D-I-TASSER Q8N394

D-I-TASSER results for Q8N394

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q8N394
Protein Name: Protein O-mannosyl-transferase TMTC2
Gene Name: TMTC2

Input Sequence in FASTA format

>Q8N394 (836 residues)
MIAELVSSALGLALYLNTLSADFCYDDSRAIKTNQDLLPETPWTHIFYNDFWGTLLTHSG
SHKSYRPLCTLSFRLNHAIGGLNPWSYHLVNVLLHAAVTGLFTSFSKILLGDGYWTFMAG
LMFASHPIHTEAVAGIVGRADVGASLFFLLSLLCYIKHCSTRGYSARTWGWFLGSGLCAG
CSMLWKEQGVTVLAVSAVYDVFVFHRLKIKQILPTIYKRKNLSLFLSISLLIFWGSSLLG
ARLYWMGNKPPSFSNSDNPAADSDSLLTRTLTFFYLPTKNLWLLLCPDTLSFDWSMDAVP
LLKTVCDWRNLHTVAFYTGLLLLAYYGLKSPSVDRECNGKTVTNGKQNANGHSCLSDVEY
QNSETKSSFASKVENGIKNDVSQRTQLPSTENIVVLSLSLLIIPFVPATNLFFYVGFVIA
ERVLYIPSMGFCLLITVGARALYVKVQKRFLKSLIFYATATLIVFYGLKTAIRNGDWQNE
EMLYRSGIKVNPAKAWGNLGNVLKSQSKISEAESAYRNALYYRSNMADMLYNLGLLLQEN
SRFAEALHYYKLAIGSRPTLASAYLNTGIILMNQGRTEEARRTFLKCSEIPDENLKDPHA
HKSSVTSCLYNLGKLYHEQGHYEEALSVYKEAIQKMPRQFAPQSLYNMMGEAYMRLSKLP
EAEHWYMESLRSKTDHIPAHLTYGKLLALTGRKSEAEKLFLKAIELDPTKGNCYMHYGQF
LLEEARLIEAAEMAKKAAELDSTEFDVVFNAAHMLRQASLNEAAEKYYDLAARLRPNYPA
ALMNLGAILHLNGRLQKAEANYLRALQLKPDDVITQSNLRKLWNIMEKQGLKTSKT

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q8N394-D1	1-332,387-479	425		MIAELVSSALGLALYLNTLSADFCYDDSRAIKTNQDLLPETPWTHIFYNDFWGTLLTHSGSHKSYRPLCTLSFRLNHAIGGLNPWSYHLVNVLLHAAVTGLFTSFSKILLGDGYWTFMAGLMFASHPIHTEAVAGIVGRADVGASLFFLLSLLCYIKHCSTRGYSARTWGWFLGSGLCAGCSMLWKEQGVTVLAVSAVYDVFVFHRLKIKQILPTIYKRKNLSLFLSISLLIFWGSSLLGARLYWMGNKPPSFSNSDNPAADSDSLLTRTLTFFYLPTKNLWLLLCPDTLSFDWSMDAVPLLKTVCDWRNLHTVAFYTGLLLLAYYGLKSPSLPSTENIVVLSLSLLIIPFVPATNLFFYVGFVIAERVLYIPSMGFCLLITVGARALYVKVQKRFLKSLIFYATATLIVFYGLKTAIRNGDWQN
2	Q8N394-D2	333-386	54		VDRECNGKTVTNGKQNANGHSCLSDVEYQNSETKSSFASKVENGIKNDVSQRTQ
3	Q8N394-D3	480-524	45		EEMLYRSGIKVNPAKAWGNLGNVLKSQSKISEAESAYRNALYYRS
4	Q8N394-D4	525-621	97		NMADMLYNLGLLLQENSRFAEALHYYKLAIGSRPTLASAYLNTGIILMNQGRTEEARRTFLKCSEIPDENLKDPHAHKSSVTSCLYNLGKLYHEQGH
5	Q8N394-D5	622-743	122		YEEALSVYKEAIQKMPRQFAPQSLYNMMGEAYMRLSKLPEAEHWYMESLRSKTDHIPAHLTYGKLLALTGRKSEAEKLFLKAIELDPTKGNCYMHYGQFLLEEARLIEAAEMAKKAAELDST
6	Q8N394-D6	744-836	93		EFDVVFNAAHMLRQASLNEAAEKYYDLAARLRPNYPAALMNLGAILHLNGRLQKAEANYLRALQLKPDDVITQSNLRKLWNIMEKQGLKTSKT

