D-I-TASSER Q8N5B7-D1

D-I-TASSER results for Q8N5B7-D1

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q8N5B7 (392 residues)
MATAAQGPLSLLWGWLWSERFWLPENVSWADLEGPADGYGYPRGRHILSVFPLAAGIFFV
RLLFERFIAKPCALCIGIEDSGPYQAQPNAILEKVFISITKYPDKKRLEGLSKQLDWNVR
KIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIRFLWSSPWFWDIRQCWHNYPF
QPLSSGLYHYYIMELAFYWSLMFSQFTDIKRKDFLIMFVHHLVTIGLISFSYINNMVRVG
TLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIFSAVFMVTRLGIYPFWILNTTLFES
WEIIGPYASWWLLNGLLLTLQLLHVIWSYLIARIALKALIRGKVSKDDRSDVESSSEEED
VTTCTKSPCDSSSSNGANRVNGHMGGSYWAEE

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MATAAQGPLSLLWGWLWSERFWLPENVSWADLEGPADGYGYPRGRHILSVFPLAAGIFFVRLLFERFIAKPCALCIGIEDSGPYQAQPNAILEKVFISITKYPDKKRLEGLSKQLDWNVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIRFLWSSPWFWDIRQCWHNYPFQPLSSGLYHYYIMELAFYWSLMFSQFTDIKRKDFLIMFVHHLVTIGLISFSYINNMVRVGTLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIFSAVFMVTRLGIYPFWILNTTLFESWEIIGPYASWWLLNGLLLTLQLLHVIWSYLIARIALKALIRGKVSKDDRSDVESSSEEEDVTTCTKSPCDSSSSNGANRVNGHMGGSYWAEE
Prediction	CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHSCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Confidence	98527789999999982677437999987773688998513678899999999999999999999985637999948747787899997225444331357885799999998855104478789999858765689999999878986899799999987417997778789970389888988999999999999999999999752123136776999999999999999612598899999875378999999999996730477799999999999999999989999987413312058602799999999999999999999999999999974997665546666643222233334565435555665566788887766679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MATAAQGPLSLLWGWLWSERFWLPENVSWADLEGPADGYGYPRGRHILSVFPLAAGIFFVRLLFERFIAKPCALCIGIEDSGPYQAQPNAILEKVFISITKYPDKKRLEGLSKQLDWNVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIRFLWSSPWFWDIRQCWHNYPFQPLSSGLYHYYIMELAFYWSLMFSQFTDIKRKDFLIMFVHHLVTIGLISFSYINNMVRVGTLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIFSAVFMVTRLGIYPFWILNTTLFESWEIIGPYASWWLLNGLLLTLQLLHVIWSYLIARIALKALIRGKVSKDDRSDVESSSEEEDVTTCTKSPCDSSSSNGANRVNGHMGGSYWAEE
Prediction	65352440042023000323000036030520554754231030200000011000000001011200020002101033533351642320020013234204321031003203103430121022223353423043004000100000000320010013110011032004402324033101000000000000000000142436512210000000010000001001100000000000100000100100210624400200000000102211310100000000000024101002001010130220021022013103200020024364460412465656656656556655555445434543434144441378
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHSCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MATAAQGPLSLLWGWLWSERFWLPENVSWADLEGPADGYGYPRGRHILSVFPLAAGIFFVRLLFERFIAKPCALCIGIEDSGPYQAQPNAILEKVFISITKYPDKKRLEGLSKQLDWNVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIRFLWSSPWFWDIRQCWHNYPFQPLSSGLYHYYIMELAFYWSLMFSQFTDIKRKDFLIMFVHHLVTIGLISFSYINNMVRVGTLIMCLHDVSDFLLEAAKLANYAKYQRLCDTLFVIFSAVFMVTRLGIYPFWILNTTLFESWEIIGPYASWWLLNGLLLTLQLLHVIWSYLIARIALKALIRGKVSKDDRSDVESSSEEEDVTTCTKSPCDSSSSNGANRVNGHMGGSYWAEE

5xtcr

0.11

0.09

3.28

1.31

CNFpred

-------PLLMLTTWLLPLTIMA----SQRHLSSEPLSRKKLYLSMLIFLQISLIMTFTATELIMFYIFETTLIPTLAIITRWGNQPERLNAGTYFLFYTLLPLLIALIYTHNTLSLNILLLTLTAQ---------ELSNSWANNLMWLAYTMAFMVKMPLYGLHLWLPKAHVEAP-----IAGSMVLAAVLLKLGGYGMMRLTLILNPLT-KHMAYPFLVLSLWGMIMTSSICLRQTDLKSLIAYSSISHMALVVTAILIQT-PWSFTGAVILMIAHGLTSSLLFCLANSNYERTHSRIMILSQGLQLPLMAFWWLLASLANLATINLLGELSVLVTTFSW--------------------------------------------------

