D-I-TASSER Q8IYB8-D3

D-I-TASSER results for Q8IYB8-D3

[Click result.zip to download all results on this page]

Input Sequence in FASTA format

>Q8IYB8 (613 residues)
DCKDDLRKISDLRIPPNWYPDARAMQRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPL
KLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEMCSVTTPYEVAVIDEI
QMIRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVMELMYTTGEEVEVRDYKRLTPISV
LDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFND
PNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGERELEPITTSQALQIAGRAGR
FSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLI
DIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQF
ARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDAS
LIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGT
KALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHP
KGTRRKKKEPDSD

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| DCKDDLRKISDLRIPPNWYPDARAMQRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEMCSVTTPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVMELMYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD
Prediction	CHHHHHHHHHCCCCCHHHHHHHHHHCCCSSSSSCCCCCCHHHHHHHHHHHCCCSSSSHHHHHHHHHHHHHHHHHCCCSSSSSCCSSSSCCCCCCCCCSSSSCHHHHCCCCCCCSSSSSSSSSCCCCCCCHHHHHHHHHCCCCSSSSSSCCCCHHHHHHHHHHHCCSSSSSSSCCCCCSSSSSSCCHHHCCCCCCCSSCHHHHHHHHHHHHHHHHCCCCSSSSCCCCCHHHHHHHHHHHHHCCCCCCSSSSSHHHHCCCCCCCSSSSSSCCCCCCCCCCCCCCCCSCCHHHHHHHHHHCCCCCCCCCCSSSSSSCHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Confidence	8478999985568955660888861598499987999857999999999679689960499999999999997198445872744653344479998999842332178995489996002136733247999999727864499983863169999999975993999970589972998530200103455420111159999999999996098355450899988999999997523798008997323433899874499993344456655444444118999999998741777778874599991846799999998099975033057896899999999748875999999999861246422344899999999998526888999998852998656689999999999999938857848888631345667777899999999999999999999864556899999999999999999999999999999888888877652131146676442133322344443322123466654322102799999863399899999999999743444433444455666677777898899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| DCKDDLRKISDLRIPPNWYPDARAMQRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEMCSVTTPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVMELMYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD
Prediction	7336304602603414411240220543000000000010000001102434100000001000340053037321300000032223245535501000000000111220000000000000053001000200230222100000012014002200410613020232610030211230131142133222012101200130021022241300100010246203300420432632020000010012303020100001104123344333342320204202210110223345232000000255005103400735343043020200110010003104643033004202511514320101306303400510562613130002002000435252004002300310175440415203420523433542342043003103000000000110032022361035005302520350044035234444444336355453535543454255444446535344453445444534434004301640222461055035304554465445445554455555455554778
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Contact, Hydrogen and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

Hydrogen bond networks

D-I-TASSER simulation is guided by the consensus contact map (left figure), distance map (middle figure) and Hydrogen bond network (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Top 10 threading templates used by D-I-TASSER

Rank

PDB
hit

ID1

ID2

Cov

Norm.
Zscore

Download
alignment

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

SS
Seq

CHHHHHHHHHCCCCCHHHHHHHHHHCCCSSSSSCCCCCCHHHHHHHHHHHCCCSSSSHHHHHHHHHHHHHHHHHCCCSSSSSCCSSSSCCCCCCCCCSSSSCHHHHCCCCCCCSSSSSSSSSCCCCCCCHHHHHHHHHCCCCSSSSSSCCCCHHHHHHHHHHHCCSSSSSSSCCCCCSSSSSSCCHHHCCCCCCCSSCHHHHHHHHHHHHHHHHCCCCSSSSCCCCCHHHHHHHHHHHHHCCCCCCSSSSSHHHHCCCCCCCSSSSSSCCCCCCCCCCCCCCCCSCCHHHHHHHHHHCCCCCCCCCCSSSSSSCHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DCKDDLRKISDLRIPPNWYPDARAMQRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEMCSVTTPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVMELMYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD

3rc3A

0.92

0.74

20.71

1.17

DEthreader

DCKDDLRKISDLRIPPNWYPDARA-QRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAA-GVPCDLVTEERVTVQPNGKQASHVSCTVE-CSVTTPYEVAVIDEIQ-IRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVEL-YT-TGEEVEVRDYKRLPISVLDHALESLDNLR-PGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIG-GLNLSIRRIIFYSLIK-----------EPITTSQALQIAGRAGRFSSRFKEGEVTT-NHEDLSLLKEILKRPVDPIRAAGLHPTAEQIE-FAYHLPDATLSNLIDIFVDFSQVDGQYFVCND-DFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDL-DLEAVHDVLDLYLWLSYRF-D-FPDASLIRDLQKELDGIIQDGVHNITKLIKSE-----------------------------------------------THKLL-N-LE-------------------------------------------------

6zm2A

0.15

0.14

4.66

1.71

SPARKS-K

---TIQEVRKSL--PVYRDAFLDAVKEQVLILVGETGSGKTTQIPQYLHEAGYTACTQPRRVAAMSVAARVADEMVRLGHEVGYSIRFEDT-SEKTILKYMTDGMLPDLADYSCIMIDEAHERT------VHTDILLALIRPELRLIISSATLN-AEKFSAYFDHPVEVYYTPESNYLEAALVTVFQIHATQPGDILVFLTQEEIERACERVEEIRIIALPIYSNMPSEMQAKIFE--PTPPGARKVVFSTNIAETSLTIGIVYVIDSGVKENTFSPVGTLAVVPCSRAAANQRMGRAGRVKP----GKCFRLYTK-YAYLSEMDESPTPEIQRTSLSSVVLQLKAFLDPPPTELLIKSLNMLYALGALNSAGQL------TRVGRQMGEFPTEPMLAKALIAATQEGCVSEVLTIVSMLVGTLFFRPKDKKVHADSARARFTVRD--------GGDHLTLLNIYNQWVEAEY----SPIWARELAQRSLTRARDVRDQLAKLCDRIL-----------DGSEASCGGVNNPTPILRALTAAFFLNAARLNRAGDGYRTLKNNITVYVHPSSVVRGMDPPPKVIIYHELVVTSKEYVRSVIPVEPRWLSE---

3rc3A

0.97

0.78

21.77

2.42

SPARKS-K

DCKDDLRKISDLRIPPNWYPDARA-QRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEC-SVTTPYEVAVIDEIQI-RDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVE--LYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIG-GLNLSIRRIIFYSL-----------IKEPITTSQALQIAGRAGRFSSRFKEGEVTT-NHEDLSLLKEILKRPVDPIRAAGLHPTAEQIE-FAYHLPDATLSNLIDIFVDFSQVDGQYFVCN-DDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDL-DLEAVHDVLDLYLWLSYRFD--FPDASLIRDLQKELDGIIQDGVHNITKLIKSETHKLLNLE--------------------------------------------------------------------------------------------------

6fa5A

0.15

0.14

4.63

1.65

SPARKS-K

AQRTIQEVRKSLPVYAYRDAFLDAVKEQVLILVGETGSGKTTQIPQYLHEARKIACTQPRRVAAMSVAARVADEGVRLGHEVGYSIRFEDCTSEKTILKYMTDGMLPDLADYSCIMIDEAHERT------VHTDILLALIRPELRLIISSATLN-AEKFSAYFDHPVEVYYTSAPES-NYLEAALVTVFQIPEGDILVFLTQEEIERACERVEEIRIIALPIYSNMPSEMQAKIFE--PTPPGARKVVFSTNIAETSLTIDIVYVIDSGYVKENTFSPVGLAVVPCSRAAANQRMGRAGRVKP----GKCFRLYTK-YAYLSEMDESPTPEIQRTSLSSVVLQLKAFLDPPPTELLIKSLNMLYALGALNSAGQL------TRVGRQMGEFPTEPMLAKALIAATQEGCVSEVLTIVSMLVGTLFFRPKDKKVHADSARARFTVRD--------GGDHLTLLNIYNQWVEAE----YSPIWARELAQRSLTRARDVRDQLAKLCDRIL--------DGSEASCGGVNNPTPILRALTAAFFLNAARLNRAGDGYRTLKNNITVYVHPSSVVRGMDPPPKVIIYHELVVTSKEYVRSVIPVEPRWLSEFG----

