D-I-TASSER Q96AV8

D-I-TASSER results for Q96AV8

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q96AV8
Protein Name: Transcription factor E2F7
Gene Name: E2F7

Input Sequence in FASTA format

>Q96AV8 (911 residues)
MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKF
TPERNPITPVKFVDRQQAEPWTPTANLKMLISAASPDIRDREKKKGLFRPIENKDDAFTD
SLQLDVVGDSAVDEFEKQRPSRKQKSLGLLCQKFLARYPSYPLSTEKTTISLDEVAVSLG
VERRRIYDIVNVLESLHLVSRVAKNQYGWHGRHSLPKTLRNLQRLGEEQKYEEQMAYLQQ
KELDLIDYKFGERKKDGDPDSQEQQLLDFSEPDCPSSSANSRKDKSLRIMSQKFVMLFLV
SKTKIVTLDVAAKILIEESQDAPDHSKFKTKVRRLYDIANVLTSLALIKKVHVTEERGRK
PAFKWIGPVDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERI
QRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPV
APFPVLSVDPEYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPAL
LAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEER
KPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLP
AAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPAGLNGFNV
LLSGSQTPPTVGPSSGQLPSFSVPCMVLPSPPLGPFPVLYSPAMPGPVSSTLGALPNTGP
VNFSLPGLGSIAQLLVGPTAVVNPKSSTLPSADPQLQSQPSLNLSPVMSRSHSVVQQPES
PVYVGHPVSVVKLHQSPVPVTPKSIQRTHRETFFKTPGSLGDPVLKRRERNQSRNTSSAQ
RRLEIPSGGAD

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q96AV8-D1	1-911	911		MEVNCLTLKDLISPRQPRLDFAVEDGENAQKENIFVDRSRMAPKTPIKNEPIDLSKQKKFTPERNPITPVKFVDRQQAEPWTPTANLKMLISAASPDIRDREKKKGLFRPIENKDDAFTDSLQLDVVGDSAVDEFEKQRPSRKQKSLGLLCQKFLARYPSYPLSTEKTTISLDEVAVSLGVERRRIYDIVNVLESLHLVSRVAKNQYGWHGRHSLPKTLRNLQRLGEEQKYEEQMAYLQQKELDLIDYKFGERKKDGDPDSQEQQLLDFSEPDCPSSSANSRKDKSLRIMSQKFVMLFLVSKTKIVTLDVAAKILIEESQDAPDHSKFKTKVRRLYDIANVLTSLALIKKVHVTEERGRKPAFKWIGPVDFSSSDEELVDVSASVLPELKRETYGQIQVCAKQKLARHGSFNTVQASERIQRKVNSEPSSPYREEQGSGGYSLEIGSLAAVYRQKIEDNSQGKAFASKRVVPPSSSLDPVAPFPVLSVDPEYCVNPLAHPVFSVAQTDLQAFSMQNGLNGQVDVSLASAASAVESLKPALLAGQPLVYVPSASLFMLYGSLQEGPASGSGSERDDRSSEAPATVELSSAPSAQKRLCEERKPQEEDEPATKRQSREYEDGPLSLVMPKKPSDSTDLASPKTMGNRASIPLKDIHVNGQLPAAEEISGKATANSLVSSEWGNPSRNTDVEKPSKENESTKEPSLLQYLCVQSPAGLNGFNVLLSGSQTPPTVGPSSGQLPSFSVPCMVLPSPPLGPFPVLYSPAMPGPVSSTLGALPNTGPVNFSLPGLGSIAQLLVGPTAVVNPKSSTLPSADPQLQSQPSLNLSPVMSRSHSVVQQPESPVYVGHPVSVVKLHQSPVPVTPKSIQRTHRETFFKTPGSLGDPVLKRRERNQSRNTSSAQRRLEIPSGGAD

