D-I-TASSER Q8TDG4-D1

D-I-TASSER results for Q8TDG4-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q8TDG4 (1101 residues)
MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLP
VEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFI
AQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGY
EGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCI
EQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEIN
VAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLP
TSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKG
RFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMT
LAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSK
AENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKF
LSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYS
TGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESI
LILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGT
FFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKG
TIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAE
QNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMP
RGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVL
EGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEV
EELLRLPSDFPGAVASSTDKA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 840 860 880 900 920 940 960 980 1000 1020 1040 1060 1080 1100 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLPVEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFIAQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGYEGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCIEQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEINVAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKGRFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESILILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGTFFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKGTIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAEQNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMPRGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVLEGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEVEELLRLPSDFPGAVASSTDKA
Prediction	CCCCCCCCSSSSSSCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCSSSCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCSSSSCCCCCCHHHHHHHHHHHHHHHCCCCSSSSCCHHHHHHHHHHHHHHHHHHCCCSSSSSSCCCCCCCCHHHCCCCCSSSSCHHHHHHHHHCCCCCCCCCCSCSSSSSCSSSCCCCCCCHHHHHHHHHHHHHCCCCSSSSSCCCCCCHHHHHHHCCCCCSCCCCCCCCCSSSSSSCCCSSSCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCSSSSSCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCSSSCCCCCHHHHHHHHHHHHHCCCSSSSSCCHHHHCCCCCCSSSSSSCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCSSSSSSCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
Confidence	997777522455412457765454457887233377777741555422420102456675446743345651232147887651466653220356666786067750554332342157665578888750235664223555510344443021111102222121112211111112322345442134314114566665300112210111110002211220110123452012033334454321122211222110123320234578877899885123326764322212689999998666656765999999999963475335558999974322216996799816887318999999999999569918998485999999999999999870976978844688786333223686999856899999970455330020278999644556899953789999999998267854999748989999999871861014762899964899817833304652022233445431122302210351489999999970897799972999999999999998775156535677799999998623555469999999738366519999899999999999099529995541675157883399990475378779999996642235888877775599981873199999998569986211234667999999999999857678999999999973999997523435368999999999999985566544444432035630110368999997077899999999998642122078899999834542100124607999999998398678888876087689999885066555405899999999999999998089999999996998789999999999999999999998252677999999999999936517889873358998999999999099999999859999999998506889999999999878889999999999999727752001100232129
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 800 820 840 860 880 900 920 940 960 980 1000 1020 1040 1060 1080 1100 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLPVEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFIAQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGYEGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCIEQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEINVAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKGRFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESILILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGTFFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKGTIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAEQNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMPRGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVLEGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEVEELLRLPSDFPGAVASSTDKA
Prediction	755324403431424444454244224434446354465465454444443444325344441435325434423324344434422443345442524432434411442331444412430442354324246344444444445224541444242433441452555544554461553444644421340144323424414544553444445454354544544555344544544455655454444454345464244434542445452522643444426634233203113343554643113024014402510341231430230022003341034431000001101000000000000001434220000000100011004302620461401000001224324444414400000001300000011013442043010000000000116400000000000012114410000000102204200410403204442000102100003430132444232433133113232343143122210120033027520000000114201400420041045422554245224004303514554324202400320000000001440141004005522000000000000001010000001023324430210001000120333222230000000246225202510444044031302440331013000000012213225102300310000002432353440241034014202421212232122122224220112210100021002141022004202412331203100000000130421441433132024004504543440242141233102220444314452233002100000001000441204200520401200010002000100300030031024033034004302400330034001200404603340021015120400430371426302400561344104401530442144205403530450151244044114544668
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780                 800                 820                 840                 860                 880                 900                 920                 940                 960                 980                1000                1020                1040                1060                1080                1100

