D-I-TASSER Q8WZ60

D-I-TASSER results for Q8WZ60

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q8WZ60
Protein Name: Kelch-like protein 6
Gene Name: KLHL6

Input Sequence in FASTA format

>Q8WZ60 (621 residues)
MLMAGQRGAWTMGDVVEKSLEGPLAPSTDEPSQKTGDLVEILNGEKVKFDDAGLSLILQN
GLETLRMENALTDVILCVDIQEFSCHRVVLAAASNYFRAMFCNDLKEKYEKRIIIKGVDA
ETMHTLLDYTYTSKALITKQNVQRVLEAANLFQFLRMVDACASFLTEALNPENCVGILRL
ADTHSLDSLKKQVQSYIIQNFVQILNSEEFLDLPVDTLHHILKSDDLYVTEEAQVFETVM
SWVRHKPSERLCLLPYVLENVRLPLLDPWYFVETVEADPLIRQCPEVFPLLQEARMYHLS
GNEIISERTKPRMHEFQSEVFMIIGGCTKDERFVAEVTCLDPLRRSRLEVAKLPLTEHEL
ESENKKWVEFACVTLKNEVYISGGKETQHDVWKYNSSINKWIQIEYLNIGRWRHKMVVLG
GKVYVIGGFDGLQRINNVETYDPFHNCWSEAAPLLVHVSSFAATSHKKKLYVIGGGPNGK
LATDKTQCYDPSTNKWSLKAAMPVEAKCINAVSFRDRIYVVGGAMRALYAYSPLEDSWCL
VTQLSHERASCGIAPCNNRLYITGGRDEKNEVIATVLCWDPEAQKLTEECVLPRGVSHHG
SVTIRKSYTHIRRIVPGAVSV

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q8WZ60-D1	1-207	207		MLMAGQRGAWTMGDVVEKSLEGPLAPSTDEPSQKTGDLVEILNGEKVKFDDAGLSLILQNGLETLRMENALTDVILCVDIQEFSCHRVVLAAASNYFRAMFCNDLKEKYEKRIIIKGVDAETMHTLLDYTYTSKALITKQNVQRVLEAANLFQFLRMVDACASFLTEALNPENCVGILRLADTHSLDSLKKQVQSYIIQNFVQILNS
2	Q8WZ60-D2	208-312	105		EEFLDLPVDTLHHILKSDDLYVTEEAQVFETVMSWVRHKPSERLCLLPYVLENVRLPLLDPWYFVETVEADPLIRQCPEVFPLLQEARMYHLSGNEIISERTKPR
3	Q8WZ60-D3	313-621	309		MHEFQSEVFMIIGGCTKDERFVAEVTCLDPLRRSRLEVAKLPLTEHELESENKKWVEFACVTLKNEVYISGGKETQHDVWKYNSSINKWIQIEYLNIGRWRHKMVVLGGKVYVIGGFDGLQRINNVETYDPFHNCWSEAAPLLVHVSSFAATSHKKKLYVIGGGPNGKLATDKTQCYDPSTNKWSLKAAMPVEAKCINAVSFRDRIYVVGGAMRALYAYSPLEDSWCLVTQLSHERASCGIAPCNNRLYITGGRDEKNEVIATVLCWDPEAQKLTEECVLPRGVSHHGSVTIRKSYTHIRRIVPGAVSV

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.62

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	7lhtA	0.47	6.34	0.075	0.655	model1_7lhtA.pdb.gz
2	4yy8A	0.47	2.94	0.218	0.502	model1_4yy8A.pdb.gz
3	3ei2A	0.46	6.83	0.049	0.662	model1_3ei2A.pdb.gz
4	2dyhA	0.45	1.44	0.225	0.457	model1_2dyhA.pdb.gz
5	2uvkB	0.39	1.93	0.210	0.409	model1_2uvkB.pdb.gz
6	2eidA	0.36	4.65	0.089	0.440	model1_2eidA.pdb.gz
7	2wozA	0.36	3.06	0.179	0.387	model1_2wozA.pdb.gz
8	3hxjA	0.29	4.48	0.065	0.345	model1_3hxjA.pdb.gz
9	4ascA1	0.21	2.53	0.170	0.227	model1_4ascA1.pdb.gz
10	2vseA2	0.17	6.03	0.042	0.235	model1_2vseA2.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.97	catalytic activity
GO:0019787	0.96	ubiquitin-like protein transferase activity
GO:0004842	0.94	ubiquitin-protein transferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.96	cellular process
GO:0008152	0.79	metabolic process
GO:0071704	0.78	organic substance metabolic process
GO:0044237	0.78	cellular metabolic process
GO:0044260	0.77	cellular macromolecule metabolic process
GO:0044238	0.77	primary metabolic process
GO:0019538	0.71	protein metabolic process
GO:0006464	0.70	cellular protein modification process
GO:0032446	0.68	protein modification by small protein conjugation
GO:0016567	0.66	protein ubiquitination
GO:0065007	0.56	biological regulation
GO:0050789	0.52	regulation of biological process
GO:0042787	0.52	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0050794	0.50	regulation of cellular process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0043234	0.98	protein complex
GO:0031461	0.97	cullin-RING ubiquitin ligase complex
GO:0031463	0.94	Cul3-RING ubiquitin ligase complex
GO:0043226	0.80	organelle
GO:0043229	0.79	intracellular organelle
GO:0043227	0.70	membrane-bounded organelle
GO:0043231	0.69	intracellular membrane-bounded organelle
GO:0044422	0.67	organelle part
GO:0044446	0.63	intracellular organelle part
GO:0005737	0.63	cytoplasm
GO:0005634	0.62	nucleus
GO:0044444	0.50	cytoplasmic part

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1vcuB	0.383	3.51	0.062	0.432	3.2.1.18	NA
2	0.060	2vk6A	0.385	3.88	0.087	0.441	3.2.1.18	NA
3	0.060	2madH	0.371	4.90	0.081	0.462	1.4.99.3	NA
4	0.060	1a4gA	0.367	4.29	0.060	0.435	3.2.1.18	NA
5	0.060	1kb0A	0.370	5.96	0.104	0.494	1.1.99.-	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2zw9A	0.391	3.46	0.062	0.432	0.11	SAM	complex1.pdb.gz	339,520,521,522,565,566
2	0.01	2hye0	0.383	5.67	0.046	0.499	0.22	III	complex2.pdb.gz	387,388,458,499
3	0.01	3i7nA	0.423	7.27	0.035	0.644	0.28	III	complex3.pdb.gz	412,415,459,506,510
4	0.01	3i8cA	0.416	7.41	0.037	0.641	0.10	III	complex4.pdb.gz	463,475,476
5	0.01	2b5l0	0.378	5.81	0.047	0.498	0.20	III	complex5.pdb.gz	387,414,454,458,475,483,484,499,500,501
6	0.01	1w0oA	0.389	3.91	0.065	0.444	0.19	SIA	complex6.pdb.gz	426,428,487,488
7	0.01	3i8eA	0.419	7.33	0.032	0.639	0.15	III	complex7.pdb.gz	459,461,573
8	0.01	3i8eB	0.417	7.44	0.037	0.646	0.23	III	complex8.pdb.gz	389,414,461
9	0.01	2zwaB	0.394	3.57	0.065	0.436	0.10	SAH	complex9.pdb.gz	388,427,455

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.