0.39	6.43	0.052	0.672	model1_6qq5A.pdb.gz
5	5zrdA	0.39	6.12	0.047	0.636	model1_5zrdA.pdb.gz
6	5hkjA	0.39	5.63	0.054	0.604	model1_5hkjA.pdb.gz
7	7kf5A	0.38	6.34	0.027	0.627	model1_7kf5A.pdb.gz
8	4k0eA	0.38	6.46	0.037	0.640	model1_4k0eA.pdb.gz
9	4mt1A	0.38	6.38	0.022	0.623	model1_4mt1A.pdb.gz
10	6hraA	0.38	6.39	0.051	0.633	model1_6hraA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005102	0.98	receptor binding
GO:0005160	0.95	transforming growth factor beta receptor binding
GO:0005125	0.80	cytokine activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	0.99	regulation of cellular process
GO:0044699	0.96	single-organism process
GO:0009987	0.92	cellular process
GO:0080090	0.90	regulation of primary metabolic process
GO:0060255	0.90	regulation of macromolecule metabolic process
GO:0048522	0.90	positive regulation of cellular process
GO:0044763	0.90	single-organism cellular process
GO:0032502	0.90	developmental process
GO:0031323	0.90	regulation of cellular metabolic process
GO:0044767	0.89	single-organism developmental process
GO:0048583	0.88	regulation of response to stimulus
GO:0007165	0.88	signal transduction
GO:0031325	0.87	positive regulation of cellular metabolic process
GO:0010604	0.87	positive regulation of macromolecule metabolic process
GO:0023051	0.84	regulation of signaling
GO:0010646	0.84	regulation of cell communication
GO:0051246	0.83	regulation of protein metabolic process
GO:0048869	0.83	cellular developmental process
GO:0009967	0.83	positive regulation of signal transduction
GO:0045937	0.82	positive regulation of phosphate metabolic process
GO:0032270	0.82	positive regulation of cellular protein metabolic process
GO:0001932	0.82	regulation of protein phosphorylation
GO:0001934	0.81	positive regulation of protein phosphorylation
GO:0048856	0.80	anatomical structure development
GO:0043408	0.80	regulation of MAPK cascade
GO:0042981	0.77	regulation of apoptotic process
GO:0010862	0.76	positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0060395	0.72	SMAD protein signal transduction
GO:0048468	0.69	cell development
GO:0010556	0.66	regulation of macromolecule biosynthetic process
GO:0007166	0.65	cell surface receptor signaling pathway
GO:0007178	0.64	transmembrane receptor protein serine/threonine kinase signaling pathway
GO:0031326	0.60	regulation of cellular biosynthetic process
GO:2000112	0.59	regulation of cellular macromolecule biosynthetic process
GO:0051171	0.59	regulation of nitrogen compound metabolic process
GO:0010468	0.59	regulation of gene expression
GO:0006355	0.58	regulation of transcription, DNA-templated
GO:0006357	0.50	regulation of transcription from RNA polymerase II promoter

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044421	0.98	extracellular region part
GO:0005615	0.92	extracellular space
GO:0044464	0.70	cell part
GO:0044424	0.59	intracellular part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1zdlA	0.372	6.30	0.049	0.630	1.8.1.9	47
2	0.060	1coyA	0.350	6.06	0.051	0.571	1.1.3.6	NA
3	0.060	1lpsA	0.383	6.29	0.031	0.627	3.1.1.3	215
4	0.060	3b7wA	0.364	6.60	0.057	0.630	6.2.1.2	NA
5	0.060	3etcB	0.355	6.42	0.042	0.607	6.2.1.-	287

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1typA	0.373	6.43	0.027	0.636	0.11	UUU	complex1.pdb.gz	224,238,239,240,241
2	0.01	2v6oA	0.323	6.85	0.050	0.581	0.10	FAD	complex2.pdb.gz	9,11,14,15,18
3	0.01	1lpmA	0.375	6.24	0.022	0.610	0.11	MPA	complex3.pdb.gz	224,225,227
4	0.01	2wowC	0.376	6.48	0.024	0.640	0.24	GCG	complex4.pdb.gz	239,240,241,242
5	0.01	3rarA	0.376	6.36	0.026	0.620	0.19	IAN	complex5.pdb.gz	132,219,220,223

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.