D-I-TASSER Q7Z434

D-I-TASSER results for Q7Z434

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q7Z434
Protein Name: Mitochondrial antiviral-signaling protein
Gene Name: MAVS

Input Sequence in FASTA format

>Q7Z434 (540 residues)
MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFN
TLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPP
TPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLES
SSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRL
PGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPT
TLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVP
SKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSK
PGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLE
GNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q7Z434-D1	1-540	540		MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.5452

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6z2wE	0.43	7.21	0.036	0.672	model1_6z2wE.pdb.gz
2	5x6oC	0.42	7.30	0.035	0.663	model1_5x6oC.pdb.gz
3	6bcuA	0.40	7.79	0.061	0.665	model1_6bcuA.pdb.gz
4	6emkA	0.39	7.76	0.061	0.652	model1_6emkA.pdb.gz
5	5fvmA	0.39	7.57	0.030	0.622	model1_5fvmA.pdb.gz
6	5h64A	0.39	7.79	0.038	0.646	model1_5h64A.pdb.gz
7	5h64B	0.37	7.24	0.059	0.589	model1_5h64B.pdb.gz
8	6ez8A	0.37	7.59	0.048	0.615	model1_6ez8A.pdb.gz
9	5wbiA	0.37	7.37	0.040	0.593	model1_5wbiA.pdb.gz
10	3hmjG	0.37	7.79	0.052	0.624	model1_3hmjG.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0019899	0.57	enzyme binding
GO:0004871	0.56	signal transducer activity
GO:0019901	0.55	protein kinase binding
GO:0050700	0.54	CARD domain binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0050794	1.00	regulation of cellular process
GO:0048518	1.00	positive regulation of biological process
GO:0080090	0.88	regulation of primary metabolic process
GO:0060255	0.88	regulation of macromolecule metabolic process
GO:0031323	0.88	regulation of cellular metabolic process
GO:0006355	0.87	regulation of transcription, DNA-templated
GO:0010604	0.77	positive regulation of macromolecule metabolic process
GO:0051090	0.76	regulation of sequence-specific DNA binding transcription factor activity
GO:0048522	0.76	positive regulation of cellular process
GO:0044093	0.76	positive regulation of molecular function
GO:0010628	0.76	positive regulation of gene expression
GO:0051091	0.75	positive regulation of sequence-specific DNA binding transcription factor activity
GO:0031325	0.74	positive regulation of cellular metabolic process
GO:0048583	0.73	regulation of response to stimulus
GO:0045893	0.73	positive regulation of transcription, DNA-templated
GO:0002376	0.73	immune system process
GO:0051239	0.72	regulation of multicellular organismal process
GO:0045944	0.72	positive regulation of transcription from RNA polymerase II promoter
GO:0002682	0.72	regulation of immune system process
GO:0031347	0.71	regulation of defense response
GO:0001817	0.71	regulation of cytokine production
GO:0050896	0.70	response to stimulus
GO:0009987	0.69	cellular process
GO:0001819	0.69	positive regulation of cytokine production
GO:0051704	0.68	multi-organism process
GO:0006952	0.68	defense response
GO:0098542	0.67	defense response to other organism
GO:0007165	0.61	signal transduction
GO:0048584	0.60	positive regulation of response to stimulus
GO:0050776	0.59	regulation of immune response
GO:0002684	0.59	positive regulation of immune system process
GO:0050792	0.58	regulation of viral process
GO:0002218	0.58	activation of innate immune response
GO:0032479	0.57	regulation of type I interferon production
GO:0045087	0.56	innate immune response
GO:0032648	0.56	regulation of interferon-beta production
GO:0042742	0.55	defense response to bacterium
GO:0032728	0.55	positive regulation of interferon-beta production
GO:0032727	0.55	positive regulation of interferon-alpha production
GO:0016032	0.55	viral process
GO:0002230	0.55	positive regulation of defense response to virus by host
GO:0051607	0.54	defense response to virus

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044444	1.00	cytoplasmic part
GO:0044446	0.87	intracellular organelle part
GO:0016020	0.87	membrane
GO:0031966	0.85	mitochondrial membrane
GO:0043226	0.73	organelle
GO:0043229	0.72	intracellular organelle
GO:0043231	0.71	intracellular membrane-bounded organelle
GO:0005739	0.65	mitochondrion
GO:0098805	0.52	whole membrane
GO:0098588	0.52	bounding membrane of organelle
GO:0005741	0.50	mitochondrial outer membrane

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2nztA	0.299	7.63	0.049	0.489	2.7.1.1	NA
2	0.060	1dgjA	0.313	7.70	0.042	0.518	1.2.-.-	459
3	0.060	2vuaA	0.196	6.88	0.018	0.298	3.4.24.69	NA
4	0.060	2pziA	0.304	7.90	0.049	0.509	2.7.11.1	NA
5	0.060	1qhaA	0.304	7.59	0.045	0.493	2.7.1.1	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

No Binding sites was predicted

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.