2cqxA

0.64

0.12

3.35

1.19

HHsearch

---------------------------------------------------------------------GSSGSSGGIKDSPVNKVEPNDTLEKVFVSVTKYPDEKRLKGLSKQLDWSVRKIQCWFRHRRNQDKPSGPS----SG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

6f0kC

0.05

2.28

0.56

CEthreader

------------VLVQGGLTFHDITELVAQHTEKKTPKAWWAAFSVAFLGMLTLVAMLAYQVWNGVGVWGNNIPVGWGWPIVNFRQRWRTSINRAAEAMTIFAVICALIFPTFHVGRVWAIYWTLPIPNQMEMWPQFKSPLLWDVFAVSSYFIVSLVFWYVGLIPDLATLRDRAALMGRRLRYEKVYMLLAGLATPLVLSVHSVVSFDFAVSHTTIFPPYFVAGAIFSGFAMVVTHLEKMNIIMLVTGTMVGFAYITEFFIAWYSGVPYEQYAFINRATGP-YAWAYWTMMSCNLIFPQFFWIKKLRRNIPFMFIASIVVNIGMWFERFVITITSLHRDYLPSSWDYFVPTWVDVLTLIGSFGLFFTLFLLFLRFVPMVAIAEVKGVLPEAD

3j5pB

0.07

0.06

2.52

0.82

EigenThreader

KTCLLKAMLNLTIALLLDVARKTDSLKQFVNASYTDSYYKGQTALHIAIERRNMTLVTLLVEN-------------GADGRPGFYFGELPLSLAACT-----NQLAIVKFLLQNVGNTVLHALVEVALAASSGKIGVLAYILQFYFNFFVYCLYMIIFTAAAYYRPVEGL-------PPYKLKNTTGEILSVSGGVYFFFRGIQYFLQRRDSYSEILFFVQSLFMLVSVVLYFSQEYVASMVFSLAMGWTNMLYYTR------GFQQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLIEDGLLLAYVILTYLLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKAGGGGG----------------------

5z1fA

0.12

0.09

3.24

0.93

FFAS-3D

--VAAASAAQSLHC--QMVDKWTPRQLLWQNLNIK-----------LFSRIIRQYFIYFFVAVTILFYMIPIAFV---------SAITRTVLESFLPQ-------IALIVFL----AMLPKLLLFLSKAEGIPSQSHAIR--AASGKYFYFSVFNVFIGVTLAGT-----------LFNTDMIINLLATSLPKSATFFLTYVALKFFKTEAESYATRVPG-DMLILTITFCYSV-IAPLILIFGITYFGLGWLVLRNQALKVYVPSYESYGRMWPHIHQRILAALFLFQVVMFGYLGAK----------TFFYTALVIPLIITSLIFGYVCRQKFYGGFEHTALEVACRELKQSPDLEE---------------------------------

5gupl

0.09

3.26

0.88

SPARKS-K

MFIPVALFVTWSIMEFSMWYM-----------HSDPFINRFFKYLVLFLITMMILVTLFQLFIGWEGVGIMSFLLIGWWHGRT----DANTAALQAILYNRIGFVLSMAWFLTHSNWDLQQIFMLNNECPNMPLIGLLLAAAGKSWLPSAMEGPTPVSALLHSSTMVVAGVFLLIRFYPLMETNKLVQTMTLCLGAITTLFTALCAIT--QNDKKIVAFSTSSQLGLMMVTIGINQPHLAFLHICMHAKAMLFMCSGSIIHSLNDEPFTTTALIIGSLALTLT-----GFYSKDLIIEAVNMSYTNAW----ALLMTLIATSLTAAYSTRIIFFAFL--GKPRFPP----LVLINENNPLSNNIPPMTVPNTTMPLYMKMTALIVTIMGFML

4he8G

0.08

0.06

2.19

1.29

CNFpred

-------------------------------------------GLSALFFLTIALTVFLGALVARVRFLGLALLMEG-------------LLLGLFAAR-------DLLVFYVFFEAALIPALLMLYLYGG---EGRTRALYTFVLFTLVGSLPMLAAVLGARLLSPTFLLEDLLAH---LQEEAAFWVFLGFALAFAIKTPLFPLH----AWLPPFHQENHPSGLADALGTLYKVGVFAFFRFAIPLAPEGFAQAQGLLLFLA---ALSALYGAWVAFAAKDFKTLLAYAGLSHMGVAALGVFSGTPEGAMGGLYLLAASGVYTGGLFLLAGRLYERTG----------------------------------------------------

4iggA

0.05

0.04

1.79

0.83

DEthreader

DMDVYLLQLKVVEDGILKLYKPLYTASQACLQHPDVAAYANRDLIKQL-QA-------G-ALMADSSCTRDDRRERIVAECNAVQALDLLSEYMG--------------NAGRKERSD--ALNSAIDKMTKKTDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKN----------------VKEYAQVFREHANKLIEVANLAC-SISNN-EEGVKLVRMSASQLEALCQVNANMDLFKEQWEKQVRVTDVDTIDDFLAVSENHILEIRGRAARVIHVV-------------------------------------------TSEMDNYYTEKVLEADIRKAVLMI-T---------FEKKLEVSWSGNDILAGRMDRLAYLIAAVV-TV

