D-I-TASSER Q2M3C6

D-I-TASSER results for Q2M3C6

[Click result.zip to download all results on this page]

Summary of the Protein

Uniprot ID: Q2M3C6
Protein Name: Transmembrane protein 266
Gene Name: TMEM266

Input Sequence in FASTA format

>Q2M3C6 (531 residues)
MAVAPSFNMTNPQPAIEGGISEVEIISQQVDEETKSIAPVQLVNFAYRDLPLAAVDLSTA
GSQLLSNLDEDYQREGSNWLKPCCGKRAAVWQVFLLSASLNSFLVACVILVVILLTLELL
IDIKLLQFSSAFQFAGVIHWISLVILSVFFSETVLRIVVLGIWDYIENKIEVFDGAVIIL
SLAPMVASTVANGPRSPWDAISLIIMLRIWRVKRVIDAYVLPVKLEMEMVIQQYEKAKVI
QDEQLERLTQICQEQGFEIRQLRAHLAQQDLDLAAEREAALQAPHVLSQPRSRFKVLEAG
TWDEETAAESVVEELQPSQEATMKDDMNSYISQYYNGPSSDSGVPEPAVCMVTTAAIDIH
QPNISSDLFSLDMPLKLGGNGTSATSESASRSSVTRAQSDSSQTLGSSMDCSTAREEPSS
EPGPSPPPLPSQQQVEEATVQDLLSSLSEDPCPSQKALDPAPLARPSPAGSAQTSPELEH
RVSLFNQKNQEGFTVFQIRPVIHFQPTVPMLEDKFRSLESKEQKLHRVPEA

Predicted Contact and Distance Map Used in D-I-TASSER simulation

Contact Map

Distance Map

D-I-TASSER simulation is guided by the consensus contact map (left figure) and distance map (right figure) derived based on confidence scores of AttentionPotential. In the contact, distance map and hydrogen bond networks, the axes mark the residue index along the sequence. For the contact map, each dot represents a residue pair with predicted contact, while for the distance map and hydrogen bond network, a color scale represents a distance of 1-20+ angstroms or a angle of 0-180 degree.

Information of Domain

No. of Domain	Domain	Domain Boundary	Length of Domain	Models of Domain	Sequence
1	Q2M3C6-D1	1-531	531		MAVAPSFNMTNPQPAIEGGISEVEIISQQVDEETKSIAPVQLVNFAYRDLPLAAVDLSTAGSQLLSNLDEDYQREGSNWLKPCCGKRAAVWQVFLLSASLNSFLVACVILVVILLTLELLIDIKLLQFSSAFQFAGVIHWISLVILSVFFSETVLRIVVLGIWDYIENKIEVFDGAVIILSLAPMVASTVANGPRSPWDAISLIIMLRIWRVKRVIDAYVLPVKLEMEMVIQQYEKAKVIQDEQLERLTQICQEQGFEIRQLRAHLAQQDLDLAAEREAALQAPHVLSQPRSRFKVLEAGTWDEETAAESVVEELQPSQEATMKDDMNSYISQYYNGPSSDSGVPEPAVCMVTTAAIDIHQPNISSDLFSLDMPLKLGGNGTSATSESASRSSVTRAQSDSSQTLGSSMDCSTAREEPSSEPGPSPPPLPSQQQVEEATVQDLLSSLSEDPCPSQKALDPAPLARPSPAGSAQTSPELEHRVSLFNQKNQEGFTVFQIRPVIHFQPTVPMLEDKFRSLESKEQKLHRVPEA

Top 1 final models from D-I-TASSER

Click to view	Rank^a	Download	Estimated TM-score^b
	1	model1.pdb.gz	0.4174

(a)

D-I-TASSER simulations generate a large ensemble of structural conformations, i.e. decoys. These decoys are clustered by SPICKER based on pairwise structure similarity to report up to five final models from the five largest clusters. Models are ranked in descending order of cluster size. If the simulations converge well, it is possible to have less than 5 models generated, which is usually an indication of good model quality.

(b)

The model confidence is quantitatified by estimated TM-score (eTM-score), calculated based on significance of threading template alignments, contact map satisfaction rate, mean absolute error between distance of model and distance of AttentionPotential, and convergence of D-I-TASSER simulations. eTM-score is typically in the range of [0, 1], with higher eTM-score signifies higher model confidence.

Proteins with similar structure

Top 10 structural analogs in PDB (as identified by TM-align)

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	Download Alignment
1	6jxaA	0.43	7.61	0.055	0.706	model1_6jxaA.pdb.gz
2	4jspB	0.42	7.33	0.047	0.670	model1_4jspB.pdb.gz
3	5h64A	0.42	7.35	0.028	0.667	model1_5h64A.pdb.gz
4	6zwmA	0.42	6.72	0.038	0.633	model1_6zwmA.pdb.gz
5	2j5wA	0.42	7.69	0.048	0.693	model1_2j5wA.pdb.gz
6	4bxsV	0.41	7.61	0.035	0.682	model1_4bxsV.pdb.gz
7	7kveB	0.41	7.39	0.057	0.661	model1_7kveB.pdb.gz
8	1llwA	0.41	7.68	0.051	0.670	model1_1llwA.pdb.gz
9	6z2wE	0.40	7.50	0.054	0.659	model1_6z2wE.pdb.gz
10	6r9tA	0.40	7.75	0.060	0.682	model1_6r9tA.pdb.gz

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Gene Ontology (GO) Terms

Molecular Function (MF)