3rc3A

0.95

0.76

21.28

1.18

MapAlign

-CKDDLRKISDLRIPPNWYPDAR-AQRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVE-CSVTTPYEVAVIDEIQ-IRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLV--ELYTTGEEVEVRDYKRLTPISVLDHALESLDNLR-PGDCIVCFKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIG-GLNLSIRRIIFYSLIK-----------EPITTSQALQIAGRAGRFSSRFKEGEVTTNH-EDLSLLKEILKRPVDPIRAAGLHPTAEQIE-FAYHLPDATLSNLIDIFVDFSQVDGQYFVCND-DFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDL-DLEAVHDVLDLYLWLSYRF--DFPDASLIRDLQKELDGIIQDGVHNITKLI---------------------------------------------------------KSETHKLLNLE-----------------------------------------

6h57A

0.16

0.15

5.04

1.29

HHsearch

RSDEIQKARIQL--PVFGEEHMEAIHNDVVIICGETGSGKTTQVPQFLYEAGPVGITQPRRVAAVSMAERVAELG-DHGHKVGYQIRFDSTAKEDTKVKFMTDGVLFKLTKYSSIIIDEAHE-----RNIN-TDILIGMLRKKLKLIIMSATLRV-SDFSENFARPVSIHFNR-RTAFNYTDEAFRKTCKLPPGAILVFLTQQEITHMVKRLRKEPLYVLPLYSLLPTK---EQMRVFQKPQGSRLCIVATNVAETSLTIPVRYVVDSGRSKERYNESNSFEVGWVSKASANQRSGRAGRTGP----GHCYRLYSSA--VFEHFEQFSKPEILRMPVESIVLQMKSMPFPTPPVALSKAIQLLQYLGALDN------KEMITEDGKKMSLFPLSPRFSKMLLVSDEKACLPYIVAIVSALSVGFINEFELGSKFYKSRSQFSK--LDKFSDVFRLLSVVSAMDKEIFMKKNFLR-GKLMEEIVKLRKQLMYIIKSNTSAVVKLLKQMICAGFVDHVAVRADVLITNIPYIPEDCVYHPTSILNNYMLYYSLHL-GGNNK--TRMNTLCDIASTPLANIARKGLLYSKPLTGQGLKTVNLSPTERYCYVVSDKI

3rc3A

0.97

0.78

21.90

0.90

CEthreader

DCKDDLRKISDLRIPPNWYPDARA-QRKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVE-CSVTTPYEVAVIDEIQ-IRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVE--LYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIG-GLNLSIRRIIFYSLIK-----------EPITTSQALQIAGRAGRFSSRFKEGEVTT-NHEDLSLLKEILKRPVDPIRAAGLHPTAEQIE-FAYHLPDATLSNLIDIFVDFSQVDGQYFVCND-DFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDL-DLEAVHDVLDLYLWLSYRFD--FPDASLIRDLQKELDGIIQDGVHNITKLIKSETHKLLNLE--------------------------------------------------------------------------------------------------

5agaA

0.17

0.15

4.87

2.41

FFAS-3D

AVLEKYHS-FGVKKMFEWQAECLLLEGKNLVYSAPTSAGKTLLILKRVLEMRKKLFILPFVSVAKEKKYYLQSLGIKVDGYMGST--SPSRHFSSLDIAVCTIERANKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICNAVQIVGMSATLPNL--ELVASWLNAELYHTDFRPVPLLEVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYGVAFHHAGLTFEERDIIEGAFR--QGLIRVLAATSTLSSGVNLPARRVIIRTPI---------FGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRVGGVASTSQDMHTYAACTSSLSPADTLDIFADLQRAMKGFVLENDLDWYRFFCLWEKLPTSMKRVAELVGVERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGC------NRGQIQSLQSAAVYAGMITVFSNRLG-WHNMELLLSQFQKRLTFGIQRELCDLVRV--------------------------------------------------SLLNAQRARVLYASGFHTVADLVEVEVILKNAVPFKSARKEAVEE-----RRNMRT----