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.59

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6s7tA	0.45	5.40	0.083	0.553	model1_6s7tA.pdb.gz
2	3wajA	0.45	5.78	0.070	0.545	model1_3wajA.pdb.gz
3	7bvfA	0.43	5.98	0.090	0.543	model1_7bvfA.pdb.gz
4	6eznF	0.43	4.87	0.057	0.508	model1_6eznF.pdb.gz
5	6ftg5	0.42	4.99	0.102	0.505	model1_6ftg5.pdb.gz
6	7bvcA	0.42	6.37	0.057	0.550	model1_7bvcA.pdb.gz
7	7bvcB1	0.41	5.58	0.075	0.511	model1_7bvcB1.pdb.gz
8	5u1sA	0.41	7.20	0.067	0.575	model1_5u1sA.pdb.gz
9	7bveA1	0.41	5.83	0.084	0.517	model1_7bveA1.pdb.gz
10	5ezmA	0.41	4.82	0.089	0.487	model1_5ezmA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0003824	0.83	catalytic activity
GO:0016740	0.77	transferase activity
GO:0016757	0.74	transferase activity, transferring glycosyl groups
GO:0008375	0.71	acetylglucosaminyltransferase activity
GO:0016747	0.53	transferase activity, transferring acyl groups other than amino-acyl groups
GO:0008289	0.53	lipid binding
GO:1901981	0.52	phosphatidylinositol phosphate binding
GO:0098772	0.52	molecular function regulator
GO:0010485	0.52	H4 histone acetyltransferase activity
GO:0036094	0.51	small molecule binding
GO:0030234	0.51	enzyme regulator activity
GO:0033218	0.50	amide binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.77	cellular process
GO:0044699	0.74	single-organism process
GO:0065007	0.73	biological regulation
GO:0044763	0.63	single-organism cellular process
GO:0008152	0.61	metabolic process
GO:0071704	0.57	organic substance metabolic process
GO:0044238	0.55	primary metabolic process
GO:0044237	0.55	cellular metabolic process
GO:0043170	0.54	macromolecule metabolic process
GO:0044260	0.52	cellular macromolecule metabolic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0044424	0.97	intracellular part
GO:0044422	0.85	organelle part
GO:0044446	0.84	intracellular organelle part
GO:0043226	0.84	organelle
GO:0044444	0.80	cytoplasmic part
GO:0043229	0.79	intracellular organelle
GO:0043227	0.76	membrane-bounded organelle
GO:0043231	0.75	intracellular membrane-bounded organelle
GO:0016020	0.74	membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vkzG	0.320	8.25	0.039	0.495	2.3.1.38 3.1.2.14	NA
2	0.060	3ebgA	0.272	7.86	0.052	0.404	3.4.11.-	NA
3	0.060	3b9jC	0.205	8.18	0.027	0.313	1.17.3.2 1.17.1.4	NA
4	0.060	1ub2A	0.275	8.42	0.043	0.433	1.11.1.7	NA
5	0.060	1w6jA	0.309	7.33	0.048	0.444	5.4.99.7	117,120

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1jppA	0.310	6.20	0.066	0.411	0.25	III	complex1.pdb.gz	481,485,519
2	0.01	3p5rB	0.317	7.52	0.054	0.464	0.20	FGG	complex2.pdb.gz	497,500,501,504,562
3	0.01	1jpwC	0.308	6.19	0.051	0.409	0.18	III	complex3.pdb.gz	497,501,566,568,569
4	0.01	2gl7A	0.317	6.09	0.046	0.416	0.21	III	complex4.pdb.gz	480,483,486,490,498
5	0.01	1t08A	0.311	6.30	0.059	0.416	0.25	III	complex5.pdb.gz	501,502,505,574,607,613,614
6	0.01	1jppB	0.298	6.14	0.050	0.394	0.20	III	complex6.pdb.gz	494,497,501,531,535,538,539
7	0.01	3p5rA	0.302	7.90	0.044	0.453	0.18	FGG	complex7.pdb.gz	486,498,501,502,505
8	0.01	2ot8A	0.344	6.99	0.046	0.472	0.25	III	complex8.pdb.gz	494,497,498,501,532
9	0.01	1i7x0	0.311	6.13	0.064	0.411	0.39	III	complex9.pdb.gz	492,498,523,526,529
10	0.01	1h39B	0.305	6.47	0.036	0.406	0.10	R03	complex10.pdb.gz	501,529,531,532

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.