D-I-TASSER Q4V9L6

D-I-TASSER results for Q4V9L6

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q4V9L6
Protein Name: Transmembrane protein 119
Gene Name: TMEM119

Input Sequence in FASTA format

>Q4V9L6 (283 residues)
MVSAAAPSLLILLLLLLGSVPATDARSVPLKATFLEDVAGSGEAEGSSASSPSLPPPWTP
ALSPTSMGPQPITLGGPSPPTNFLDGIVDFFRQYVMLIAVVGSLAFLLMFIVCAAVITRQ
KQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALDSSRQLQADILAAT
QNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVL
EGAVVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPSV

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q4V9L6-D1	1-283	283		MVSAAAPSLLILLLLLLGSVPATDARSVPLKATFLEDVAGSGEAEGSSASSPSLPPPWTPALSPTSMGPQPITLGGPSPPTNFLDGIVDFFRQYVMLIAVVGSLAFLLMFIVCAAVITRQKQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALDSSRQLQADILAATQNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQEVQGHGVPVETPEAQEEPCSGVLEGAVVAGEGQGELEGSLLLAQEAQGPVGPPESPCACSSVHPSV

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.3959

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	4lglA2	0.44	6.45	0.061	0.760	model1_4lglA2.pdb.gz
2	6um1A	0.43	6.22	0.072	0.721	model1_6um1A.pdb.gz
3	2pi4A	0.43	6.42	0.069	0.735	model1_2pi4A.pdb.gz
4	1bxrA	0.42	6.63	0.068	0.760	model1_1bxrA.pdb.gz
5	6tjvP	0.42	4.73	0.034	0.590	model1_6tjvP.pdb.gz
6	5xh8A	0.42	6.15	0.061	0.714	model1_5xh8A.pdb.gz
7	5l9wb	0.42	5.79	0.039	0.685	model1_5l9wb.pdb.gz
8	5mrwA	0.42	5.88	0.088	0.693	model1_5mrwA.pdb.gz
9	1oa0A	0.42	6.27	0.068	0.689	model1_1oa0A.pdb.gz
10	1wyvB	0.31	6.34	0.081	0.541	model1_1wyvB.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0097159	0.63	organic cyclic compound binding
GO:1901363	0.62	heterocyclic compound binding
GO:0036094	0.54	small molecule binding
GO:0000166	0.53	nucleotide binding
GO:0043169	0.52	cation binding
GO:0035639	0.52	purine ribonucleoside triphosphate binding
GO:0032555	0.52	purine ribonucleotide binding
GO:0032550	0.52	purine ribonucleoside binding

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:1903012	1.00	positive regulation of bone development
GO:0048515	1.00	spermatid differentiation
GO:0045669	1.00	positive regulation of osteoblast differentiation
GO:0033690	1.00	positive regulation of osteoblast proliferation
GO:0030501	1.00	positive regulation of bone mineralization
GO:0007283	1.00	spermatogenesis

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0005886	1.00	plasma membrane
GO:0044424	0.71	intracellular part
GO:0044444	0.52	cytoplasmic part
GO:0043226	0.52	organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	1aroP	0.340	6.74	0.045	0.618	2.7.7.6	NA
2	0.060	1xc6A	0.353	6.47	0.057	0.618	3.2.1.23	NA
3	0.060	1mswD	0.321	6.58	0.046	0.576	2.7.7.6	NA
4	0.060	3hkzJ	0.381	6.37	0.062	0.657	2.7.7.6	NA
5	0.060	2vdcA	0.380	6.71	0.048	0.693	1.4.1.13	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
1	0.01	1ce8A	0.415	6.53	0.061	0.731	0.16	IMP	complex1.pdb.gz	136,142,143,145,146
2	0.01	1ce8E	0.406	6.63	0.057	0.728	0.10	ADP	complex2.pdb.gz	140,141,145
3	0.01	1jdbE	0.372	7.04	0.064	0.707	0.11	PO4	complex3.pdb.gz	140,142,143
4	0.01	1a9xA	0.413	6.68	0.061	0.742	0.19	ADP	complex4.pdb.gz	141,222,223,224
5	0.01	1t36A	0.413	6.80	0.056	0.760	0.18	U	complex5.pdb.gz	137,138,149,150
6	0.01	3e3jB	0.372	6.36	0.066	0.643	0.32	QNA	complex6.pdb.gz	147,148,149
7	0.01	1bxrA	0.424	6.63	0.068	0.760	0.11	ANP	complex7.pdb.gz	218,219,220,222
8	0.01	1ce8C	0.407	6.43	0.069	0.714	0.12	ADP	complex8.pdb.gz	138,144,146

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.