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SS
Seq

CCCCCCCCSSSSSSCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHCCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCSSSCCCCCCCSSSCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCSSSSCCCCCCHHHHHHHHHHHHHHHCCCCSSSSCCHHHHHHHHHHHHHHHHHHCCCSSSSSSCCCCCCCCHHHCCCCCSSSSCHHHHHHHHHCCCCCCCCCCSCSSSSSCSSSCCCCCCCHHHHHHHHHHHHHCCCCSSSSSCCCCCCHHHHHHHCCCCCSCCCCCCCCCSSSSSSCCCSSSCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHCCCCSSSSSCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCSSSCCCCCHHHHHHHHHHHHHCCCSSSSSCCHHHHCCCCCCSSSSSSCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCSSSSSSCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCSCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLPVEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFIAQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGYEGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCIEQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEINVAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLPTSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKGRFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMTLAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSKAENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKFLSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYSTGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESILILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGTFFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKGTIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAEQNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMPRGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVLEGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEVEELLRLPSDFPGAVASSTDKA

4f91B

0.14

0.12

4.11

1.08

EigenThreader

DKNNLVKYD---------KKTGNFQVTELGRIASHYYITNDTVQTYNQLL----KPTLSEIELFRVFSLSSE---FKNITVREEEKLELQKLLERVPIPVKESIEEQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKTRSITPDFQWDEKVHGSVDSEVKYAQDEFEPLPPRWLSCFRHLIL---------------------PEKYPPPTE------------------LLDLQPLPVSALRNSAFESLYQDKFPF--FNPIQTQVFNTVYNSDDNV-FVGAPTGSGKTICAEFAILRMLLQSEGRCVYITPMEALAEQVYMDWYEKFQLNKKVVLL---TGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSHPNVRPVPLELHIQG--FN-----------------ISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAAD---IQRKDLIPYLE-------KLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMTQYYKIHDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHC-----MHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLSELVEQTLSDLEQSKCISIEDEM--------DVAPLNLGMIAAYYYINYTTIELFSMSL---NAKTKVRGLIEIISNAAEY----ENIPIR----------HHEDNLLRQLAQKVP--------HKLNNPKFND---PHVKTNLLLQAHLSRMQL-------SAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVEERNALLQ---------LTDSQIADVARFCNRY----------------PNPVIAPLFPQKREEGW

5agaA

0.37

0.24

7.21

3.74

SPARKS-K

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKLLLANWGLPKAVLEKYHS-FGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTS---PSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREF--------EPMLQVKGDED-HVVSLCYETIDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQLEQKELLEVMDQLRRLPSG-LDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFL-----AASMG-----AIEACVMWLLENEFIQSTEEGK-------VYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMF-EDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTEQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLG-WHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNTEREAAALIVEEARMILQQDL----------------------------

4bgdA

0.11

0.10

3.42

1.02

EigenThreader

ELENDVIEAT-------------------DLGNIASSFYINHASMDVYNRELDEHTTQIDLFRIFSMSEEFKYVSVRYEEKRELKQLLEKAPIPIREDIDDQSYFSQLKFEGFALNSDIVFIHQNAGRLLRAMFEICLKRGWGHPTRMLLNLCKSATTKMWPTNC----------PLRQFKTCPTVPWGDYLQLETSEKYGKQVYDLLKRFPKTRSIIADWIWDMNVHGSLTDGDSPDIVGHKQHNQNNLPPENWWHSVSFNGFK--------------LPKKFPPPT-----------------PLLENISISTSELGNDDFSEVFE----FKTFNKIQSQVFESLYNSNDSV-FVGSGKGTGKTAMAELALLNHWRQNKGRAVYINPSGEKIDFLLSDWNKRFSAGGKIINK--LGNDPSLNLKLLAKSHVLLATPVQFELLSRRWRQRKNIQSLELMIYDDAHEISQGVYGAVYETLISRMIFIAKKIRFVCLSNCLANARDFGEWAGMTKSNSPSERIEPLEINIQSFK----DVE-----------HISFNFSMLQ---MAFEASAAAAGNRNSSSVFLPSRKDCMEVASAFMKFSKAVPYIE-----------------KLTDGHLRAPLKHGVGILYKGMASNDERIVKRLYEYGAVSVLLISKDCSAFACKTDEVIILGTNDGAEHPYTINELLEMVG-LASGNDSMAGKVLILTSHNMKAYYKKFL---IEPLPTESY--LQYIIHDTLNNEIANSIIQSKQDCVDWFTYSYFYRRIHVNPSYYGLSNLVETCLNDLVESSFIEIDDT-------TEIISTLSNGLIASHYGVSFFTIQSFVSSLS----NTSTLKNMLYVLSTAVEFESVPLRKG-----------DRALLVKLSKRLPLR--------FPEHTSSGSVSFKVFLLLQAYFS----------------RLENDLKDILEKVVPLINVVVDILSANGYLNATTAMDLAQMLIQGVWDVDNPLRQI--PHFNNKILEKCKEINVETVYDIMALEDEERDEILTLTDSQLAQVAAFVNNYP----------------ENLQVTSEKYPFDKLES