3rc3A

0.97

0.77

21.68

1.75

MUSTER

DCKDDLRKISDLRIPPNWYPDARA--RKIIFHSGPTNSGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEC-SVTTPYEVAVIDEIQ-IRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVE--LYTTGEEVEVRDYKRLTPISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKILVATDAIG-GLNLSIRRIIFYSLIK-----------EPITTSQALQIAGRAGRFSSRFKEGEVTT-NHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEFAYH-LPDATLSNLIDIFVDFSQVDGQYFVCN-DDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDL-DLEAVHDVLDLYLWLSYRFD--FPDASLIRDLQKELDGIIQDGVHNITKLIKSETHKLLNLE--------------------------------------------------------------------------------------------------

6o16A

0.13

0.12

4.27

1.55

SPARKS-K

EMQEERLKL-----PIEEQAIMEAVAEPIVIVCGETGSGKTTQVPQFLYEAGYSGVTEPRRVAAVAMSQRVAKENLSHRV-VSYQIRYEGNVTEETRIKFMTDGVLFLLLKYKVVIIDEAHERS------VYTDILLGLLSLPLKLLIMSATLR-VEDFTQNQRPVIKVVHFNDDYSGECFRKVCKIHRMLPAGGILVFLTQAEVHALCRRLRKAFLHVLPLYSLLAP---EKQAQVFKPPPGTRLCVVATNVAETSLTIPIKYVVDCGKVKKRYDRVTGFRVTWVSQASADQRAGRAGRTEP----GHCYRLYSS--AVFGDFEQFPPPEITRRPVEDLILQMKAFPTPPSVEALVAAEELLVALGALQASCP------ITALGRTMSTFPVAPRYAKMLALSQQHGCLPYTIAIVAAMTKELAELKGRRARVAQMKRTWAGQ-----GPSLKLGDLMVLLGAVGACEYAG----CSPQFCQALRYKAMLEIRRLRGQLTTAVNAVC-----------PEDPKMQPPTESQVTYLRQIMAAGLGDHLARRVQSLDPKWKNAYKTPLLDDPVFIHPSSVLFKELPEFVVYQEIVETTKMYMKGVSTVEIQWIP

(a)	ID1 is the number of template residues identical to query divided by number of aligned residues.
(b)	ID2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment and is highlighted in bold.
(e)	Download alignment lists the threading program used to identify the template, and provide the 3D structure of aligned regions of threading templates (threading[1-10].pdb.gz).
(f)	Template residues identical to query sequence are highlighted in color.