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5951

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6ez8A	0.31	8.05	0.033	0.446	model1_6ez8A.pdb.gz
2	7b52A	0.30	8.91	0.043	0.472	model1_7b52A.pdb.gz
3	2vsqA	0.29	8.02	0.041	0.429	model1_2vsqA.pdb.gz
4	6edoA	0.28	8.99	0.032	0.452	model1_6edoA.pdb.gz
5	3qmzA	0.28	8.60	0.034	0.440	model1_3qmzA.pdb.gz
6	6zwmA	0.28	8.90	0.034	0.452	model1_6zwmA.pdb.gz
7	5x6oC	0.28	8.66	0.054	0.436	model1_5x6oC.pdb.gz
8	7jgdA	0.28	8.79	0.028	0.437	model1_7jgdA.pdb.gz
9	5m59A	0.27	8.95	0.029	0.434	model1_5m59A.pdb.gz
10	1wygA	0.27	9.04	0.033	0.436	model1_1wygA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003700	0.98	transcription factor activity, sequence-specific DNA binding
GO:1901363	0.90	heterocyclic compound binding
GO:0097159	0.90	organic cyclic compound binding
GO:0003677	0.87	DNA binding
GO:0003714	0.83	transcription corepressor activity
GO:0044212	0.73	transcription regulatory region DNA binding
GO:0001047	0.70	core promoter binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	1.00	regulation of cellular process
GO:0009987	0.98	cellular process
GO:0080090	0.97	regulation of primary metabolic process
GO:0060255	0.97	regulation of macromolecule metabolic process
GO:0031323	0.97	regulation of cellular metabolic process
GO:2000112	0.96	regulation of cellular macromolecule biosynthetic process
GO:0019219	0.96	regulation of nucleobase-containing compound metabolic process
GO:0010468	0.95	regulation of gene expression
GO:0006355	0.94	regulation of transcription, DNA-templated
GO:0051726	0.93	regulation of cell cycle
GO:0044699	0.93	single-organism process
GO:0048523	0.92	negative regulation of cellular process
GO:0048522	0.92	positive regulation of cellular process
GO:0044763	0.91	single-organism cellular process
GO:0031325	0.91	positive regulation of cellular metabolic process
GO:0010604	0.91	positive regulation of macromolecule metabolic process
GO:0045935	0.90	positive regulation of nucleobase-containing compound metabolic process
GO:0044767	0.90	single-organism developmental process
GO:0031328	0.90	positive regulation of cellular biosynthetic process
GO:0010605	0.90	negative regulation of macromolecule metabolic process
GO:0010557	0.90	positive regulation of macromolecule biosynthetic process
GO:0006357	0.90	regulation of transcription from RNA polymerase II promoter
GO:2000113	0.89	negative regulation of cellular macromolecule biosynthetic process
GO:0045934	0.89	negative regulation of nucleobase-containing compound metabolic process
GO:0048869	0.88	cellular developmental process
GO:0010948	0.88	negative regulation of cell cycle process
GO:0045892	0.87	negative regulation of transcription, DNA-templated
GO:0045787	0.87	positive regulation of cell cycle
GO:0032875	0.87	regulation of DNA endoreduplication
GO:0090068	0.86	positive regulation of cell cycle process
GO:0048646	0.86	anatomical structure formation involved in morphogenesis
GO:0030855	0.86	epithelial cell differentiation
GO:0000122	0.86	negative regulation of transcription from RNA polymerase II promoter
GO:0051782	0.85	negative regulation of cell division
GO:0070365	0.84	hepatocyte differentiation
GO:0060718	0.84	chorionic trophoblast cell differentiation
GO:0032877	0.84	positive regulation of DNA endoreduplication
GO:0032466	0.84	negative regulation of cytokinesis
GO:0002040	0.84	sprouting angiogenesis
GO:0022402	0.72	cell cycle process
GO:1903047	0.68	mitotic cell cycle process
GO:0048856	0.68	anatomical structure development
GO:0044702	0.67	single organism reproductive process
GO:0050896	0.66	response to stimulus
GO:0048513	0.66	animal organ development
GO:0003006	0.66	developmental process involved in reproduction
GO:0060707	0.65	trophoblast giant cell differentiation
GO:0042127	0.65	regulation of cell proliferation
GO:0001890	0.65	placenta development
GO:0007346	0.63	regulation of mitotic cell cycle
GO:0007165	0.63	signal transduction
GO:1901990	0.62	regulation of mitotic cell cycle phase transition
GO:0072331	0.62	signal transduction by p53 class mediator
GO:0045930	0.62	negative regulation of mitotic cell cycle
GO:0008285	0.62	negative regulation of cell proliferation
GO:0006974	0.62	cellular response to DNA damage stimulus
GO:2000045	0.61	regulation of G1/S transition of mitotic cell cycle
GO:1901991	0.61	negative regulation of mitotic cell cycle phase transition
GO:0000083	0.61	regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:2000134	0.60	negative regulation of G1/S transition of mitotic cell cycle
GO:0071930	0.60	negative regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0030330	0.60	DNA damage response, signal transduction by p53 class mediator
GO:0045893	0.50	positive regulation of transcription, DNA-templated

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0043226	0.75	organelle
GO:0043229	0.74	intracellular organelle
GO:0043227	0.73	membrane-bounded organelle
GO:0043231	0.72	intracellular membrane-bounded organelle
GO:0005634	0.71	nucleus
GO:0044446	0.63	intracellular organelle part
GO:0044428	0.57	nuclear part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1w36C	0.238	9.41	0.042	0.399	3.1.11.5	NA
2	0.060	3f2bA	0.237	8.43	0.055	0.362	2.7.7.7	NA
3	0.060	3iydD	0.228	8.67	0.033	0.352	2.7.7.6	NA
4	0.060	3lxuX	0.265	8.62	0.033	0.413	3.4.14.10	NA
5	0.060	2cqsA	0.199	8.80	0.034	0.314	2.4.1.20	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.