2zjaA

0.27

0.17

5.16

1.24

MapAlign

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RVDELRVDERIKSTLKER-GIESFYPPQAEALKS-GILEGKNALISIPTASGKTLIAEIAMVHRILTQGGKAVYIVPLKALAEEKFQEFQ-DWEKIGLRVAMATGDYDSK--DEWLGKYDIIIATAEKFDSLLRHG--SSWIKDVKILVADEIHLIGSRDRGATLEVILAHML---GKAQIIGLSATIGNPEELAEWLNAELIVSDWRPVKLRRGVFYQGFVTWE-------DGSIDRFSS-------------WEELVYDAIRKKGALIFVNMRRKAERVALELSKKVKSLLTKPEIRALNELADSLE---ENPTNEKLAKAIRGGVAFHHAGLGRDERVLVEENFRKGIIKAVVATPTLSAGINTPAFRVIIRDIWRYMERIPIIEVHQMLGRAGRPKYDEVGEGIIVSTSDDPREVMHYIFGKPEKLFSQLS--NESNLRSQVLALIATFGYSTVEEILKFISNTFYAYQ---RKDTYSLEEKIRNILYFLLENEFIEIS--------LEDKIRPLSLGIRTAKLYIDPYTAKMFKDKMEEVVKDPNPIGIFHLISLTPDI-TP--FN--YKREFERLEEE---YYEFKDRLYF-DDPYISG-----YDPYLERKFFRAFKTALVLLAWINEVPEGEIVEKYSVEPGDIYRIVETAEWLVYSLKEIAKVL-GAYEIVDYLETLRVRVKYGIREELIPLMQLPLVGRRRARALYNSGFRSIEDISQARPEELLKIE-GIGVKTVEAIFKFL------------------------------------

5m59A

0.12

0.08

2.94

1.00

DEthreader

-----------DDGNTMREINDLDGEPYGIKVSELTGDRQLTK-------TPE-------KWDVITRKAT---------D---------------------------------------------------------------------------------------------------------------------ISYTNLVRLIIIDEI------R--V---I----------GEPVRIIGLVASFLRVDLSLAEILGNV---------------------------------------------LDLQPLPVSALKAKDYAALYP--NWQQFNKIQTQTFN-SLYNTDNNVLVAAPTGSGKTVCAEFALLRHWAKKAGRAVYIAPFQELVDLRFQDWQKRLSHLGKEIVKLTGETTTDLKL-LE-QGDLILATPLQWDVLSRQWKRRKNVQTVELFIADDLHMLGG-QMGYIYEIVVSRMHFIRTPMRIVGLSVSLANARDIGEWIDADIYNFSPVRPVPLELHIQSYT-------------------------LMLAMAKPTYLAITQLSPDQPAIVFVPSRKQTRATARDLLTCEDRFLHVEVDQIRKLLDH--V-----Q-EEALAEALSHGVGYYHEALSQSDKRIVKHLYNNGAIQVLIASRDVCWELDFTAHLVVVMGTQFRYIDYPLSEVLQMFGKALQPSKDGRSRGVLMLP-AVKREYYKKFLNEALPVESHL----HNFLPDAFVTEISTKMIESGEDAINWATFTYFYRRLLAPPTGSYLSDLVETTLKQLSDARIIEM-DEDE------GTVAPLNAAMIAAYYNISYMTMEMF-LLSLSH-KSKLRTILEIVTAATEFESIQ-TRRHEEGILKRIY-DHV-PV--KMN--NP----V---W----------D-S-AHFKAFVLVQAHFSRM---NLP---IDLAKDQE-VILQKILSLLSAIVDILSSEGHLNALNAMEMSQMVVQAMWDSPLKQIP------NF-TPEVVKVANIFMQMNNPNYASLVKDLG--L---T-QAQLAQAANFTNN---DI-------------------------