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.6068

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	3rc3A	0.79	1.05	0.978	0.803	model1_3rc3A.pdb.gz
2	6f4aB	0.67	1.70	0.349	0.693	model1_6f4aB.pdb.gz
3	5aorA	0.62	5.29	0.114	0.783	model1_5aorA.pdb.gz
4	5n8rA	0.61	5.37	0.096	0.776	model1_5n8rA.pdb.gz
5	5vhaA	0.60	4.84	0.112	0.733	model1_5vhaA.pdb.gz
6	6zm2A	0.59	5.23	0.114	0.754	model1_6zm2A.pdb.gz
7	6c90A	0.59	5.07	0.163	0.715	model1_6c90A.pdb.gz
8	4xgtA	0.59	4.78	0.164	0.706	model1_4xgtA.pdb.gz
9	5mc6h	0.59	4.57	0.178	0.697	model1_5mc6h.pdb.gz
10	6o16A	0.59	5.36	0.119	0.759	model1_6o16A.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0004386	1.00	helicase activity
GO:1901363	0.90	heterocyclic compound binding
GO:0097159	0.90	organic cyclic compound binding
GO:0003678	0.85	DNA helicase activity
GO:0003676	0.85	nucleic acid binding
GO:0003677	0.78	DNA binding
GO:0016887	0.74	ATPase activity
GO:0003724	0.72	RNA helicase activity
GO:0008026	0.66	ATP-dependent helicase activity
GO:0003723	0.65	RNA binding
GO:0004004	0.62	ATP-dependent RNA helicase activity
GO:0034458	0.50	3'-5' RNA helicase activity
GO:0003725	0.50	double-stranded RNA binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.96	cellular process
GO:0008152	0.89	metabolic process
GO:0044237	0.87	cellular metabolic process
GO:0071704	0.85	organic substance metabolic process
GO:0044238	0.85	primary metabolic process
GO:0044260	0.84	cellular macromolecule metabolic process
GO:0034641	0.84	cellular nitrogen compound metabolic process
GO:0090304	0.83	nucleic acid metabolic process
GO:0016070	0.81	RNA metabolic process
GO:0016043	0.77	cellular component organization
GO:0006996	0.76	organelle organization
GO:0065007	0.73	biological regulation
GO:0050789	0.72	regulation of biological process
GO:0032508	0.72	DNA duplex unwinding
GO:0044699	0.70	single-organism process
GO:0050794	0.68	regulation of cellular process
GO:0006396	0.66	RNA processing
GO:0048522	0.64	positive regulation of cellular process
GO:0016071	0.64	mRNA metabolic process
GO:0006401	0.64	RNA catabolic process
GO:0044763	0.63	single-organism cellular process
GO:0000959	0.63	mitochondrial RNA metabolic process
GO:0060255	0.60	regulation of macromolecule metabolic process
GO:0080090	0.59	regulation of primary metabolic process
GO:0051171	0.59	regulation of nitrogen compound metabolic process
GO:0031323	0.59	regulation of cellular metabolic process
GO:0000965	0.59	mitochondrial RNA 3'-end processing
GO:0006402	0.57	mRNA catabolic process
GO:0051173	0.56	positive regulation of nitrogen compound metabolic process
GO:0044767	0.56	single-organism developmental process
GO:0031325	0.56	positive regulation of cellular metabolic process
GO:0010604	0.56	positive regulation of macromolecule metabolic process
GO:0051128	0.55	regulation of cellular component organization
GO:0006259	0.55	DNA metabolic process
GO:0048869	0.53	cellular developmental process
GO:0040008	0.52	regulation of growth
GO:0000957	0.52	mitochondrial RNA catabolic process
GO:0051252	0.51	regulation of RNA metabolic process
GO:0048519	0.51	negative regulation of biological process
GO:0006310	0.51	DNA recombination
GO:0048523	0.50	negative regulation of cellular process
GO:0043933	0.50	macromolecular complex subunit organization

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0044444	0.97	cytoplasmic part
GO:0044446	0.94	intracellular organelle part
GO:0044429	0.91	mitochondrial part
GO:0043226	0.87	organelle
GO:0043229	0.86	intracellular organelle
GO:0005759	0.86	mitochondrial matrix
GO:0043231	0.81	intracellular membrane-bounded organelle
GO:0005739	0.73	mitochondrion
GO:0032991	0.71	macromolecular complex
GO:0043234	0.68	protein complex
GO:0005634	0.65	nucleus
GO:0000178	0.63	exosome (RNase complex)
GO:0098798	0.62	mitochondrial protein complex
GO:0000177	0.61	cytoplasmic exosome (RNase complex)
GO:0045025	0.60	mitochondrial degradosome

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2vdcA	0.345	8.36	0.049	0.594	1.4.1.13	NA
2	0.060	1a1qA	0.121	5.82	0.040	0.166	3.4.21.98	NA
3	0.060	3l9oA	0.550	4.88	0.149	0.669	3.6.4.13	36,40,63,119,258,296,299
4	0.060	1ofdA	0.346	8.32	0.049	0.589	1.4.7.1	NA
5	0.060	3pjrA	0.350	5.40	0.091	0.449	3.6.4.12	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.74	3rc3A	0.811	1.04	0.986	0.822	1.92	ANP	complex1.pdb.gz	36,37,38,39,40,41,42,175,256,293,297,300,301,302
2	0.15	3rc8A	0.804	1.55	1.000	0.824	1.00	QNA	complex2.pdb.gz	60,61,83,102,104,105,128,224,251,252,253,272
3	0.02	2jlyA	0.470	5.03	0.117	0.578	0.98	PO4	complex3.pdb.gz	34,35,40,119,149,293,297

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.