5lq3A

0.06

0.05

2.30

0.82

MapAlign

-SVVAIIISLAGAIGLTNLPIEQYPSLTPPTVKVSKLGVTVRKTSSTTLAAISMYSAVDVYNYITLNVLDELKRVPGVGDASLRIWITATDVISAVNDQLQNPSEFENIILRNDGSFLR--LKDVADVEIGSQMINLQLLTLFALISPVISIVLV--LCAVFVQRQFALTLMISIINLPSASALHRTISEVDHISQEVQIIAELNKKKSYDEIQKDVNKLVTAVPFAVFGSFLLYFQTGLLLLIGLSAKNAIIVEFAMEERFKKGK-GVFEAAVAAAKLRFRPII-MTSLAFTFGVLPMIAGSASRHSLGTGLIGGMIAASLAIFFVPLFFYLLENFNEW----------------------------------------------------

3wajA1

0.10

0.09

3.52

0.61

MUSTER

YYRLIENTIHNFPHRIWDPFTYYPYGSYAGIIFSATSGESLRAVLAFIPAIGGVLAILPVYLLTREVFDKRAAVIAAF-QRSILGFNDHHIWEAFWQVS-NRWKGHDLTARQMAYPV-APIILAFMFFAFVLAGFVNADRKNLSLVAVVTFAVSALIYLPFAFNYPGFSTIFYSPF-QLLVLLGSAVIAAAFYQIEKWNDVGFFERVGLGRKGMPLAVIVLTALIMGLFFVLTNAVLHFG--ALFFFGMAGILYSAYRFLKRRSFPEMALLIWAIAMFIALQNRFAYYFAAVSAVYSALALSVVFDKLHFRVAFALLIALAAIYPTYILADAQS-----SYAGGPNKQWYDARENTPDGEKYDEYYLQLYPTPQSNKEPFSYPFETYGVISW

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5478

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3ayfA	0.45	6.12	0.055	0.681	model1_3ayfA.pdb.gz
2	6elhA	0.44	6.16	0.064	0.679	model1_6elhA.pdb.gz
3	6y7fA	0.44	3.85	0.087	0.541	model1_6y7fA.pdb.gz
4	6qq5A	0.44	5.86	0.047	0.650	model1_6qq5A.pdb.gz
5	2eabA	0.42	6.33	0.062	0.658	model1_2eabA.pdb.gz
6	4iggA	0.42	6.39	0.044	0.668	model1_4iggA.pdb.gz
7	4a01A	0.42	6.43	0.051	0.656	model1_4a01A.pdb.gz
8	3mk7A	0.33	6.55	0.046	0.520	model1_3mk7A.pdb.gz
9	2c2lA	0.26	6.24	0.063	0.398	model1_2c2lA.pdb.gz
10	2yevA1	0.24	3.86	0.047	0.298	model1_2yevA1.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0016410	1.00	N-acyltransferase activity
GO:0050291	0.98	sphingosine N-acyltransferase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0044699	1.00	single-organism process
GO:0071704	0.99	organic substance metabolic process
GO:0044763	0.99	single-organism cellular process
GO:0044710	0.99	single-organism metabolic process
GO:0044238	0.99	primary metabolic process
GO:0006629	0.98	lipid metabolic process
GO:1901576	0.97	organic substance biosynthetic process
GO:0044249	0.97	cellular biosynthetic process
GO:0034641	0.97	cellular nitrogen compound metabolic process
GO:0006665	0.97	sphingolipid metabolic process
GO:0046513	0.96	ceramide biosynthetic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005783	1.00	endoplasmic reticulum

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1ogyI	0.355	7.34	0.036	0.620	1.7.99.4	NA
2	0.060	2nyaA	0.360	7.11	0.043	0.610	1.7.99.4	NA
3	0.060	2p0mB	0.378	6.31	0.053	0.577	1.13.11.33	162
4	0.060	2dkiA	0.358	6.47	0.042	0.561	1.14.13.23	NA
5	0.060	2vuaA	0.229	7.21	0.042	0.406	3.4.24.69	191,230

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1syqA	0.277	4.72	0.060	0.360	0.45	III	complex1.pdb.gz	150,154,157,158,160,161,164,183,201,204,214,228,241
2	0.01	3rkoL	0.399	6.24	0.065	0.605	0.45	LFA	complex2.pdb.gz	158,161,162
3	0.01	1zw2A	0.267	5.01	0.078	0.360	0.43	III	complex3.pdb.gz	155,158,162,165,200,241,245,248,252,255,259
4	0.01	3tj5A	0.277	4.71	0.059	0.365	0.40	III	complex4.pdb.gz	157,158,161,165,196,199,241,244,248,252,255
5	0.01	2gwwA	0.277	4.49	0.071	0.357	0.46	III	complex5.pdb.gz	159,166,167,195,198,237,240,248,251

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.