D-I-TASSER Q96G46

D-I-TASSER results for Q96G46

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q96G46
Protein Name: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like
Gene Name: DUS3L

Input Sequence in FASTA format

>Q96G46 (650 residues)
MAEGTAEAPLENGGGGDSGAGALERGVAPIKRQYLTTKEQFHQFLEAKGQEKTCRETEVG
DPAGNELAEPEAKRIRLEDGQTADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDK
NRLCPSLIQESAAKCFFGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFA
GAHLRPEGQNLVQEELAARGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRRFSQGPT
PAAAVPEGTAAEGAPRQENCGAQQVPAGPGTSTPPSSPVRTCGPLTDEDVVRLRPCEKKR
LDIRGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCED
IFGVQLEGAFPDTMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQI
VRGMNQVLDVPLTVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQ
YIEECVQAASPMPLFGNGDILSFEDANRAMQTGVTGIMIARGALLKPWLFTEIKEQRHWD
ISSSERLDILRDFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINE
RPPYYLGRDYLETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAYK

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q96G46-D1	1-650	650		MAEGTAEAPLENGGGGDSGAGALERGVAPIKRQYLTTKEQFHQFLEAKGQEKTCRETEVGDPAGNELAEPEAKRIRLEDGQTADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQESAAKCFFGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLRPEGQNLVQEELAARGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRRFSQGPTPAAAVPEGTAAEGAPRQENCGAQQVPAGPGTSTPPSSPVRTCGPLTDEDVVRLRPCEKKRLDIRGKLYLAPLTTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPDTMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPLTVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQAASPMPLFGNGDILSFEDANRAMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAYK

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.3886

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6ei9A	0.44	2.31	0.237	0.462	model1_6ei9A.pdb.gz
2	2vcaA	0.44	5.90	0.067	0.579	model1_2vcaA.pdb.gz
3	1vhnA	0.43	2.36	0.272	0.452	model1_1vhnA.pdb.gz
4	3b0pA	0.41	3.31	0.209	0.459	model1_3b0pA.pdb.gz
5	3w9zA	0.40	2.48	0.237	0.429	model1_3w9zA.pdb.gz
6	2v5dA	0.39	6.38	0.052	0.523	model1_2v5dA.pdb.gz
7	1h7wD	0.37	5.48	0.062	0.454	model1_1h7wD.pdb.gz
8	1ep3A	0.33	3.54	0.129	0.368	model1_1ep3A.pdb.gz
9	3khjH	0.32	4.37	0.096	0.366	model1_3khjH.pdb.gz
10	4evzA	0.29	3.44	0.126	0.322	model1_4evzA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0016627	0.99	oxidoreductase activity, acting on the CH-CH group of donors
GO:0017150	0.98	tRNA dihydrouridine synthase activity
GO:1901363	0.54	heterocyclic compound binding
GO:0097159	0.54	organic cyclic compound binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.87	cellular process
GO:0008152	0.84	metabolic process
GO:0071704	0.82	organic substance metabolic process
GO:0044237	0.82	cellular metabolic process
GO:0044238	0.81	primary metabolic process
GO:0043170	0.80	macromolecule metabolic process
GO:0006807	0.80	nitrogen compound metabolic process
GO:0044260	0.79	cellular macromolecule metabolic process
GO:0034641	0.79	cellular nitrogen compound metabolic process
GO:0006139	0.78	nucleobase-containing compound metabolic process
GO:0090304	0.77	nucleic acid metabolic process
GO:0065007	0.77	biological regulation
GO:0050794	0.76	regulation of cellular process
GO:0016070	0.76	RNA metabolic process
GO:0006396	0.70	RNA processing
GO:0080090	0.69	regulation of primary metabolic process
GO:0034660	0.69	ncRNA metabolic process
GO:0031323	0.69	regulation of cellular metabolic process
GO:0034470	0.68	ncRNA processing
GO:0060255	0.67	regulation of macromolecule metabolic process
GO:0043412	0.66	macromolecule modification
GO:0008033	0.65	tRNA processing
GO:0048523	0.63	negative regulation of cellular process
GO:0006400	0.63	tRNA modification
GO:0044699	0.59	single-organism process
GO:0032268	0.59	regulation of cellular protein metabolic process
GO:0010605	0.59	negative regulation of macromolecule metabolic process
GO:0032269	0.58	negative regulation of cellular protein metabolic process
GO:2000112	0.57	regulation of cellular macromolecule biosynthetic process
GO:0051171	0.57	regulation of nitrogen compound metabolic process
GO:0010468	0.57	regulation of gene expression
GO:0044710	0.51	single-organism metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.99	cytoplasmic part
GO:0005829	0.98	cytosol

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1bglA	0.351	7.37	0.054	0.529	3.2.1.23	NA
2	0.060	2bfgA	0.359	5.77	0.050	0.472	3.2.1.37	NA
3	0.060	2epoB	0.385	6.04	0.066	0.508	3.2.1.52	NA
4	0.060	1px8A	0.356	5.76	0.050	0.469	3.2.1.37	NA
5	0.060	2qf7A	0.359	6.12	0.062	0.472	6.4.1.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.17	1vhnA	0.435	2.37	0.273	0.462	1.36	FMN	complex1.pdb.gz	310,311,312,313,315,336,365,393,435,465,473,497,498,499,500,520,521
2	0.01	2vurA	0.388	6.20	0.043	0.520	0.45	YX1	complex2.pdb.gz	312,395,438,466,497,499,520
3	0.01	2wzhA	0.389	5.80	0.060	0.506	0.42	NGO	complex3.pdb.gz	311,312,393,394,433,464,496,498,519
4	0.01	1ps9A	0.389	5.37	0.111	0.480	0.43	SF4	complex4.pdb.gz	315,316,317,320,333
5	0.01	2cbjB	0.392	5.92	0.055	0.514	0.52	OAN	complex5.pdb.gz	310,311,312,392,393,466,495,497,518
6	0.01	1o94B	0.402	5.04	0.100	0.485	0.45	SF4	complex6.pdb.gz	313,314,315,316,317,318

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.