D-I-TASSER Q8IY95

D-I-TASSER results for Q8IY95

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Summary of the Protein

Uniprot ID: Q8IY95
Protein Name: Transmembrane protein 192
Gene Name: TMEM192

Input Sequence in FASTA format

>Q8IY95 (271 residues)
MAAGGRMEDGSLDITQSIEDDPLLDAQLLPHHSLQAHFRPRFHPLPTVIIVNLLWFIHLV
FVVLAFLTGVLCSYPNPNEDKCPGNYTNPLKVQTVIILGKVILWILHLLLECYIQYHHSK
IRNRGYNLIYRSTRHLKRLALMIQSSGNTVLLLILCMQHSFPEPGRLYLDLILAILALEL
ICSLICLLIYTVKIRRFNKAKPEPDILEEEKIYAYPSNITSETGFRTISSLEEIVEKQGD
TIEYLKRHNALLSKRLLALTSSDLGCQPSRT

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q8IY95-D1	1-271	271		MAAGGRMEDGSLDITQSIEDDPLLDAQLLPHHSLQAHFRPRFHPLPTVIIVNLLWFIHLVFVVLAFLTGVLCSYPNPNEDKCPGNYTNPLKVQTVIILGKVILWILHLLLECYIQYHHSKIRNRGYNLIYRSTRHLKRLALMIQSSGNTVLLLILCMQHSFPEPGRLYLDLILAILALELICSLICLLIYTVKIRRFNKAKPEPDILEEEKIYAYPSNITSETGFRTISSLEEIVEKQGDTIEYLKRHNALLSKRLLALTSSDLGCQPSRT

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.546

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	1w27A	0.57	5.23	0.053	0.856	model1_1w27A.pdb.gz
2	6at7B	0.57	5.41	0.057	0.841	model1_6at7B.pdb.gz
3	3nz4A	0.56	5.29	0.072	0.838	model1_3nz4A.pdb.gz
4	6elhA	0.56	4.83	0.075	0.771	model1_6elhA.pdb.gz
5	2yevA	0.55	4.74	0.037	0.779	model1_2yevA.pdb.gz
6	7jroa	0.55	4.73	0.101	0.775	model1_7jroa.pdb.gz
7	1ocrN	0.55	4.74	0.037	0.779	model1_1ocrN.pdb.gz
8	1y2mB	0.55	5.49	0.069	0.849	model1_1y2mB.pdb.gz
9	3mk7A	0.54	4.52	0.058	0.727	model1_3mk7A.pdb.gz
10	3o0rB	0.53	4.62	0.043	0.731	model1_3o0rB.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0042803	1.00	protein homodimerization activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0009987	0.81	cellular process
GO:0044699	0.71	single-organism process
GO:0044763	0.68	single-organism cellular process
GO:0006810	0.61	transport
GO:0044765	0.59	single-organism transport
GO:0055085	0.54	transmembrane transport
GO:0006812	0.54	cation transport
GO:1902600	0.53	hydrogen ion transmembrane transport

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0070062	1.00	extracellular exosome
GO:0048471	1.00	perinuclear region of cytoplasm
GO:0005770	1.00	late endosome
GO:0005765	1.00	lysosomal membrane
GO:0005764	1.00	lysosome
GO:0005654	1.00	nucleoplasm
GO:0005886	0.64	plasma membrane
GO:0005634	0.58	nucleus
GO:0005794	0.56	Golgi apparatus
GO:0005783	0.56	endoplasmic reticulum

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1yisA	0.468	5.08	0.048	0.697	4.3.2.2	246
2	0.060	2occN	0.553	4.73	0.037	0.779	1.9.3.1	NA
3	0.060	1k62B	0.468	4.80	0.064	0.675	4.3.2.1	NA
4	0.060	2e9eA	0.442	6.12	0.033	0.749	1.11.1.7	NA
5	0.060	1jswB	0.472	4.66	0.047	0.675	4.3.1.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	3hb3A	0.549	4.73	0.088	0.771	0.21	HEA	complex1.pdb.gz	107,112,115,119,137,140,141,182,190,191,194,195
2	0.01	1ar1A	0.551	4.81	0.083	0.779	0.15	HEA	complex2.pdb.gz	129,130,225,230,233,234,237,238
3	0.01	3ablN	0.553	4.73	0.037	0.779	0.10	UUU	complex3.pdb.gz	223,227,230,231
4	0.01	1m56G	0.543	4.95	0.051	0.786	0.17	PEH	complex4.pdb.gz	124,239,243,246,247
5	0.01	3omaA	0.550	4.74	0.078	0.771	0.16	HEA	complex5.pdb.gz	235,238,239,241,242
6	0.01	3om3A	0.546	4.76	0.073	0.775	0.15	HEA	complex6.pdb.gz	52,59,93,96,99,100,103,107,110,151,154
7	0.01	1v54A	0.554	4.73	0.037	0.779	0.34	HEA	complex7.pdb.gz	69,70,96,101,104,105,108,144,148,175,179,180
8	0.01	2occA	0.553	4.73	0.037	0.779	0.11	HEA	complex8.pdb.gz	100,104,107,108,111,116
9	0.01	2y69N	0.554	4.74	0.037	0.779	0.22	HEA	complex9.pdb.gz	94,97,98,101,104,108,109,148,155,156
10	0.01	1m57A	0.544	4.94	0.051	0.786	0.17	PEH	complex10.pdb.gz	227,228,229,232,236

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.