D-I-TASSER Q8WU20

D-I-TASSER results for Q8WU20

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q8WU20
Protein Name: Fibroblast growth factor receptor substrate 2
Gene Name: FRS2

Input Sequence in FASTA format

>Q8WU20 (508 residues)
MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYL
CLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVER
NNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGE
ESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDP
QILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPS
VNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYENLPSLPPVWE
ARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKIEYSRRRDCTPTVF
NFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTA
AMSNLQKALPRDDGTSRKTRHNSTDLPM

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q8WU20-D1	1-508	508		MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPSVNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYENLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKIEYSRRRDCTPTVFNFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSNLQKALPRDDGTSRKTRHNSTDLPM

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.4939

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6rc9A	0.39	7.45	0.034	0.638	model1_6rc9A.pdb.gz
2	6rutB	0.38	7.49	0.036	0.632	model1_6rutB.pdb.gz
3	5yotA	0.37	7.63	0.059	0.614	model1_5yotA.pdb.gz
4	5i6fA	0.36	7.66	0.040	0.606	model1_5i6fA.pdb.gz
5	5z87A	0.36	7.77	0.045	0.612	model1_5z87A.pdb.gz
6	6f2pA	0.36	7.72	0.046	0.614	model1_6f2pA.pdb.gz
7	6w98A	0.36	7.29	0.058	0.573	model1_6w98A.pdb.gz
8	5dotA	0.35	7.23	0.049	0.563	model1_5dotA.pdb.gz
9	6um1A	0.35	7.47	0.040	0.579	model1_6um1A.pdb.gz
10	5jp0A	0.35	7.64	0.056	0.595	model1_5jp0A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005102	0.89	receptor binding
GO:0005104	0.86	fibroblast growth factor receptor binding
GO:0004871	0.66	signal transducer activity
GO:0019899	0.60	enzyme binding
GO:0005068	0.59	transmembrane receptor protein tyrosine kinase adaptor activity
GO:0005126	0.58	cytokine receptor binding
GO:0005168	0.57	neurotrophin TRKA receptor binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	1.00	regulation of cellular process
GO:0007165	0.88	signal transduction
GO:0007166	0.84	cell surface receptor signaling pathway
GO:0007169	0.82	transmembrane receptor protein tyrosine kinase signaling pathway
GO:0044699	0.79	single-organism process
GO:0009987	0.79	cellular process
GO:0008543	0.76	fibroblast growth factor receptor signaling pathway
GO:0044763	0.75	single-organism cellular process
GO:0048583	0.71	regulation of response to stimulus
GO:0048518	0.71	positive regulation of biological process
GO:0023051	0.70	regulation of signaling
GO:0010646	0.70	regulation of cell communication
GO:0048522	0.69	positive regulation of cellular process
GO:0009966	0.69	regulation of signal transduction
GO:0080090	0.68	regulation of primary metabolic process
GO:0060255	0.68	regulation of macromolecule metabolic process
GO:0031323	0.68	regulation of cellular metabolic process
GO:0048584	0.67	positive regulation of response to stimulus
GO:0032268	0.66	regulation of cellular protein metabolic process
GO:0031325	0.66	positive regulation of cellular metabolic process
GO:0010604	0.66	positive regulation of macromolecule metabolic process
GO:0009967	0.66	positive regulation of signal transduction
GO:1902531	0.65	regulation of intracellular signal transduction
GO:0001934	0.65	positive regulation of protein phosphorylation
GO:0043410	0.64	positive regulation of MAPK cascade
GO:0043085	0.64	positive regulation of catalytic activity
GO:0043405	0.63	regulation of MAP kinase activity
GO:0032147	0.63	activation of protein kinase activity
GO:0008152	0.54	metabolic process
GO:0071704	0.52	organic substance metabolic process
GO:0044238	0.51	primary metabolic process
GO:0044237	0.51	cellular metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0016020	0.90	membrane
GO:0005886	0.89	plasma membrane
GO:0044424	0.87	intracellular part
GO:0030054	0.59	cell junction
GO:0005911	0.58	cell-cell junction
GO:0044444	0.56	cytoplasmic part
GO:0043226	0.55	organelle
GO:0043229	0.53	intracellular organelle
GO:0043227	0.52	membrane-bounded organelle
GO:0043231	0.51	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2e1qA	0.315	7.62	0.017	0.516	1.17.3.2 1.17.1.4	NA
2	0.060	3c46B	0.329	8.14	0.037	0.577	2.7.7.6	NA
3	0.060	1kblA	0.314	7.05	0.045	0.498	2.7.9.1	NA
4	0.060	3eqoA	0.311	7.64	0.040	0.520	3.2.1.58	NA
5	0.060	3dy5A	0.317	7.94	0.030	0.553	1.13.11.40 4.2.1.92	337

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2ckjB	0.287	7.39	0.027	0.461	0.60	FES	complex1.pdb.gz	76,77,82,84,85
2	0.01	1v97A	0.347	7.19	0.041	0.557	0.51	FES	complex2.pdb.gz	33,34,78,79,80,81
3	0.01	2ckjC	0.296	7.78	0.044	0.498	0.54	FES	complex3.pdb.gz	36,37,48,61

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.