2zjaA

0.26

0.17

5.09

0.67

CEthreader

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MRVDELRVDERIKSTLKER-GIESFYPPQAEALK-SGILEGKNALISIPTASGKTLIAEIAMVHRILTQGGKAVYIVPLKALAEEKFQEFQDWE-KIGLRVAMATGDYD--SKDEWLGKYDIIIATAEKFDSLLRHG--SSWIKDVKILVADEIHLIGSRDRGATLEVILAHML---GKAQIIGLSATIGNPEELAEWLNAELIVSDWRPVKLRRGVFYQGFVTWEDGSI--------------------DRFSSWEELVYDAIRKKGALIFVNMRRKAERVALELSKKVKSLL---TKPEIRALNELADSLEENPTNEKLAKAIRGGVAFHHAGLGRDERVLVEENFRKGIIKAVVATPTLSAGINTPAFRVIIRDIWRYSERIPIIEVHQMLGRAGRPKYDEVGEGIIVSTSDPREVMNHYIFGKPEKLFSQLSNES--NLRSQVLALIATFGYSTVEEILKFISNTFYAYQR---KDTYSLEEKIRNILYFLLENEFIEISLEDK--------IRPLSLGIRTAKLYIDPYTAKMFKDKMEEVVKDPNPIGIFHLISLTPDITPFNYSKREFERLEEEYYEFKDRL-------------YFDDPYISGYDPYLERKFFRAFKTALVLLAWINEVPEGEIVEKYSVEPGDIYRIVETAEWLVYSLKEIAKVLG-AYEIVDYLETLRVRVKYGIREELIPLMQLPLVGRRRARALYNSGFRSIEDISQARPEELLK-IEGIGVKTVEAIFKFLG-----------------------------------

5agaA

0.36

0.25

7.32

2.87

MUSTER

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKLLLANWGLPKAVLEKYHS-FGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGS---TSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFE----PMLQV-----KGDEDHVVSLCYETICNHSVLLFCPSKKWCEKLADIIAREFYNLHVILEQKELLEVMDQLRRLPSG-LDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQREVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASM----------GAIEACVMWLLENEFIQST-------EEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDW-TTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLG-WHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNEERRNMRTIWGRKGLTEREAAALIVEEARMILQ--QDL-----------

5agaA

0.35

0.24

7.15

2.63

HHsearch

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKLLLANWGLPKAVLEKYHS-FGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSP--S-RHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFE--PM-------LQVKGDEDHVVSLCYETIDNHSVLLFCPSKKWCEKLADIIAREFYNLHHILEQKELLEVMDQLRRL-PSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRGETGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASM----------GAIEACVMWLLENEFIQSTEE-------GKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFED-WTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLG-WHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNVPFKSARKETGRKGLTEREAAALIVEEARMILQQDL-------------

5agaA

0.35

0.23

6.97

5.17

FFAS-3D

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WGLPKAVLEKYHS-FGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRH---FSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLV--------REFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEQKELLEVMDQLRRLP-SGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQREVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMG----------AIEACVMWLLENEFIQSTEE-------GKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMF-EDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLG-WHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARVTGRKGLTEREAAALIVEEARMILQ--QDL-----------

5a9jA

0.36

0.25

7.44

4.27

CNFpred

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GRCLKAAAAGECKPTVPDYERDKLLL-----------------------ANWGLPKAVLEKYHSF-GVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGST---SPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYIT-AVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSS------MKLVREFEPMLQVKG---DEDHVVSLCYETICNHSVLLFCPSKKWCEKLADIIAREFYNLPVILEQKELLEVMDQLRRL-PSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQREVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMK------VQLGAIEACVMWLLENEFIQSTE-------EGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFED-WTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRL-GWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNA---RTIWVTGRKGLTEREAAALIVEEARMILQ----------------