GO term	Cscore^GO	Name
GO:0005216	0.98	ion channel activity
GO:0005261	0.97	cation channel activity
GO:0005244	0.91	voltage-gated ion channel activity
GO:0046873	0.86	metal ion transmembrane transporter activity
GO:0022843	0.80	voltage-gated cation channel activity
GO:0015077	0.67	monovalent inorganic cation transmembrane transporter activity
GO:1901363	0.55	heterocyclic compound binding
GO:0097159	0.55	organic cyclic compound binding
GO:0004672	0.52	protein kinase activity
GO:0052813	0.51	phosphatidylinositol bisphosphate kinase activity
GO:0052742	0.51	phosphatidylinositol kinase activity
GO:0035639	0.51	purine ribonucleoside triphosphate binding
GO:0035004	0.51	phosphatidylinositol 3-kinase activity
GO:0032559	0.51	adenyl ribonucleotide binding
GO:0032550	0.51	purine ribonucleoside binding
GO:0016307	0.51	phosphatidylinositol phosphate kinase activity

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Molecular Function. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Biological Process (BP)

GO term	Cscore^GO	Name
GO:0065007	0.95	biological regulation
GO:0065008	0.88	regulation of biological quality
GO:0044699	0.79	single-organism process
GO:0042391	0.77	regulation of membrane potential
GO:0009987	0.75	cellular process
GO:0050789	0.71	regulation of biological process
GO:0086010	0.69	membrane depolarization during action potential
GO:0044763	0.66	single-organism cellular process
GO:0006810	0.63	transport
GO:0071704	0.62	organic substance metabolic process
GO:0050794	0.62	regulation of cellular process
GO:0044238	0.62	primary metabolic process
GO:0044767	0.60	single-organism developmental process
GO:0044260	0.58	cellular macromolecule metabolic process
GO:0006796	0.57	phosphate-containing compound metabolic process
GO:0006464	0.57	cellular protein modification process
GO:0050896	0.56	response to stimulus
GO:0006629	0.55	lipid metabolic process
GO:0007165	0.54	signal transduction
GO:0009653	0.53	anatomical structure morphogenesis
GO:0048646	0.52	anatomical structure formation involved in morphogenesis
GO:0044249	0.52	cellular biosynthetic process
GO:0035556	0.52	intracellular signal transduction
GO:0008610	0.52	lipid biosynthetic process
GO:0048514	0.51	blood vessel morphogenesis
GO:0048017	0.51	inositol lipid-mediated signaling
GO:0046834	0.51	lipid phosphorylation
GO:0006661	0.51	phosphatidylinositol biosynthetic process

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Biological Process. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Cellular Component (CC)

GO term	Cscore^GO	Name
GO:0044424	1.00	intracellular part
GO:0016020	1.00	membrane
GO:0005942	1.00	phosphatidylinositol 3-kinase complex
GO:0005886	0.94	plasma membrane
GO:0044444	0.65	cytoplasmic part
GO:0005737	0.59	cytoplasm
GO:0043226	0.55	organelle
GO:0043231	0.54	intracellular membrane-bounded organelle

Download full result of the above consensus prediction.

Click the graph to show a high resolution version.

(a)

Cscore^GO is the confidence score of predicted GO terms. Cscore^GO values range in between [0-1]; where a higher value indicates a better confidence in predicting the function using the template.

(b)

The graph shows the predicted terms within the Gene Ontology hierachy for Cellular Component. Confidently predicted terms are color coded by Cscore^GO:

[0.4,0.5)

[0.5,0.6)

[0.6,0.7)

[0.7,0.8)

[0.8,0.9)

[0.9,1.0]

Predicted Enzyme Commission (EC) Numbers

Top 5 enzyme homologs in PDB

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	EC Number	Predicted Active Site Residues
1	0.060	2rd0A	0.371	7.88	0.040	0.635	2.7.1.153	NA
2	0.060	1llwA	0.406	7.68	0.051	0.670	1.4.7.1	NA
3	0.060	3ebgA	0.370	7.81	0.026	0.625	3.4.11.-	NA
4	0.060	2d5lA	0.362	7.86	0.046	0.616	3.4.14.-	NA
5	0.060	2gtqA	0.379	7.94	0.043	0.650	3.4.11.2	NA

	Click on the radio buttons to visualize predicted active site residues.
(a)	Cscore^EC is the confidence score for the Enzyme Commission (EC) number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted Ligand Binding Sites

Template proteins with similar binding site:

Click to view	Rank	Cscore^LB	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov.	BS-score	Lig. Name	Download Complex	Predicted binding site residues
	1	0.01	2ckjA	0.381	7.81	0.041	0.638	0.48	FES	complex1.pdb.gz	107,155,175,176

	Click on the radio buttons to visualize predicted binding site and residues.
(a)	Cscore^LB is the confidence score of predicted binding site. Cscore^LB values range in between [0-1]; where a higher score indicates a more reliable ligand-binding site prediction.
(b)	BS-score is a measure of local similarity (sequence & structure) between template binding site and predicted binding site in the query structure. Based on large scale benchmarking analysis, we have observed that a BS-score >1 reflects a significant local match between the predicted and template binding site.
(c)	TM-score is a measure of global structural similarity between query and template protein.
(d)	RMSD^a the RMSD between residues that are structurally aligned by TM-align.
(e)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(f)	Cov. represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

[Click result.zip to download all results on this page]

References:
1.	Wei Zheng, Chengxin Zhang, Yang Li, Robin Pearce, Eric W. Bell, Yang Zhang. Folding non-homology proteins by coupling deep-learning contact maps with I-TASSER assembly simulations. In preparation, 2020.