D-I-TASSER Q8N0Z9

D-I-TASSER results for Q8N0Z9

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q8N0Z9
Protein Name: V-set and immunoglobulin domain-containing protein 10
Gene Name: VSIG10

Input Sequence in FASTA format

>Q8N0Z9 (540 residues)
MAAGGSAPEPRVLVCLGALLAGWVAVGLEAVVIGEVHENVTLHCGNISGLRGQVTWYRNN
SEPVFLLSSNSSLRPAEPRFSLVDATSLHIESLSLGDEGIYTCQEILNVTQWFQVWLQVA
SGPYQIEVHIVATGTLPNGTLYAARGSQVDFSCNSSSRPPPVVEWWFQALNSSSESFGHN
LTVNFFSLLLISPNLQGNYTCLALNQLSKRHRKVTTELLVYYPPPSAPQCWAQMASGSFM
LQLTCRWDGGYPDPDFLWIEEPGGVIVGKSKLGVEMLSESQLSDGKKFKCVTSHIVGPES
GASCMVQIRGPSLLSEPMKTCFTGGNVTLTCQVSGAYPPAKILWLRNLTQPEVIIQPSSR
HLITQDGQNSTLTIHNCSQDLDEGYYICRADSPVGVREMEIWLSVKEPLNIGGIVGTIVS
LLLLGLAIISGLLLHYSPVFCWKVGNTSRGQNMDDVMVLVDSEEEEEEEEEEEEDAAVGE
QEGAREREELPKEIPKQDHIHRVTALVNGNIEQMGNGFQDLQDDSSEEQSDIVQEEDRPV

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q8N0Z9-D1	1-121	121		MAAGGSAPEPRVLVCLGALLAGWVAVGLEAVVIGEVHENVTLHCGNISGLRGQVTWYRNNSEPVFLLSSNSSLRPAEPRFSLVDATSLHIESLSLGDEGIYTCQEILNVTQWFQVWLQVAS
2	Q8N0Z9-D2	122-221	100		GPYQIEVHIVATGTLPNGTLYAARGSQVDFSCNSSSRPPPVVEWWFQALNSSSESFGHNLTVNFFSLLLISPNLQGNYTCLALNQLSKRHRKVTTELLVY
3	Q8N0Z9-D3	222-309	88		YPPPSAPQCWAQMASGSFMLQLTCRWDGGYPDPDFLWIEEPGGVIVGKSKLGVEMLSESQLSDGKKFKCVTSHIVGPESGASCMVQIR
4	Q8N0Z9-D4	310-406	97		GPSLLSEPMKTCFTGGNVTLTCQVSGAYPPAKILWLRNLTQPEVIIQPSSRHLITQDGQNSTLTIHNCSQDLDEGYYICRADSPVGVREMEIWLSVK
5	Q8N0Z9-D5	407-540	134		EPLNIGGIVGTIVSLLLLGLAIISGLLLHYSPVFCWKVGNTSRGQNMDDVMVLVDSEEEEEEEEEEEEDAAVGEQEGAREREELPKEIPKQDHIHRVTALVNGNIEQMGNGFQDLQDDSSEEQSDIVQEEDRPV

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.58

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1e07A	0.47	6.42	0.087	0.678	model1_1e07A.pdb.gz
2	3b43A	0.47	5.96	0.094	0.656	model1_3b43A.pdb.gz
3	6vg1A	0.46	6.39	0.059	0.680	model1_6vg1A.pdb.gz
4	5t9tA	0.46	6.22	0.055	0.663	model1_5t9tA.pdb.gz
5	4ofyD	0.46	5.91	0.144	0.637	model1_4ofyD.pdb.gz
6	6e6bA	0.46	6.25	0.056	0.665	model1_6e6bA.pdb.gz
7	6vg4A	0.45	6.00	0.047	0.637	model1_6vg4A.pdb.gz
8	5dzvA	0.45	6.27	0.054	0.659	model1_5dzvA.pdb.gz
9	5sznA	0.45	6.08	0.053	0.637	model1_5sznA.pdb.gz
10	5lf5A	0.45	5.70	0.080	0.609	model1_5lf5A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0042802	0.55	identical protein binding
GO:0005198	0.55	structural molecule activity
GO:0003723	0.52	RNA binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	1.00	regulation of cellular process
GO:0048518	1.00	positive regulation of biological process
GO:0044699	0.76	single-organism process
GO:0009987	0.72	cellular process
GO:0044763	0.69	single-organism cellular process
GO:0048522	0.63	positive regulation of cellular process
GO:0048583	0.60	regulation of response to stimulus
GO:0032502	0.59	developmental process
GO:0019222	0.59	regulation of metabolic process
GO:0007165	0.59	signal transduction
GO:0080090	0.58	regulation of primary metabolic process
GO:0060255	0.58	regulation of macromolecule metabolic process
GO:0048584	0.58	positive regulation of response to stimulus
GO:0031323	0.58	regulation of cellular metabolic process
GO:0032270	0.57	positive regulation of cellular protein metabolic process
GO:0002376	0.57	immune system process
GO:0010468	0.56	regulation of gene expression
GO:2000112	0.55	regulation of cellular macromolecule biosynthetic process
GO:0051171	0.55	regulation of nitrogen compound metabolic process
GO:0010628	0.55	positive regulation of gene expression
GO:0044767	0.54	single-organism developmental process
GO:0002682	0.54	regulation of immune system process
GO:0050776	0.53	regulation of immune response
GO:0045727	0.52	positive regulation of translation
GO:0002764	0.52	immune response-regulating signaling pathway
GO:0080134	0.50	regulation of response to stress
GO:0050778	0.50	positive regulation of immune response

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044464	1.00	cell part
GO:0016021	1.00	integral component of membrane
GO:0016020	1.00	membrane
GO:0005886	0.91	plasma membrane
GO:0043226	0.81	organelle
GO:0043227	0.79	membrane-bounded organelle
GO:0044459	0.77	plasma membrane part
GO:0044424	0.76	intracellular part
GO:0043229	0.66	intracellular organelle
GO:0043231	0.63	intracellular membrane-bounded organelle
GO:0031226	0.62	intrinsic component of plasma membrane
GO:0005887	0.61	integral component of plasma membrane
GO:0044421	0.57	extracellular region part
GO:0005634	0.54	nucleus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vkzG	0.282	7.84	0.062	0.474	2.3.1.38 3.1.2.14	NA
2	0.060	2hr7A	0.188	7.95	0.027	0.324	2.7.10.1	26
3	0.060	3mjgX	0.277	3.71	0.103	0.326	2.7.10.1	NA
4	0.060	2np0A	0.230	7.50	0.035	0.372	3.4.24.69	NA
5	0.060	1kv3A	0.211	7.08	0.042	0.326	2.3.2.13	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	2vraB	0.313	2.46	0.158	0.337	0.43	UUU	complex1.pdb.gz	50,51,52,53,110
2	0.01	3gbn1	0.166	1.93	0.168	0.174	0.53	III	complex2.pdb.gz	53,55,57,59,62,63,65,68,102,105
3	0.01	3mlwM	0.166	2.99	0.097	0.181	0.55	III	complex3.pdb.gz	23,52,53,106

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.