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.7035

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4bgdA	0.81	3.99	0.115	0.889	model1_4bgdA.pdb.gz
2	4f91B	0.81	3.88	0.149	0.886	model1_4f91B.pdb.gz
3	5m59A	0.80	4.21	0.120	0.886	model1_5m59A.pdb.gz
4	5agaA	0.61	3.47	0.351	0.662	model1_5agaA.pdb.gz
5	2zjaA	0.60	2.48	0.262	0.631	model1_2zjaA.pdb.gz
6	2va8B	0.60	2.46	0.248	0.622	model1_2va8B.pdb.gz
7	2p6rA	0.58	2.90	0.260	0.613	model1_2p6rA.pdb.gz
8	5mc6h	0.52	4.19	0.133	0.575	model1_5mc6h.pdb.gz
9	4xgtA	0.52	4.10	0.162	0.570	model1_4xgtA.pdb.gz
10	4a4zA	0.52	3.99	0.172	0.570	model1_4a4zA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.97	catalytic activity
GO:0016787	0.94	hydrolase activity
GO:0017111	0.93	nucleoside-triphosphatase activity
GO:0004386	0.88	helicase activity
GO:0042623	0.82	ATPase activity, coupled
GO:0008026	0.77	ATP-dependent helicase activity
GO:1901363	0.76	heterocyclic compound binding
GO:0097159	0.76	organic cyclic compound binding
GO:0003676	0.59	nucleic acid binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.99	cellular process
GO:0071704	0.93	organic substance metabolic process
GO:0044260	0.92	cellular macromolecule metabolic process
GO:0044238	0.92	primary metabolic process
GO:0034641	0.92	cellular nitrogen compound metabolic process
GO:0090304	0.91	nucleic acid metabolic process
GO:0006259	0.65	DNA metabolic process
GO:0050896	0.56	response to stimulus
GO:0006950	0.52	response to stress
GO:0033554	0.51	cellular response to stress
GO:0006281	0.50	DNA repair

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0043229	0.90	intracellular organelle
GO:0044446	0.73	intracellular organelle part
GO:0044428	0.68	nuclear part
GO:0043231	0.68	intracellular membrane-bounded organelle
GO:0005634	0.66	nucleus
GO:0043232	0.59	intracellular non-membrane-bounded organelle
GO:0032991	0.54	macromolecular complex

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	3l9oA	0.480	4.26	0.161	0.537	3.6.4.13	NA
2	0.060	1hv8A	0.272	3.98	0.150	0.303	3.6.4.13	NA
3	0.060	1n1hA	0.288	9.43	0.040	0.457	2.7.7.48	NA
4	0.060	2pdaA	0.266	8.70	0.055	0.397	1.2.7.1	NA
5	0.060	2vdcF	0.276	9.72	0.028	0.444	1.4.1.13	364

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	3oypA	0.078	5.68	0.040	0.095	0.12	3OY	complex1.pdb.gz	319,405,406
2	0.01	1dxwA	0.079	5.96	0.054	0.099	0.18	2ZF	complex2.pdb.gz	320,321,326,366
3	0.01	1dy8A	0.078	6.24	0.053	0.099	0.11	III	complex3.pdb.gz	317,365,409,411
4	0.01	3keeA	0.077	5.47	0.019	0.094	0.10	30B	complex4.pdb.gz	320,325,364,413
5	0.01	1dxpA	0.086	6.10	0.083	0.108	0.12	III	complex5.pdb.gz	328,397,400,401
6	0.01	2vbcA	0.339	5.08	0.114	0.399	0.10	III	complex6.pdb.gz	385,468,469
7	0.01	3m5mB	0.089	6.11	0.053	0.113	0.11	III	complex7.pdb.gz	320,367,368,372,373
8	0.01	3oypB	0.079	6.07	0.067	0.100	0.12	III	complex8.pdb.gz	316,410,412
9	0.01	3lrrB	0.051	5.07	0.026	0.060	0.17	RQA	complex9.pdb.gz	326,367,368,405
10	0.01	3keeC	0.069	5.56	0.026	0.085	0.10	30B	complex10.pdb.gz	320,322,365,367

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.